##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522975_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5781620
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.665419899612914	34.0	31.0	34.0	30.0	35.0
2	32.7214431249373	34.0	31.0	34.0	31.0	34.0
3	33.0003651225781	34.0	31.0	34.0	31.0	36.0
4	35.78856289413694	37.0	35.0	37.0	33.0	37.0
5	35.8427797053421	37.0	35.0	37.0	35.0	37.0
6	35.91592391059945	37.0	35.0	37.0	35.0	37.0
7	35.73879466308751	37.0	35.0	37.0	35.0	37.0
8	35.785370882209484	37.0	36.0	37.0	34.0	37.0
9	37.0088022042265	39.0	36.0	39.0	34.0	39.0
10-11	37.12256313974284	38.5	36.0	39.0	34.0	39.0
12-13	36.98825813526312	38.0	36.5	39.0	33.5	39.0
14-15	38.09102907143672	39.0	36.5	41.0	34.5	41.0
16-17	38.10833425579682	39.0	37.0	41.0	34.5	41.0
18-19	38.10126020042826	39.0	37.0	41.0	34.0	41.0
20-21	37.9813304921458	39.0	36.5	41.0	34.0	41.0
22-23	37.90047097526299	39.0	36.5	41.0	34.0	41.0
24-25	37.76916954071696	39.0	36.0	41.0	34.0	41.0
26-27	37.646836180862806	39.0	36.5	41.0	34.0	41.0
28-29	37.6059959838246	39.0	36.5	41.0	34.0	41.0
30-31	37.451858562133104	38.0	36.0	41.0	34.0	41.0
32-33	37.31157011356679	38.0	36.5	41.0	33.0	41.0
34-35	37.1427720085374	38.0	36.0	41.0	32.5	41.0
36-37	37.167914010260105	38.0	35.5	41.0	33.0	41.0
38-39	37.3111424652606	38.0	36.0	41.0	33.0	41.0
40-41	37.214474749291725	38.0	36.5	41.0	33.0	41.0
42-43	37.25296768033873	38.0	36.5	41.0	33.0	41.0
44-45	37.09936747831922	38.0	36.0	41.0	33.0	41.0
46-47	36.85125855729017	38.0	36.0	41.0	32.0	41.0
48-49	36.82296890836824	38.0	36.5	41.0	32.0	41.0
50-51	36.4369156914498	38.0	35.0	40.0	31.0	41.0
52-53	36.48845730089491	38.0	35.5	40.0	31.0	41.0
54-55	36.29096715107531	37.5	35.5	40.0	31.0	41.0
56-57	35.99361372764035	37.0	35.0	40.0	29.5	41.0
58-59	35.85806849983223	36.5	35.0	40.0	30.0	41.0
60-61	35.58636835350646	36.5	35.0	40.0	29.0	41.0
62-63	35.39344067925599	37.5	34.5	39.5	28.5	41.0
64-65	35.24841895524092	37.0	35.0	39.0	28.0	41.0
66-67	35.02068918400033	36.5	34.0	39.0	28.5	41.0
68-69	34.55168127618211	36.0	34.0	38.0	27.0	40.0
70-71	34.30865933769428	36.0	34.0	37.5	26.5	40.0
72-73	34.044178621216886	36.0	34.0	37.0	26.0	39.5
74-75	33.76430490070257	35.5	33.5	37.0	26.0	39.0
76-77	32.290543221450044	34.5	31.0	36.0	24.5	37.5
78-79	33.29401378852294	35.0	33.0	37.0	26.0	37.0
80-81	33.294274960997086	35.0	33.0	36.5	26.0	37.0
82-83	33.11831095782843	35.0	33.0	36.0	26.0	37.5
84-85	32.91995418239179	35.0	33.0	36.0	25.5	37.0
86-87	32.67610747506754	35.0	33.0	36.0	25.0	37.0
88-89	32.51768898682376	35.0	33.0	36.0	25.0	36.5
90-91	32.38183398424663	35.0	32.5	35.5	25.0	37.0
92-93	32.232119284906304	35.0	32.0	35.5	24.0	36.5
94-95	32.11352207512773	35.0	32.0	35.0	24.5	36.0
96-97	31.940362216818123	35.0	32.0	35.0	23.5	36.5
98-99	31.812503588959494	35.0	31.5	35.0	23.0	36.5
100	30.607358145294917	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.10424177944997126
1101	2	-0.07649985902654066
1101	3	-0.08354191128305644
1101	4	-0.06255614050301261
1101	5	-0.11941069018982375
1101	6	-0.18961108304400653
1101	7	-0.11573600216644309
1101	8	-0.09073545640339375
1101	9	-0.09883156718431252
1101	10-11	-0.05435703665687441
1101	12-13	-0.08677435438470837
1101	14-15	-0.2407410552316307
1101	16-17	-0.09065712697741901
1101	18-19	-0.11169250494857863
