FastQCFastQC Report
Mon 6 Jun 2016
ERR522968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6452823
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225810.34993986352949713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175000.2711991325347061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103480.16036392134109365No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT91510.14181390067571975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG84650.042.502342
ACCTGGG94050.037.653713
GTACCTG122950.029.873821
TATAACG4650.028.3193592
CCTGGGG91850.027.8030364
GTACATG241150.024.9041561
GTATCAA340500.024.4331191
TACATGG246600.023.5723952
GGTATCA254300.023.3944261
ACATGGG247550.022.3796843
CATGGGG159000.021.6809564
TCAACGC384100.021.170074
GAGTACT202250.021.05385612-13
CAACGCA387400.020.9766185
ATCAACG388550.020.9155083
TATCAAC395000.020.800522
CGTATCA19100.020.6660394
AACGCAG396200.020.5718696
TAACGCA7350.020.4754874
TATAGCG2551.3400495E-820.2863485
Produced by FastQC (version 0.11.5)