FastQCFastQC Report
Mon 6 Jun 2016
ERR522965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3461535
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100430.29013140124251235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87490.25274914163803053No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86910.25107358440691774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45800.13231124342235454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG35850.029.8949172
GTACATG128750.023.4187491
GTACCTG46900.023.0678941
TACATGG132650.022.466412
ACCTGGG46450.022.363483
TATAACG3450.021.79982
ACATGGG134650.021.5034033
TAACGCA4500.020.8905724
ATAACGC4550.020.6610053
GAGTACT95850.020.39782512-13
CATGGGG91200.020.2549214
GTACTTT99050.019.45385714-15
GTATCAA218850.018.8497661
GGTATCA173050.018.4293921
ATGGGGG48500.018.1233715
AGAGTAC163450.017.77020510-11
AGTACTT100500.017.58346412-13
GTATAGG9650.017.548021
TCAACGC235650.017.2536724
CAACGCA237650.017.168055
Produced by FastQC (version 0.11.5)