1101	20-21	-0.10161412110620915
1101	22-23	-0.09535235604145953
1101	24-25	-0.218090680096644
1101	26-27	-0.25077392905149765
1101	28-29	-0.2972179118468077
1101	30-31	-0.251178245320574
1101	32-33	-0.13766281536225478
1101	34-35	-0.10589755832540959
1101	36-37	-0.2657058584670722
1101	38-39	-0.18859816745907665
1101	40-41	-0.24312523536965358
1101	42-43	-0.279235234835987
1101	44-45	-0.22600713237190462
1101	46-47	-0.25832982640702085
1101	48-49	-0.39354280869533653
1101	50-51	-0.2880456135290501
1101	52-53	-0.39987913085982996
1101	54-55	-0.3816025826272593
1101	56-57	-0.2773008428566044
1101	58-59	-0.451330923130449
1101	60-61	-0.4051848591055105
1101	62-63	-0.3893017781741719
1101	64-65	-0.3550389208839775
1101	66-67	-0.44046144412767063
1101	68-69	-0.3573458817874595
1101	70-71	-0.45465036083763977
1101	72-73	-0.5324225866777539
1101	74-75	-0.5674700354966902
1101	76-77	-0.48387347689662263
1101	78-79	-0.4451017573907734
1101	80-81	-0.3615002953437809
1101	82-83	-0.385191138125208
1101	84-85	-0.4920905188234528
1101	86-87	-0.5065725395246545
1101	88-89	-0.4445162651390646
1101	90-91	-0.5041061436158003
1101	92-93	-0.7429696313505758
1101	94-95	-0.6604863788801545
1101	96-97	-0.6198421349403027
1101	98-99	-0.5900432853848088
1101	100	-0.6489682162988935
1102	1	-0.036698141706487775
1102	2	-0.10755178050744973
1102	3	-0.1290385265880758
1102	4	-0.06817431571590049
1102	5	-0.11399647795800405
1102	6	-0.11829324252821749
1102	7	-0.1326744081358271
1102	8	-0.0523623924508243
1102	9	-0.10678311186945422
1102	10-11	-0.07539640144882043
1102	12-13	-0.08334616590187238
1102	14-15	-0.15154486401997502
1102	16-17	-0.1412677320380098
1102	18-19	-0.19006846635475227
1102	20-21	-0.13993148674393296
1102	22-23	-0.08708690998260238
1102	24-25	-0.21641239191476558
1102	26-27	-0.2010188494729661
1102	28-29	-0.16023643399330467
1102	30-31	-0.14815627095638462
1102	32-33	-0.15170102561087617
1102	34-35	-0.20828865496404347
1102	36-37	-0.3564467506672173
1102	38-39	-0.2508479292275254
1102	40-41	-0.28892796950069766
1102	42-43	-0.2956463771618232
1102	44-45	-0.3487664719046535
1102	46-47	-0.2659717334767464
1102	48-49	-0.21823120562999776
1102	50-51	-0.155021489850256
1102	52-53	-0.23170406062669713
1102	54-55	-0.28925826369685836
1102	56-57	-0.21100030464032926
1102	58-59	-0.39734571979831657
1102	60-61	-0.33393996214263666
1102	62-63	-0.2857220598421648
1102	64-65	-0.3322112027142836
1102	66-67	-0.40516228045831326
1102	68-69	-0.3675578206911325
1102	70-71	-0.30654131387162664
1102	72-73	-0.4153396884379674
1102	74-75	-0.7806676830433332
1102	76-77	-0.3619058491249376
1102	78-79	-0.35266352482479135
1102	80-81	-0.20830147803741994
1102	82-83	-0.13690429719287778
1102	84-85	-0.1968071548354473
1102	86-87	-0.27153769716959175
1102	88-89	-0.2650932102846184
1102	90-91	-0.2018874518065914
1102	92-93	-0.327841594968195
1102	94-95	-0.40841691861172436
1102	96-97	-0.4700129071782584
1102	98-99	-0.6369461484113366
1102	100	-0.5279680495526549
1103	1	-0.1178530506214841
1103	2	-0.1170504821726297
1103	3	-0.15504290666059006
1103	4	-0.08369846526935021
1103	5	-0.12394519307539298
1103	6	-0.05768186397970965
1103	7	-0.04278498787759588
1103	8	-0.06522918408714418
1103	9	-0.058838713726295566
