FastQCFastQC Report
Mon 6 Jun 2016
ERR522969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989664
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91070.3046161709141897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68250.22828652316782086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39750.13295808492191766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG43900.043.4918562
ACCTGGG46300.040.6283
GTACCTG49450.040.131491
TAACGCA2750.030.7812464
CCTGGGG45200.028.8195864
GTATCAA125000.027.9147031
GGTATCA88300.025.7232361
TCAACGC139200.024.6958834
CAACGCA140250.024.5105825
ATAACGC3650.024.479763
ATCAACG142550.024.0495363
AACGCAG143950.024.0346626
TATAACG3550.023.844632
GTACATG106600.023.7336311
TATCAAC145050.023.6026122
TATCACG2704.7293724E-1122.6425842
TACATGG110300.022.4688852
ACATGGG109800.021.7146113
ACGCAGA159400.021.5252537
AATACCG2659.0039975E-1021.2948475
Produced by FastQC (version 0.11.5)