FastQCFastQC Report
Mon 6 Jun 2016
ERR522966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4732750
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT263380.5565052031060166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182100.38476572817072524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142710.30153716126987484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82800.17495113834451428No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT71460.1509904389625482No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC49560.10471713063229623No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG36500.031.4282822
GTACATG167850.028.347521
TACATGG172250.027.2119462
ACATGGG174550.025.8315263
GTACCTG47350.025.0228311
CATGGGG122350.025.0150094
ATGGGGG78950.024.177245
ACCTGGG48100.022.9706923
TATCACG5050.021.4121422
GAGTACT150600.020.62221712-13
AGAGTAC222900.019.97102410-11
TATAACG3900.019.2876382
AGTACTT158350.018.63375512-13
GTACTTT168600.018.18406514-15
TGGGGGG83950.018.133666
GAGTACA129850.016.981921
GGGTTAG15900.016.2637791
GTATATA24250.016.0924531
AGTACAT128650.015.823472
GTACAGG29000.015.5642831
Produced by FastQC (version 0.11.5)