1103	10-11	-0.07274923127538102
1103	12-13	-0.09290774675925206
1103	14-15	-0.14404482239264382
1103	16-17	-0.17574667167135516
1103	18-19	-0.18061051566430564
1103	20-21	-0.2179746424643625
1103	22-23	-0.15424911726571366
1103	24-25	-0.13966921419757483
1103	26-27	-0.2696406427437026
1103	28-29	-0.23100806027269272
1103	30-31	-0.22117807404240608
1103	32-33	-0.16048187475602305
1103	34-35	-0.1163555514136192
1103	36-37	-0.197847462450909
1103	38-39	-0.24433105963852597
1103	40-41	-0.29248918841931726
1103	42-43	-0.2868981726147197
1103	44-45	-0.3696151963991383
1103	46-47	-0.40062116993020425
1103	48-49	-0.32243539167527757
1103	50-51	-0.29164899176292636
1103	52-53	-0.2634152203468574
1103	54-55	-0.22959796031681634
1103	56-57	-0.06384579622416453
1103	58-59	-0.1717307963531809
1103	60-61	-0.30480213884061413
1103	62-63	-0.23757850774182288
1103	64-65	-0.3316558063650419
1103	66-67	-0.34714603790850873
1103	68-69	-0.2504235256348011
1103	70-71	-0.4769090892277177
1103	72-73	-0.45195400105666295
1103	74-75	-0.34652585913486433
1103	76-77	-0.32694556598892177
1103	78-79	-0.27499161924786364
1103	80-81	-0.26235797466297583
1103	82-83	-0.31254636604537467
1103	84-85	-0.2588148019968557
1103	86-87	-0.30755148063907
1103	88-89	-0.26508061253705506
1103	90-91	-0.27263935987239307
1103	92-93	-0.3458292066718762
1103	94-95	-0.02483544251501968
1103	96-97	-0.7878411518979398
1103	98-99	-1.051124157432639
1103	100	-0.2950672461888182
1104	1	-0.02720017698464261
1104	2	-0.07787908858565373
1104	3	-0.09214767111770783
1104	4	-0.1111440839815998
1104	5	-0.14421985725498132
1104	6	-0.11455298724396101
1104	7	-0.1330388718301947
1104	8	-0.08577571099023373
1104	9	-0.0656733524117783
1104	10-11	-0.145160935250928
1104	12-13	-0.12375391963952609
1104	14-15	-0.23667888565683626
1104	16-17	-0.07951193051123084
1104	18-19	-0.07104758330916638
1104	20-21	-0.20767903055941161
1104	22-23	-0.12784780174710164
1104	24-25	-0.14185970008210091
1104	26-27	-0.0639017777187334
1104	28-29	-0.12072925649075472
1104	30-31	-0.220256955769365
1104	32-33	-0.1581030912641026
1104	34-35	-0.04955998156732733
1104	36-37	-0.16961371180123308
1104	38-39	-0.2256804393148002
1104	40-41	-0.2818222271273285
1104	42-43	-0.24861858508771917
1104	44-45	-0.24132803340599196
1104	46-47	-0.19284124841883
1104	48-49	-0.3007440779009727
1104	50-51	-0.20478127429866788
1104	52-53	-0.1779141023719859
1104	54-55	-0.2912705191831293
1104	56-57	-0.23558229467523262
1104	58-59	-0.236619829916485
1104	60-61	-0.24827558363214308
1104	62-63	-0.09242225048470942
1104	64-65	-0.2709673770597547
1104	66-67	-0.2904158157375818
1104	68-69	-0.2405995013756339
1104	70-71	-0.2022658929466914
1104	72-73	-0.24425508310220323
1104	74-75	-0.2796901479690419
1104	76-77	-0.23972334100574244
1104	78-79	-0.2341488619843446
1104	80-81	-0.05896744585469804
1104	82-83	-0.021750943941221124
1104	84-85	-0.044328764580420454
1104	86-87	-0.1636051996333876
1104	88-89	-0.08117040380648888
1104	90-91	-0.0871442362527759
1104	92-93	-0.2319057643437361
1104	94-95	-0.283469369449044
1104	96-97	0.0010650512903325193
1104	98-99	-0.9483852954140417
1104	100	-0.20489112933214315
1105	1	-0.12158850470630256
1105	2	-1.0776839240226757
1105	3	-0.20920383759916206
1105	4	-0.14667730342286944
