FastQCFastQC Report
Mon 6 Jun 2016
ERR522968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6452823
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT222960.3455231919425033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214040.33169978472987716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177270.2747169727110135No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT117470.18204435485058246TruSeq Adapter, Index 1 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99550.15427356367902853No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT95620.14818320601696341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG82600.043.477322
ACCTGGG91900.037.7462233
TATAACG6000.032.903912
GTACCTG122400.029.706211
ATAACGC7800.027.7201713
CCTGGGG91400.025.8674984
TAACGCA8150.024.7995324
GTATCAA333850.023.2055241
GGTATCA250000.022.8228531
TATCACG5600.022.6634082
GTACATG235450.022.1155681
TACATGG237550.021.4893992
ACATGGG237200.020.5691013
TCAACGC374200.020.4872114
GAGTACT194250.020.38344612-13
CAACGCA376400.020.3426485
ATCAACG378350.020.250073
CATGGGG146200.020.1904014
TGGGGGG82700.020.0606336
TATCAAC384150.020.0307562
Produced by FastQC (version 0.11.5)