##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522964_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5294805 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.567760852382666 33.0 31.0 34.0 29.0 34.0 2 31.944745085040903 34.0 31.0 34.0 29.0 35.0 3 31.860486646817023 34.0 31.0 34.0 28.0 35.0 4 34.36011770027414 35.0 34.0 37.0 31.0 37.0 5 34.45348544469532 35.0 34.0 37.0 32.0 37.0 6 34.56489483559829 36.0 34.0 37.0 32.0 37.0 7 34.572797487348446 36.0 35.0 37.0 32.0 37.0 8 34.754396054245625 36.0 35.0 37.0 32.0 37.0 9 35.84963261158815 38.0 35.0 39.0 32.0 39.0 10-11 35.7746172899663 37.5 35.0 39.0 32.0 39.0 12-13 35.731666888582296 37.0 35.0 39.0 31.0 39.0 14-15 36.745981768922555 38.0 36.0 41.0 31.5 41.0 16-17 36.76707716337051 38.0 36.0 41.0 31.5 41.0 18-19 36.635338411896186 38.5 35.0 41.0 31.0 41.0 20-21 36.63388906673617 38.0 36.0 41.0 31.0 41.0 22-23 36.64938066652124 38.5 36.0 41.0 31.0 41.0 24-25 36.55304095240523 38.0 36.0 41.0 30.5 41.0 26-27 36.41922695925535 38.0 35.5 41.0 30.0 41.0 28-29 36.25826324104476 38.0 35.5 40.5 30.0 41.0 30-31 36.20847113349784 38.0 35.5 40.0 29.5 41.0 32-33 35.99121176322829 38.0 35.0 40.0 29.5 41.0 34-35 35.915188283610064 38.0 35.0 40.0 29.0 41.0 36-37 35.73332030169195 38.0 35.0 40.0 29.0 41.0 38-39 35.60559095188586 37.5 35.0 40.0 28.0 41.0 40-41 35.395943004511025 37.0 34.5 40.0 27.5 41.0 42-43 35.318939318822885 37.0 35.0 40.0 26.5 41.0 44-45 35.273289006866165 37.0 34.5 40.0 26.5 41.0 46-47 35.09609409600543 37.0 35.0 40.0 26.0 41.0 48-49 34.97262552634139 36.5 34.5 40.0 25.5 41.0 50-51 34.164433345515086 36.5 33.5 39.0 24.5 40.0 52-53 34.36956167413153 37.0 33.5 39.0 24.5 40.0 54-55 34.992269025960354 37.0 34.5 40.0 26.0 41.0 56-57 34.97986771939665 37.0 34.5 40.0 26.0 41.0 58-59 34.89482643836742 36.5 34.0 40.0 25.0 41.0 60-61 34.623738929006834 37.0 34.0 40.0 25.5 41.0 62-63 34.508962369719 37.0 34.0 39.5 24.5 41.0 64-65 34.313578498169434 37.0 34.0 39.0 25.0 41.0 66-67 33.96750352468126 36.0 34.0 39.0 24.5 41.0 68-69 33.64595240051334 36.0 33.0 38.5 23.5 40.5 70-71 33.42011868236885 36.0 33.0 37.5 22.0 40.0 72-73 33.10220215853086 35.5 33.0 37.5 22.0 39.5 74-75 32.72974868762873 35.0 32.0 37.0 20.5 39.0 76-77 32.3439017112056 35.0 32.0 36.5 20.0 39.0 78-79 32.10781737948801 35.0 31.5 36.5 20.0 38.0 80-81 31.945797815028126 35.0 31.5 37.0 19.5 37.5 82-83 31.666691313466693 35.0 31.0 36.5 18.5 37.5 84-85 31.344570479932692 35.0 31.0 35.5 18.5 37.5 86-87 31.165437726224102 35.0 30.5 36.0 18.5 37.0 88-89 30.879769887654028 35.0 30.0 35.0 18.0 37.0 90-91 30.683883542453405 34.0 30.0 35.0 18.0 37.0 92-93 30.47420008857739 34.0 29.0 35.0 17.5 36.5 94-95 30.220832400815517 34.0 29.0 35.0 17.0 36.0 96-97 29.966330393659447 34.0 29.0 35.0 17.0 36.0 98-99 29.79084678283714 34.0 29.0 35.0 15.0 36.0 100 28.665867581525664 33.0 25.0 35.0 10.