1105	5	-0.2119524652174718
1105	6	-0.15874310959500093
1105	7	-0.17966827478143443
1105	8	-0.15446036818063646
1105	9	-0.1779576623972403
1105	10-11	-0.13830474333726528
1105	12-13	-0.037394706268820244
1105	14-15	-0.14243762245005342
1105	16-17	-0.6783909782408557
1105	18-19	-0.1118458229579744
1105	20-21	-0.22832417936547955
1105	22-23	-0.16285357928160948
1105	24-25	-0.2560060900905583
1105	26-27	-0.23011674623255374
1105	28-29	-0.2609701990305524
1105	30-31	-0.20483978460507046
1105	32-33	-0.1755506926197441
1105	34-35	-0.1309662721690188
1105	36-37	-0.23457199744918
1105	38-39	-0.2282861350239358
1105	40-41	-0.20325909166763978
1105	42-43	-0.3390582858722482
1105	44-45	-0.37613228832519496
1105	46-47	-0.3308477502184388
1105	48-49	-0.3240236718702221
1105	50-51	-0.2560244117487045
1105	52-53	-0.37460266285365407
1105	54-55	-0.4263545284050636
1105	56-57	-0.27813618607969914
1105	58-59	-0.3717751598993928
1105	60-61	-0.3294176004063445
1105	62-63	-0.15881690308626162
1105	64-65	-0.22849696964681954
1105	66-67	-0.21888993846457794
1105	68-69	-0.21433715155006894
1105	70-71	-0.30569896477057057
1105	72-73	-0.31869460696701424
1105	74-75	-0.27520689176164836
1105	76-77	-0.2791062229173562
1105	78-79	-0.22868618928028894
1105	80-81	-0.1997167561442339
1105	82-83	-0.19031120445550442
1105	84-85	-0.13128950498943226
1105	86-87	-0.21671788090699096
1105	88-89	-0.19713077378984423
1105	90-91	-0.20081543095844978
1105	92-93	-0.2578792374436496
1105	94-95	-0.31785122854354597
1105	96-97	-0.18792335493945345
1105	98-99	-0.20010390460392102
1105	100	-0.2220202751969218
1106	1	-0.05181145238289986
1106	2	-0.08166207896270805
1106	3	-0.0682295449634509
1106	4	-0.047629647362271044
1106	5	-0.0799225439255764
1106	6	-0.016066219943645876
1106	7	-0.07907995348657693
1106	8	-0.034210012393380396
1106	9	-0.07888613296544378
1106	10-11	-0.08556115629846772
1106	12-13	-0.07273627128859772
1106	14-15	-0.08182590895271602
1106	16-17	0.023429412579183406
1106	18-19	-0.060894034213255566
1106	20-21	-0.028948588314477774
1106	22-23	-0.040928293680210004
1106	24-25	0.006693988191891265
1106	26-27	-0.008313510928090295
1106	28-29	-0.05241668474076988
1106	30-31	-0.04237335075003301
1106	32-33	-0.003732559591398399
1106	34-35	0.015435140986497231
1106	36-37	0.03568455801199377
1106	38-39	-0.013879943492881353
1106	40-41	-0.04348405655158416
1106	42-43	-0.041890889124573505
1106	44-45	-0.1283712959332064
1106	46-47	-0.1194471050822159
1106	48-49	-0.13119151277363272
1106	50-51	-0.06181453705614359
1106	52-53	-0.14233780168008536
1106	54-55	-0.08519183970827982
1106	56-57	-0.05001511292539362
1106	58-59	-0.13392254187888852
1106	60-61	-0.04952166239568356
1106	62-63	-0.09842421388979261
1106	64-65	-0.08883450950516902
1106	66-67	-0.11342784067998934
1106	68-69	-0.033189712200453414
1106	70-71	-0.023384321140781594
1106	72-73	-0.10076505747048259
1106	74-75	-0.06895678766264979
1106	76-77	-0.09933631409882793
1106	78-79	-0.12420521302596654
1106	80-81	0.04639873175158726
1106	82-83	-0.025670535705003772
1106	84-85	0.10193056862937766
1106	86-87	0.04286857308310488
1106	88-89	0.03696558209734491
1106	90-91	-0.03817697928537456
1106	92-93	-0.052837884693310855
1106	94-95	-0.0019283376818677311
1106	96-97	-0.15126468307616392
