FastQCFastQC Report
Mon 6 Jun 2016
ERR522970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10247315
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261170.25486676265929176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214130.2089620549382936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128070.12497907988580424No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146450.048.6068151
GTATCAA259700.039.4940951
TCAACGC315600.031.8645614
ATCAACG320700.031.434323
CAACGCA324100.031.0284125
TATCAAC334900.030.2882122
AACGCAG336850.029.9030386
GTACATG339950.026.8493841
ACGCAGA381450.026.332557
TACATGG346900.025.5413842
CGCAGAG396400.025.3264648
ACATGGG343050.025.139373
GCAGAGT445500.022.0914979
CATGGGG223600.022.0462534
AGAGTAC394650.019.95026210-11
GAGTACT252650.019.702712-13
CAGAGTA435200.018.98192410-11
GTACTTT273700.018.03315414-15
GAGTACA255500.017.9908331
ATGGGGG121450.017.7985695
Produced by FastQC (version 0.11.5)