FastQCFastQC Report
Mon 6 Jun 2016
ERR522973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747628
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338600.3870763594428112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284840.3256196994202314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171150.19565303874376003No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93180.10652030470431528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG108750.043.7164042
GTACCTG127200.037.9404451
ACCTGGG132100.035.2411423
GGTATCA312050.027.4943081
GTACATG401250.025.7899071
GTATCAA429950.025.282741
CCTGGGG128250.024.4543514
TACATGG416350.024.3620682
TATAACG6800.024.2036972
CATGGGG241150.024.0221084
ACATGGG416700.023.935073
ATGGGGG139950.022.308635
TCAACGC497450.021.475664
CAACGCA504000.021.1960775
ATCAACG505650.021.1567523
TATCAAC515750.020.8520852
AACGCAG515950.020.7575156
GAGTACT273400.020.70216812-13
TAACGCA9050.020.2629974
TGGGGGG143450.019.426426
Produced by FastQC (version 0.11.5)