FastQCFastQC Report
Mon 6 Jun 2016
ERR522969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989664
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83860.2804997484667173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67450.22561063718197094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39910.13349326211908763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG41650.045.4798662
GTACCTG48800.038.9452131
ACCTGGG47500.038.5903173
TATAACG3200.036.7213972
TAACGCA3900.030.1288644
ATAACGC4000.029.3756433
CCTGGGG42700.028.7289664
TACCGTA2203.6379788E-1227.7724087
GTATCAA120550.025.7944811
GGTATCA86550.024.8937851
GTACATG104550.024.747491
TACATGG106650.023.7110392
ACATGGG106900.022.6431523
TCAACGC135050.022.5869454
CAACGCA136700.022.3143145
CATGGGG65700.022.2485854
AACGCAG141650.021.832076
ATCAACG139850.021.7780953
TATCAAC142800.021.526762
ATGGGGG35750.021.0354255
Produced by FastQC (version 0.11.5)