FastQCFastQC Report
Mon 6 Jun 2016
ERR522970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10247315
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241940.23610087130140922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208110.20308734531923728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121730.11879209334347582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151400.050.5570221
GTATCAA268450.039.536251
TCAACGC327700.031.7927234
ATCAACG333950.031.2273773
CAACGCA334650.031.0903225
AACGCAG347950.029.9693136
TATCAAC350800.029.844222
ACGCAGA402200.025.8332357
GTACATG341250.025.6011221
CGCAGAG413850.025.0432688
TACATGG351750.024.3885152
ACATGGG351550.023.4344353
GCAGAGT464050.022.0502079
CATGGGG223550.021.231724
GAGTACT266000.020.79803712-13
AGAGTAC413150.019.92630210-11
CAGAGTA456550.019.14912210-11
GTACTTT287950.018.98206714-15
ATGGGGG120200.018.2188075
AGTACTT293750.017.80921412-13
Produced by FastQC (version 0.11.5)