1106	98-99	-0.17274955530133695
1106	100	-0.11454032235145561
1107	1	0.020862416159921793
1107	2	-0.03228518316628737
1107	3	0.007172980280387264
1107	4	-0.06808292547739825
1107	5	-0.07080749022686916
1107	6	-0.043847700915442545
1107	7	-0.0700353202031252
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2316	90-91	-0.036695023414246464
2316	92-93	-0.15147923393161022
2316	94-95	-0.04405522892987079
2316	96-97	0.060362527584427284
2316	98-99	0.1697306810274668
2316	100	-0.18522253853836546
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	7.0
12	20.0
13	105.0
14	385.0
15	1204.0
16	2531.0
17	5005.0
18	8318.0
19	12546.0
20	17707.0
21	24060.0
22	31531.0
23	36925.0
24	40971.0
25	45160.0
26	51941.0
27	61879.0
28	75739.0
29	93440.0
30	116714.0
31	146274.0
32	187176.0
33	251863.0
34	382633.0
35	717350.0
36	1253612.0
37	961797.0
38	1035477.0
39	219128.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61282237119729	18.412108123670258	12.834430461042782	24.14063904408967
2	16.42224140097687	20.331660904385597	39.46747106656378	23.778626628073752
3	19.04152816392498	25.585741629210823	29.801258295029953	25.571471911834248
4	13.246670180100073	16.324337525527017	36.81550098683431	33.613491307538595
5	13.60648549731342	37.20709883136168	34.30433026637482	14.882085404950088
6	31.73697230127616	35.98172190717878	18.07269864254771	14.208607148997348
7	28.05111716093413	32.07068261144801	21.96086909897226	17.917331128645607
8	26.433375836014832	35.13827323804708	20.168583207820124	18.259767718117974
9	27.28920740021091	15.471790859965004	19.811215509012268	37.42778623081182
10-11	25.410133857846205	25.555586208256393	28.477570770191775	20.55670916370563
12-13	25.610143047388746	23.350532390125217	28.303404126407226	22.735920436078807
14-15	23.12139553239974	24.897129503392488	25.770656244359152	26.21081871984862
16-17	22.96247981840914	27.593947905313236	26.463908539698185	22.979663736579443
18-19	22.767917293582908	26.804692882100078	28.10376077101055	22.32362905330646
20-21	23.504061070952122	26.256437877176968	27.898508424047797	22.340992627823113
22-23	23.332458705396135	25.95846999034508	27.849387339561698	22.859683964697087
24-25	23.025152497425875	26.012202907409453	27.924900368809663	23.03774422635501
26-27	22.8677906850533	26.172697068987066	27.953289913802386	23.00622233215725
28-29	22.91839877394265	26.463292443132897	27.919676637752204	22.698632145172247
30-31	23.498783129650942	26.12614887843725	27.90417467095115	22.47089332096066
32-33	23.233385477709163	26.20016607668017	27.842787431276715	22.723661014333953
34-35	23.35945264047506	26.383094760389667	27.679174892462505	22.57827770667277
36-37	23.31589117922384	26.338154314286722	27.664347849550524	22.681606656938914
38-39	23.168265027168815	26.47173846676739	27.61252593426038	22.747470571803415
40-41	23.23084540326434	26.165984523678254	27.83063827395122	22.77253179910619
42-43	23.002254771151797	26.378902565035688	27.56751773496165	23.051324928850867
44-45	22.75048224214229	26.50832127151409	27.7177233327706	23.023473153573022
46-47	22.89931041497926	26.262391061931865	27.40804639259257	23.430252130496292
48-49	22.912086453039606	26.41817772449927	27.368333553757353	23.30140226870377
50-51	22.898059246671075	26.577208049753253	27.396274733395515	23.128457970180154