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1894993919035315 1101 2 0.09240054592315161 1101 3 0.029847181653785526 1101 4 0.20598157225089864 1101 5 0.07325889246644124 1101 6 0.16459071108297252 1101 7 0.17087647516854076 1101 8 0.11759087734091622 1101 9 0.13912408859855674 1101 10-11 0.21078636471629153 1101 12-13 0.15195432719676916 1101 14-15 0.2113408833186199 1101 16-17 0.1626494679706596 1101 18-19 0.12301741848381198 1101 20-21 0.1245135152615191 1101 22-23 0.07524326633907918 1101 24-25 0.015167917866556024 1101 26-27 0.027721919116729055 1101 28-29 -0.017973218487988163 1101 30-31 -0.0771896554333793 1101 32-33 -0.03611916914800872 1101 34-35 -0.023998576418044593 1101 36-37 -0.085944676432959 1101 38-39 -0.18994192917019603 1101 40-41 0.0020332068703368122 1101 42-43 0.02402903656317079 1101 44-45 -0.12396851459064351 1101 46-47 -0.24148175327315613 1101 48-49 -0.06179231825903031 1101 50-51 -0.20919544993560635 1101 52-53 -0.0765531787097018 1101 54-55 -0.01835411321394531 1101 56-57 -0.00923511071070493 1101 58-59 -0.10845739544062383 1101 60-61 0.27523366644796 1101 62-63 -0.03976971169902299 1101 64-65 -0.08165168251131405 1101 66-67 -0.10460189607392323 1101 68-69 -0.188429770197871 1101 70-71 -0.21293451128658347 1101 72-73 -0.2092860185648462 1101 74-75 -0.2277472243402201 1101 76-77 -0.8284111545735087 1101 78-79 -1.4110482195833178 1101 80-81 -0.37399081017939295 1101 82-83 -0.4629703430247787 1101 84-85 -0.4449763495888668 1101 86-87 -0.7925116879011043 1101 88-89 -0.48810849630319453 1101 90-91 -0.6213670615442091 1101 92-93 -0.6285103441381601 1101 94-95 -1.3086351287084739 1101 96-97 -0.8154883564097837 1101 98-99 -0.6916609935338514 1101 100 -0.9216878380490137 1102 1 0.2402190665971169 1102 2 0.1569731555642413 1102 3 0.04247936162305521 1102 4 0.14718581921933094 1102 5 0.14121706813517676 1102 6 0.30428506672825506 1102 7 0.10759063285101433 1102 8 0.15374935726482875 1102 9 0.14340466538057228 1102 10-11 0.17179030076392365 1102 12-13 0.17391922499012225 1102 14-15 0.22285797350958347 1102 16-17 -0.2725156340935939 1102 18-19 0.12164081680389671 1102 20-21 0.1407527226407268 1102 22-23 -0.0513821219710664 1102 24-25 0.1097225545767273 1102 26-27 -0.04786748061101065 1102 28-29 -0.004778058878237346 1102 30-31 0.05399476965249761 1102 32-33 -0.08550785998723853 1102 34-35 -0.5759628849101333 1102 36-37 -0.5491172108131579 1102 38-39 -0.12282129223400773 1102 40-41 -0.41304136170509764 1102 42-43 -0.2159387344146637 1102 44-45 -0.6126203182918246 1102 46-47 -0.23458323594906005 1102 48-49 -0.09092044553235468 1102 50-51 -0.25906431146920283 1102 52-53 -0.15423067806662516 1102 54-55 -0.1897443173000255 1102 56-57 -0.14049158885853785 1102 58-59 -0.04677563796551709 1102 60-61 -0.07186030574247582 1102 62-63 -0.1529283353882036 1102 64-65 -0.2595164554455067 1102 66-67 -0.21555484392849422 1102 68-69 -0.7579656522818681 1102 70-71 -0.5262247657889887 1102 72-73 -0.29950557948844647 1102 74-75 -0.3085789245970503 1102 76-77 -0.2856138513312061 1102 78-79 -0.29860219215551 1102 80-81 -0.2658162132447899 1102 82-83 -0.9261910516466614 1102 84-85 -0.31786389772902623 1102 86-87 -0.4873022647770462 1102 88-89 -0.32382645460005577 1102 90-91 -0.4719029545355866 1102 92-93 -0.5092365079626937 1102 94-95 -0.8382446764795937 1102 96-97 -0.6892278349584622 1102 98-99 -1.2253509980452648 1102 100 -0.7330521265139502 1103 1 0.08681850972193317 1103 2 0.03662809915385523 1103 3 -0.05773618478127318 1103 4 -0.011355517346061106 1103 5 0.0558713978563361 1103 6 0.16077885990038965 1103 7 0.06825262082845285 1103 8 0.0969998409405406 1103 9 0.10160531895170521 1103 10-11 0.08837541382116854 1103 12-13 0.04361005142130381 1103 14-15 0.06842266846960143 1103 16-17 -0.007250980265936846 1103 18-19 -0.09091319811417264 1103 20-21 -0.010085395376329132 1103 22-23 0.010812756570523163 1103 24-25 -0.14890341707215526 1103 26-27 -0.02824440741418499 1103 28-29 -0.1424451056618139 1103 30-31 -0.1577297109548752 1103 32-33 -0.09312397230026903 1103 34-35 -0.16906364179556732 1103 36-37 -0.19419692916306275 1103 38-39 -0.21482944165970963 1103 40-41 -0.07502209739685384 1103 42-43 -0.08136223253761443 1103 44-45 -0.0372533979737355 1103 46-47 -0.06283827763071059 1103 48-49 -0.08288481600250464 1103 50-51 -0.1346119356974924 1103 52-53 0.0027966673933406128 1103 54-55 -0.042558613936122924 1103 56-57 0.1492498424318498 1103 58-59 0.12966842831556136 1103 60-61 -0.06856256856723064 1103 62-63 -0.018819317949898107 1103 64-65 0.0074108505469538954 1103 66-67 0.06226411971298518 1103 68-69 0.0355434083157391 1103 70-71 -0.002019572390935309 1103 72-73 -0.007527139877488764 1103 74-75 -0.038174442957917165 1103 76-77 -0.02619184776983019 1103 78-79 -0.24480964042360753 1103 80-81 -0.6281933821611219 1103 82-83 -0.33585800346633476 1103 84-85 -0.272043815574051 1103 86-87 -0.33729556616383505 1103 88-89 -0.2607744372648142 1103 90-91 -0.2647408286477173 1103 92-93 -0.2176940101425906 1103 94-95 -0.26040339408170254 1103 96-97 -0.30808784857518035 1103 98-99 -0.3191356144681876 1103 100 -0.3367801240705717 1104 1 0.23902847992972553 1104 2 0.18564734978945907 1104 3 0.17696710916271385 1104 4 0.08542807716897727 1104 5 0.1391839623853528 1104 6 0.11338253397313025 1104 7 0.08869367499827518 1104 8 -0.3612848825032131 1104 9 0.04243659494704133 1104 10-11 0.10930534075009035 1104 12-13 0.12107504303420313 1104 14-15 0.055179119738532734 1104 16-17 0.07175065208684117 1104 18-19 0.09131136120764438 1104 20-21 0.10162691109716349 1104 22-23 0.10191954049570029 1104 24-25 0.09983701351241336 1104 26-27 0.08508692996090161 1104 28-29 0.18819125576257534 1104 30-31 0.16652082574638172 1104 32-33 0.04031926229124139 1104 34-35 -0.056438804644585616 1104 36-37 -0.0424180852023639 1104 38-39 -0.009844554290729945 1104 40-41 -0.037510113665263134 1104 42-43 0.05415391283298021 1104 44-45 0.013792059779582644 1104 46-47 -0.053274633175853126 1104 48-49 -0.17324553774619034 1104 50-51 -0.20328890640686126 1104 52-53 -0.042837371509271804 1104 54-55 -0.11838831681150452 1104 56-57 -0.10544723169716264 1104 58-59 -0.046934167675495075 1104 60-61 0.004774657594197151 1104 62-63 0.043528987357873916 1104 64-65 -0.14814102242731053 1104 66-67 -0.02470333844849648 1104 68-69 -0.12579928194654144 1104 70-71 -0.05246101300548389 1104 72-73 0.021642739057419647 1104 74-75 -0.12166336647228349 1104 76-77 -0.17101791626655682 1104 78-79 -0.11786649414921513 1104 80-81 -0.047486585405831505 1104 82-83 -0.052324244290240074 1104 84-85 -0.10869546619029435 1104 86-87 -0.17078783849880708 1104 88-89 -0.2337162420291996 1104 90-91 -0.21452830056487926 1104 92-93 -0.24307234521468146 1104 94-95 -0.24956585826904742 1104 96-97 -0.33591869471039715 1104 98-99 -0.3043758849149043 1104 100 -0.23520435809621176 1105 1 0.12434286634674407 1105 2 0.08640580684868482 1105 3 0.08506911532296257 1105 4 0.03915576320686398 1105 5 0.11901488076888 1105 6 0.0613781607618904 1105 7 0.027902043117826736 1105 8 0.04702594174446517 1105 9 0.04519197462920488 1105 10-11 0.026141049321196874 1105 12-13 0.061951518145299644 1105 14-15 0.023937749086890392 1105 