FastQCFastQC Report
Mon 6 Jun 2016
ERR522972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294603
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171140.32323481099527196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145350.27452483217344154No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT129390.24438092903282835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76070.14367460600917575No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG73600.043.8133932
TATAACG5200.038.8710022
GTACCTG85400.038.832151
ACCTGGG81250.038.3550073
ATAACGC7000.028.873693
TAACGCA6350.028.1281874
CCTGGGG72700.027.3487724
GTACATG199800.024.4142531
TACATGG203950.023.0712412
ACATGGG203350.022.2825433
TATCACG5400.021.7624032
CATGGGG127250.021.2763234
ATGGGGG78800.021.2353695
GAGTACT172450.020.57606512-13
CTGGGGG47050.019.9805015
GTATAAC16650.019.4937341
GTATCAA350550.019.3765931
TGGGGGG80650.018.8807476
AGTACTT179750.018.43311712-13
GGTATCA278100.018.3184051
Produced by FastQC (version 0.11.5)