52-53	22.93396921252436	26.6513318556655	27.16624385808992	23.248455073720216
54-55	22.75503383477601	26.41187231489679	27.508887536502964	23.324206313824238
56-57	22.928525141501726	26.600655733281513	27.078458380758587	23.392360744458173
58-59	22.770718126448276	26.556379397752846	27.3477643226687	23.325138153130176
60-61	23.077041493194592	26.514798241957436	27.37103969087233	23.037120573975642
62-63	22.933531554274442	26.55317393498405	27.288952425676875	23.22434208506463
64-65	22.81984223558114	26.30015541204949	27.254101727867187	23.625900624502176
66-67	22.668525367039706	26.44311755907981	27.1087367392902	23.77962033459028
68-69	22.465875525929967	26.482152111296152	27.282523937986447	23.76944842478743
70-71	22.584388968365282	26.287158673105726	27.299578956726616	23.828873401802376
72-73	22.492900437303312	26.62782274426941	26.87819713831149	24.001079680115794
74-75	22.496609688679474	26.612871420713418	26.920343251723693	23.97017563888341
76-77	22.4520105074353	26.410815535319028	27.026116112297373	24.111057844948302
78-79	22.360714927245205	26.36355404595605	27.087828554176113	24.18790247262263
80-81	22.361154459871845	26.611252897232262	26.801866370623816	24.225726272272073
82-83	22.48886176043036	26.53356675415479	26.852529855117485	24.12504163029736
84-85	22.484780730350252	26.57070481825131	26.694918515624266	24.249595935774177
86-87	22.209371008318694	26.581992214897205	26.872510942125434	24.33612583465867
88-89	22.03584810399821	26.396201648655094	26.97077830710426	24.597171940242436
90-91	22.398354008545443	26.270483006233093	26.89655959696698	24.434603388254487
92-93	22.23844419430206	26.306905567251672	26.851288121826567	24.6033621166197
94-95	22.116702798795757	26.097904751625773	26.874572563312523	24.91081988626595
96-97	22.006518224348167	26.035190800975354	27.006585248679727	24.95170572599675
98-99	21.95585799504344	26.04185260675902	26.888130829870523	25.114158568327017
100	21.95464180727049	26.14097311770765	26.947450536772344	24.956934538249516
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1095.0
1	782.0
2	1061.5
3	1503.5
4	1061.5
5	878.5
6	1265.5
7	2274.5
8	3532.5
9	4316.0
10	4084.5
11	3643.5
12	3803.0
13	4083.5
14	4433.5
15	4844.5
16	5260.5
17	5638.0
18	6050.5
19	6514.0
20	7186.5
21	8273.5
22	9689.5
23	11605.5
24	14301.5
25	17782.5
26	22429.0
27	28404.5
28	35217.0
29	43002.5
30	51659.5
31	60497.0
32	70610.5
33	81527.5
34	92444.5
35	103598.0
36	118667.0
37	133264.0
38	143830.0
39	153528.5
40	160581.0
41	166533.5
42	174248.0
43	184208.0
44	193049.5
45	202570.5
46	212219.5
47	218793.0
48	228342.5
49	237839.0
50	242236.5
51	241484.5
52	235743.0
53	229506.0
54	221247.0
55	208059.0
56	190147.5
57	171310.5
58	154075.5
59	133772.5
60	111161.0
61	90960.0
62	73986.5
63	57573.5
64	42418.5
65	31662.5
66	23641.0
67	18021.5
68	13667.5
69	9952.0
70	7644.5
71	5942.5
72	4528.5
73	3439.0
74	2615.0
75	1903.5
76	1292.5
77	775.5
78	474.5
79	304.5
80	190.0
81	121.0
82	87.0
83	64.5
84	41.5
85	26.0
86	18.0
87	11.0
88	8.0
89	9.5
90	7.5
91	4.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03166932451458242
2	0.07599946035886136
3	0.002525243789802858
4	1.7296190341115467E-5
5	0.001816099985817124
6	9.339942784202353E-4
7	0.0
8	4.1510856818677115E-4