16-17 -0.012915560640742285 1105 18-19 -0.06398816985434053 1105 20-21 -0.05367393962622202 1105 22-23 0.02765592530163019 1105 24-25 -0.0718658353982704 1105 26-27 -0.0859269457288292 1105 28-29 -0.050721214945980364 1105 30-31 0.01686094097848212 1105 32-33 0.007760502992894658 1105 34-35 -0.0017827184853587141 1105 36-37 0.05495369915396253 1105 38-39 -0.08206804627973696 1105 40-41 -0.06391201104509037 1105 42-43 -0.029192015324291276 1105 44-45 -0.02423425830097159 1105 46-47 -0.07321237081800547 1105 48-49 -0.15892691833195727 1105 50-51 -0.10172913995026533 1105 52-53 0.06628643747340845 1105 54-55 0.08122691024814799 1105 56-57 0.07014548413850008 1105 58-59 0.11462030180687321 1105 60-61 0.11510843112014157 1105 62-63 0.1935369511478342 1105 64-65 0.21833736032765216 1105 66-67 0.1515136322137849 1105 68-69 0.16461315806155596 1105 70-71 -0.04471130145230262 1105 72-73 0.10757100629619032 1105 74-75 0.10188545962474649 1105 76-77 0.029231939624587255 1105 78-79 0.11008232131721485 1105 80-81 0.024555049911242577 1105 82-83 0.05236960801215673 1105 84-85 -0.056249519612755705 1105 86-87 -0.12427919517487496 1105 88-89 -0.07966199478147118 1105 90-91 -0.07756784733449962 1105 92-93 -0.09463197420654268 1105 94-95 -0.10676735131928794 1105 96-97 -0.21821503384137841 1105 98-99 -0.06484247686185185 1105 100 -0.07879328723680956 1106 1 0.2881421389592411 1106 2 0.20111951458811816 1106 3 0.2201077084665819 1106 4 0.20368722953111984 1106 5 0.20936307987506808 1106 6 0.254446638484751 1106 7 0.15357505589373233 1106 8 0.1101715698873349 1106 9 0.24434995544740445 1106 10-11 0.19690168574939548 1106 12-13 0.11192268088928614 1106 14-15 0.14803150962288925 1106 16-17 0.22641657353140943 1106 18-19 0.1821047585618345 1106 20-21 0.11095512144132869 1106 22-23 0.1261816385215795 1106 24-25 0.06776948450158926 1106 26-27 0.1677582870136547 1106 28-29 0.22018943618778763 1106 30-31 0.157366812444657 1106 32-33 0.16280124817054542 1106 34-35 0.18515020261390447 1106 36-37 0.14816338617648483 1106 38-39 0.19038545185157574 1106 40-41 0.1606373709624762 1106 42-43 0.24247453776280992 1106 44-45 0.20210896960660563 1106 46-47 0.18538315797262328 1106 48-49 0.24203278124409167 1106 50-51 0.14000310397562288 1106 52-53 0.19700842695713305 1106 54-55 0.21895705235412777 1106 56-57 0.2579463743632431 1106 58-59 0.21831798047950457 1106 60-61 0.2613247966216079 1106 62-63 0.27300964900943114 1106 64-65 0.33364300296088345 1106 66-67 0.3287858035443847 1106 68-69 0.30893998388309996 1106 70-71 0.2936131965857598 1106 72-73 0.23320793663484807 1106 74-75 0.18340601513646249 1106 76-77 0.2477476458862924 1106 78-79 0.29750759193549214 1106 80-81 0.2278140902475343 1106 82-83 0.1863104306630312 1106 84-85 0.13757787291107704 1106 86-87 0.06688462482900093 1106 88-89 0.1430100424056775 1106 90-91 0.20027737479127694 1106 92-93 0.31570801130309434 1106 94-95 0.19309029162957003 1106 96-97 0.13150480719503932 1106 98-99 0.10806492111380095 1106 100 0.26416324884921494 1107 1 0.1952973275986878 1107 2 0.05721662836888797 1107 3 0.03094011693767129 1107 4 0.17562658452894908 1107 5 0.2282448727448667 1107 6 0.22092524982842576 1107 7 0.22051480030724235 1107 8 0.16831295374085897 1107 9 0.36544200682431693 1107 10-11 0.17673076471641025 1107 12-13 0.24545494073338858 1107 14-15 0.23343401940695685 