FastQCFastQC Report
Mon 6 Jun 2016
ERR522973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747628
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304620.34823154345383683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282620.3230818685934061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169690.1939840148666587No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT95390.10904670386074945No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG109500.046.0097922
GTACCTG131100.039.4086231
ACCTGGG135450.036.115963
GGTATCA327250.027.0212481
TATAACG6950.025.696172
CCTGGGG126550.024.9908084
GTATCAA442850.024.7871441
GTACATG402950.024.1382391
TACATGG417400.022.9917982
CATGGGG241500.022.7664934
ACATGGG417650.022.1769353
TCAACGC510700.021.1727944
CAACGCA516550.020.9784975
ATCAACG519950.020.8051663
TAACGCA9300.020.717074
GAGTACT283100.020.60675212-13
ATGGGGG140600.020.5549875
AACGCAG529750.020.5445926
TATCAAC532950.020.3878292
TGGGGGG130300.019.7996646
Produced by FastQC (version 0.11.5)