9	1.7296190341115467E-5
10-11	2.5944285511673197E-5
12-13	7.78328565350196E-5
14-15	0.011873834669175767
16-17	8.907538025674464E-4
18-19	2.2485047443450106E-4
20-21	2.9403523579896293E-4
22-23	0.0019717656988871633
24-25	8.12920946032427E-4
26-27	0.007394121370826861
28-29	0.008431892791293789
30-31	0.010403658490180953
32-33	0.009608033734489641
34-35	0.00457484234522504
36-37	0.0038743466364098643
38-39	0.0030268333096952066
40-41	0.003588959495781459
42-43	0.0017642114147937774
44-45	0.005050487579605716
46-47	0.018022630335442314
48-49	0.01893068032835088
50-51	0.01335265894334114
52-53	0.007575731369408574
54-55	0.0011847890383664093
56-57	0.002654965217361224
58-59	0.0029922409290129757
60-61	0.0032862761648119383
62-63	0.003329516640664727
64-65	0.0037186809233398254
66-67	0.00278468664491959
68-69	0.007471954227361882
70-71	5.015895198923484E-4
72-73	0.004808340914830099
74-75	0.007610323750090804
76-77	0.006581200424794435
78-79	0.004298103299767193
80-81	4.2375666335732893E-4
82-83	0.0012712699900719866
84-85	8.907538025674464E-4
86-87	0.0011242523721725051
88-89	0.0010204752301258124
90-91	0.0010204752301258124
92-93	4.1510856818677115E-4
94-95	0.0010723638011491587
96-97	0.0023782261719033763
98-99	0.003251683784129708
100	0.005275338054040217
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5781620.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.13620305090682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.48103493124809	45.99279046169033
2	14.136846440703831	17.0027253611531
3	4.3287557881027166	7.809448110933993
4	1.7955949825392596	4.319210578686816
5	0.9188406446217209	2.7627793788198964
6	0.555949122513666	2.0059601590473175
7	0.3587455412753782	1.5101516299620534
8	0.23865293322409223	1.1481345000846814
9	0.1781957303697722	0.9644413161889138
>10	0.9246222475155524	9.793231930169583
>50	0.05463028664559166	2.23250625939512
>100	0.026171554303490798	2.743389943976343
>500	0.001405137897723903	0.5357722003270132
>1k	4.437272312684667E-4	0.45854352132544396
>5k	3.697726927225136E-5	0.16103836775289407
>10k+	7.395453854450272E-5	0.5598762804863804
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13474	0.2330488686561898	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11704	0.20243461175241542	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7242	0.1252590104503582	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.4592380682230934E-5
2	0.0	0.0	0.0	0.0	3.4592380682230934E-5
3	0.0	0.0	0.0	0.0	3.4592380682230934E-5
4	0.0	0.0	0.0	0.0	3.4592380682230934E-5
5	1.7296190341115467E-5	0.0	0.0	0.0	3.4592380682230934E-5
6	3.4592380682230934E-5	0.0	0.0	0.0	6.918476136446187E-5
7	3.4592380682230934E-5	0.0	0.0	0.0	6.918476136446187E-5
8	3.4592380682230934E-5	0.0	0.0	0.0	6.918476136446187E-5
9	3.4592380682230934E-5	0.0	0.0	0.0	1.037771420466928E-4
10-11	3.4592380682230934E-5	0.0	0.0	1.7296190341115467E-5	1.037771420466928E-4
12-13	3.4592380682230934E-5	0.0	0.0	1.7296190341115467E-5	1.037771420466928E-4
14-15	3.4592380682230934E-5	0.0	0.0	1.7296190341115467E-5	1.1242523721725053E-4
16-17	3.4592380682230934E-5	0.0	0.0	1.7296190341115467E-5	1.2107333238780826E-4
18-19	3.4592380682230934E-5	0.0	0.0	1.7296190341115467E-5	1.556657130700392E-4
