FastQCFastQC Report
Mon 6 Jun 2016
ERR522965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3461535
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102950.2974114085225196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88280.25503136614247723No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86960.25121802899580675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46330.13384235606457828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG35000.029.6939352
GTACATG133000.025.2808481
TACATGG136700.024.115322
GTACCTG44400.023.7247851
ACATGGG137600.023.1373583
ACCTGGG43900.022.6027583
CATGGGG91900.022.5656434
GAGTACT90800.020.73241412-13
GTATCAA206100.020.1930771
ATGGGGG51500.019.8130845
GTACTTT96050.019.18337414-15
GGTATCA161700.019.1360841
TCAACGC223350.018.3803164
CAACGCA224850.018.152085
GTATAGG8050.018.1093581
AGAGTAC161000.018.01157610-11
ATCAACG227250.017.9414983
AACGCAG231000.017.7840026
TATCAAC230800.017.6859112
AGTACTT95600.017.528112-13
Produced by FastQC (version 0.11.5)