FastQCFastQC Report
Mon 6 Jun 2016
ERR522964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294805
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140870.2660532351994077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117010.22099019699497904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71760.13552907047568324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72150.050.472921
GTATCAA127000.040.2327651
TACCTGG50200.032.5853162
TCAACGC156100.031.762434
ATCAACG159600.031.0093423
CAACGCA161450.030.6499565
TATCAAC164150.030.2677762
AACGCAG168150.029.5896556
GTACATG161250.028.7402341
TACATGG165000.027.263052
GTACCTG62350.026.9392551
ACGCAGA193500.025.7134257
ACATGGG171650.025.3276563
ACCTGGG64250.024.7252463
CGCAGAG203850.024.3843778
CATGGGG124850.023.5640534
ATGGGGG76500.022.0532445
GCAGAGT225400.021.675189
GTGGTAT41250.020.1884561
AGAGTAC203950.019.37060710-11
Produced by FastQC (version 0.11.5)