20-21	3.4592380682230934E-5	0.0	0.0	1.7296190341115467E-5	1.556657130700392E-4
22-23	3.4592380682230934E-5	0.0	0.0	1.556657130700392E-4	1.8160999858171238E-4
24-25	3.4592380682230934E-5	0.0	0.0	8.302171363735423E-4	2.2485047443450106E-4
26-27	3.4592380682230934E-5	0.0	0.0	0.0018333961761582394	2.4214666477561652E-4
28-29	3.4592380682230934E-5	0.0	0.0	0.005309930434722448	2.4214666477561652E-4
30-31	3.4592380682230934E-5	0.0	0.0	0.018420442713287973	2.507947599461743E-4
32-33	3.4592380682230934E-5	0.0	0.0	0.054405166717978695	2.7673904545784747E-4
34-35	3.4592380682230934E-5	0.0	0.0	0.11054860056523949	2.9403523579896293E-4
36-37	3.4592380682230934E-5	0.0	0.0	0.19473780705061905	3.2862761648119385E-4
38-39	3.4592380682230934E-5	0.0	0.0	0.31242108613156866	3.5457190199286704E-4
40-41	3.4592380682230934E-5	0.0	0.0	0.4471670569840287	3.6321999716342477E-4
42-43	3.4592380682230934E-5	0.0	0.0	0.577995440724226	3.8051618750454023E-4
44-45	3.4592380682230934E-5	0.0	0.0	0.7408477208809987	3.9781237784565574E-4
46-47	3.4592380682230934E-5	0.0	0.0	0.9317717179614018	3.9781237784565574E-4
48-49	3.4592380682230934E-5	0.0	0.0	1.136653740647085	4.151085681867712E-4
50-51	3.4592380682230934E-5	0.0	0.0	1.3472780985260187	4.151085681867712E-4
52-53	3.4592380682230934E-5	0.0	0.0	1.5620621901819907	4.410528536984444E-4
54-55	3.4592380682230934E-5	0.0	0.0	1.8020035906891148	4.669971392101176E-4
56-57	3.4592380682230934E-5	0.0	0.0	2.0805414399424382	4.756452343806753E-4
58-59	3.4592380682230934E-5	0.0	0.0	2.3878860942088895	5.015895198923486E-4
60-61	3.4592380682230934E-5	0.0	0.0	2.7094914574115903	5.015895198923486E-4
62-63	3.4592380682230934E-5	0.0	0.0	3.0335355834523887	5.18885710233464E-4
64-65	3.4592380682230934E-5	0.0	0.0	3.3616010737474964	5.18885710233464E-4
66-67	5.18885710233464E-5	0.0	0.0	3.735856040348553	5.275338054040217E-4
68-69	5.18885710233464E-5	0.0	0.0	4.159171650852183	5.361819005745795E-4
70-71	5.18885710233464E-5	0.0	0.0	4.607385127351849	5.534780909156949E-4
72-73	5.18885710233464E-5	0.0	0.0	5.039262352074331	6.572552329623877E-4
74-75	5.18885710233464E-5	0.0	0.0	5.475524161048288	6.572552329623877E-4
76-77	5.18885710233464E-5	0.0	0.0	5.953737879694619	6.572552329623877E-4
78-79	5.18885710233464E-5	0.0	0.0	6.486149210774835	6.572552329623877E-4
80-81	5.18885710233464E-5	0.0	0.0	7.028082094637835	6.659033281329454E-4
82-83	5.18885710233464E-5	0.0	0.0	7.584292983627426	6.918476136446186E-4
84-85	5.18885710233464E-5	0.0	0.0	8.147206146374199	6.918476136446186E-4
86-87	5.18885710233464E-5	0.0	0.0	8.714659559085515	7.177918991562918E-4
88	5.18885710233464E-5	0.0	0.0	9.15693179420301	7.43736184667965E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7735	0.0	51.999382	1
GTATCAA	13830	0.0	40.37578	1
TACCTGG	6235	0.0	39.447414	2
TCAACGC	17140	0.0	31.996426	4
GTACCTG	7845	0.0	31.7216	1
CAACGCA	17505	0.0	31.35611	5
ATCAACG	17570	0.0	31.24092	3
ACCTGGG	7965	0.0	30.327042	3
TATCAAC	18230	0.0	30.285498	2
AACGCAG	18380	0.0	29.939552	6
GTACATG	19660	0.0	28.809475	1
TACATGG	20300	0.0	27.428934	2
ACATGGG	20480	0.0	26.22823	3
ACGCAGA	21540	0.0	25.416626	7
CATGGGG	14825	0.0	24.471693	4
CGCAGAG	22565	0.0	24.26482	8
ATGGGGG	9260	0.0	23.3447	5
ATAACGC	565	0.0	22.457842	3
TGGGGGG	8405	0.0	22.364344	6
GCAGAGT	25075	0.0	21.70453	9
>>END_MODULE