FastQCFastQC Report
Mon 6 Jun 2016
ERR522972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294603
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182410.3445206373357927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147490.2785666838476841No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT131290.2479694889305204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78250.14779200631284348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG68300.041.1074032
GTACCTG78750.036.1299321
ACCTGGG75200.036.022273
TATAACG5800.035.677232
TAACGCA7450.027.77534
ATAACGC7500.026.9633393
GTACATG203700.025.07271
CCTGGGG70100.024.4200024
TACATGG207850.024.21032
ACATGGG209300.023.3235453
CATGGGG129550.022.8336644
TATCACG5100.022.1313292
ATGGGGG81650.021.771575
GAGTACT171300.021.19545612-13
GTATCAA351200.021.1575281
TCAACGC381000.019.2434734
CAACGCA384450.019.1193565
ATCAACG386650.018.986783
AGTACTT179850.018.89424312-13
AACGCAG391750.018.863276
Produced by FastQC (version 0.11.5)