FastQCFastQC Report
Mon 6 Jun 2016
ERR522971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12069561
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT565060.46816947194682557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318280.2637047030956635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284220.2354849526010101No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT174910.1449182783035771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150860.12499211860315382No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT148290.12286279509254729No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC128320.10631704003153056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG430950.022.6705821
TACATGG432100.021.9513132
TACCTGG101400.020.9982872
ACATGGG437850.020.985963
GAGTACT322050.020.70025412-13
CATGGGG273700.020.1948184
GTACTTT334850.019.68399414-15
AGTACTT336600.017.71111912-13
AGAGTAC560700.017.17878310-11
ATGGGGG147650.016.553165
GTATAGG31150.016.1576711
ACTTTTT413150.016.0217116-17
ACCTGGG124600.015.9562113
GTACCTG144500.015.6903361
TACTTTT392600.015.17254214-15
GTGTAGC66850.014.3542931
GAGTACA364900.014.2572021
TATACTG59800.014.0689885
GTATCAA1027600.013.9888591
TATAACG8750.013.9665432
Produced by FastQC (version 0.11.5)