FastQCFastQC Report
Mon 6 Jun 2016
ERR522966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4732750
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT266220.5625059426337753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162630.3436268554223232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135300.28588030214991283No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT129430.27347736516824256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80570.17023929005335164No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT71000.15001848819396757No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC56540.11946542707728065No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC52170.11023189477576462No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG40950.029.3830852
GTACATG168200.027.9297181
TACATGG171200.026.9874042
ACATGGG173450.025.9026663
GTACCTG48750.024.6940561
CATGGGG117550.024.547464
ATGGGGG72150.023.3835165
ACCTGGG53650.021.7241523
GAGTACT153350.020.9101212-13
TATAACG3600.020.8895362
TGGGGGG71950.019.3337976
AGAGTAC226950.019.29783610-11
GTACTTT174450.018.12084814-15
AGTACTT160850.018.10823612-13
GAGTACA130350.017.965751
TAACGCA4800.017.6234994
ATAACGC5500.017.0894533
AGTACAT127400.016.8969142
GTATATA25550.016.5645121
CATGGGA61950.016.3101524
Produced by FastQC (version 0.11.5)