FastQCFastQC Report
Mon 6 Jun 2016
ERR522967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4638708
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3849118.297806199484857No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2463195.310077719916839No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1739853.750721105963126No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1601873.453267590889532No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1195752.5777651880652974No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1038812.2394382228844756No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1006932.170712189687301No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT983852.1209569561179533No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA983022.11916766478942No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT930802.006593215179744RNA PCR Primer, Index 26 (100% over 22bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT925641.9954694281252454No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG893711.9266356062938215No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC839401.8095555917725368No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA714731.5407954111360318No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC659281.4212578157538693No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC657561.4175498867357033No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA617821.3318794802345826No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT528461.1392396331047352RNA PCR Primer, Index 26 (100% over 24bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG513751.1075282169086735No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC480081.034943350605384No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT465521.0035553003120696No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG406070.8753946141899857No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC376960.8126400713302065No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG327340.7056706307014798No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT326140.7030837034795033No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT211000.4548680365308616RNA PCR Primer, Index 26 (100% over 27bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT197540.4258513361910256No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG196650.42393269850139303No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG176330.38012739754259156No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA172970.37288400132105753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160730.34649734365689755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155170.33451124752840666No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT148050.31916214601134624No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG141620.3053005276469224RNA PCR Primer, Index 26 (96% over 27bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA130460.2812421044825413No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA129130.2783749268115174No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA128150.27626226958023653No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA121950.26289647893335816No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC111070.23944167212077155No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC110130.23741524579689No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT104290.22482553331660454No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC101770.21939298615045394RNA PCR Primer, Index 26 (95% over 22bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC99770.21508144078049318No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT90860.19587350615731794No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT87540.18871634084318306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84980.18319756276963325No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC83670.18037350055230897No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT77630.16735263353502744No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT76490.16489505267414978RNA PCR Primer, Index 26 (100% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC75580.16293329953081764No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC75010.1617045091003788No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTTATACACATCT68390.14743329392580865No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG67220.14491103988438161No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACATCT65780.14180672721800985No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG62510.134757350538124No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG61380.13232132740409613No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60740.13094163288570868No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC56730.12229698441893733RNA PCR Primer, Index 26 (96% over 28bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT55240.11908488311831657No Hit
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC52770.113760124586415RNA PCR Primer, Index 26 (95% over 22bp)
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA52750.1137170091327154No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA50890.10970727193865189No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG50360.10856471241561227No Hit
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG47770.10298126116151307No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA46540.1003296607589872No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46530.1003081030321374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA13500.059.2256471
GTACCCA12450.056.2874571
CTATACA10100.050.7574541
ACCCATG15600.045.496233
CGGAGAT1550.042.480641
TACCCAT17150.041.1129152
GAGATGT4700.041.0280341
CTTAATA3100.040.9634741
GACTTAT1500.037.6257061
GCGTCAG959.408468E-734.6552541
AGCGTCA550.002717081734.1838043
CGTTATA1151.1373595E-732.6996652
GATGTGT5900.032.6629143
TTACACA24650.032.0343133
GCTTATA6550.031.5941071
ATGGGCG1052.0660427E-631.335495
GACCCGT300.00416196231.33413984-85
CGGTCTA753.2773986E-431.3327877
ATGTGTA6250.030.8337884
CTTATAA5600.030.2349431
Produced by FastQC (version 0.11.5)