FastQCFastQC Report
Mon 6 Jun 2016
ERR522971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12069561
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT553440.45854194696890793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337310.27947163944073855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280910.23274251648423666No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT153040.12679831519969947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150220.12446185905187437No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG427500.024.1443651
TACATGG432350.023.5371342
ACATGGG443000.022.4192053
GAGTACT312300.020.8283812-13
CATGGGG273300.020.4755484
GTACTTT338800.018.93625614-15
TACCTGG108650.018.8274422
CCGTATC30650.018.70449494
TATAACG7950.018.3369182
AGTACTT328750.018.10634612-13
AGAGTAC542350.017.90248110-11
ATGGGGG152100.016.9417365
CGCCGTA40050.016.66105594
GTATAGG31000.016.2307831
ACTTTTT412650.015.72465216-17
GAGTACA358150.015.6636471
AGTACAT336450.015.2208592
ACCTGGG124250.015.0253333
TACTTTT393900.014.99871814-15
ATAACGC13200.014.6062913
Produced by FastQC (version 0.11.5)