FastQCFastQC Report
Mon 6 Jun 2016
ERR522974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7318652
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT403100.5507844887282521No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213150.29124215770882395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172260.23537121316876386No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT115550.15788426611895195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99440.13587201577558272No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC88010.12025438564369503No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC84100.1149118717490598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG245750.027.1936721
TACATGG251650.026.3152982
ACATGGG248900.025.7347933
CATGGGG179100.023.2218824
TACCTGG57850.022.8295422
ATGGGGG109450.021.940775
GTACCTG67500.019.9961051
GAGTACT211250.019.82909212-13
AGAGTAC312950.019.36414310-11
GTACTTT225500.018.34331514-15
AGTACTT221700.017.67509512-13
GAGTACA189000.017.492861
ATAACGC6150.016.8111463
AGTACAT185800.016.4169772
GGGTTAG21700.016.254321
ACCTGGG79800.016.2538383
GTATAGC21300.016.117981
GTATAGG19550.016.1174141
TGGGGGG111200.015.7634336
CGTATAC5450.015.5212163
Produced by FastQC (version 0.11.5)