FastQCFastQC Report
Mon 6 Jun 2016
ERR522974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7318652
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT390570.5336638495722983No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230330.3147164259210576No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT199090.27203096963757806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179110.244730860273176No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT112910.1542770444611931No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102340.1398344941117572No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC82480.11269834936816234No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC78570.10735583547352709No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG244850.026.5964281
TACATGG250400.025.384832
ACATGGG254300.024.1652323
TACCTGG55750.023.7813322
CATGGGG177600.022.2089024
TATCACG7150.021.0414582
ATGGGGG103950.020.5787435
GAGTACT211950.019.92907312-13
GTACCTG68650.019.793881
AGAGTAC319450.019.05270610-11
TATAACG6500.018.8058032
GTACTTT226750.018.36752514-15
AGTACTT224550.017.54489712-13
ATAACGC6850.017.1597583
GAGTACA188750.016.7649291
AGTACAT187800.015.8968122
ACCTGGG81450.015.7014423
TAACGCA7350.015.3508354
CATGGGA93650.015.2104814
ACTTTTT280750.015.10128316-17
Produced by FastQC (version 0.11.5)