1107 16-17 0.2792111989459727 1107 18-19 0.2598374019803771 1107 20-21 0.21328139337030194 1107 22-23 0.25708047481771246 1107 24-25 0.25701942122153554 1107 26-27 0.2922029640257904 1107 28-29 0.25071597486649466 1107 30-31 0.10396169682065448 1107 32-33 0.19159934912007515 1107 34-35 0.2016630500862675 1107 36-37 0.1500634101904481 1107 38-39 0.1045996110057672 1107 40-41 0.0651911817194275 1107 42-43 0.151350051304739 1107 44-45 0.1239345827740479 1107 46-47 0.1534790613196506 1107 48-49 0.16210926897425537 1107 50-51 0.010421790736131697 1107 52-53 0.1118687727582568 1107 54-55 0.224310706695789 1107 56-57 0.235669769159351 1107 58-59 0.26528272862490354 1107 60-61 0.2947918293390046 1107 62-63 0.1590394425542101 1107 64-65 0.2761088641861136 1107 66-67 0.22946087185437847 1107 68-69 0.2953968366255566 1107 70-71 0.2969313427075022 1107 72-73 0.24174993187099147 1107 74-75 0.24347500661475152 1107 76-77 0.2513473070946475 1107 78-79 0.20572637215577316 1107 80-81 0.18968704987161544 1107 82-83 -0.6250545848811448 1107 84-85 0.22166245399715834 1107 86-87 0.18177685403906807 1107 88-89 0.1491125106520741 1107 90-91 0.07687438472786567 1107 92-93 0.06604144848060756 1107 94-95 0.03724207937206003 1107 96-97 0.17293457932196432 1107 98-99 0.0891733928519578 1107 100 0.05817046601985254 1108 1 0.008423721046217025 1108 2 0.08192338612058947 1108 3 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-2.025960391996623 2316 100 -0.2635959474526146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 7487.0 1 604.0 2 323.0 3 226.0 4 203.0 5 224.0 6 272.0 7 807.0 8 1509.0 9 2186.0 10 3771.0 11 7180.0 12 11811.0 13 15477.0 14 17373.0 15 18983.0 16 21332.0 17 25188.0 18 29907.0 19 34909.0 20 41008.0 21 47252.0 22 53449.0 23 58508.0 24 61075.0 25 63829.0 26 68757.0 27 77173.0 28 88008.0 29 103239.0 30 124264.0 31 152962.0 32 196459.0 33 266329.0 34 406173.0 35 741672.0 36 744114.0 37 789583.0 38 813899.0 39 197088.0 40 192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.16969891621337 18.12602778903215 12.670494288264017 24.033779006490462 2 15.991014598156225 20.092340083042885 40.04363289339702 23.87301242540387 3 18.868135963911854 25.520576704626656 29.778397730161583 25.832889601299907 4 12.973879435248426 16.187425296471865 37.362543142322586 33.47615212595713 5 13.530082867181381 37.13567316973168 34.53015650250029 14.804087460586649 6 31.407364180024054 36.04170971533759 18.259751328169763 14.291174776468592 7 27.594218795745835 32.068465434293394 22.35408693229744 17.983228837663333 8 26.013771982461147 34.89219503767341 20.597362435819342 18.496670544046104 9 26.913661642291647 15.542490195618324 20.120631174338218 37.42321698775181 10-11 25.0473597691498 25.53647835188098 28.76353702236361 20.652624856605602 12-13 25.4531290151301 23.454102769921764 28.549682498413343 22.543085716534794 14-15 22.999546117850812 24.962912776867636 26.073457770324282 25.964083334957266 16-17 22.735287445250034 27.674539082332938 26.679044714420353 22.91112875799668 18-19 22.565070196847884 26.895119988328435 28.381380450017495 22.15842936480619 20-21 23.310219474280096 26.252995667203948 28.248520863448473 22.188263995067476 22-23 23.11531543141419 26.035880478892093 28.096131154204574 22.752672935489148 24-25 22.828482573556098 26.10821844558974 28.202602679955056 22.860696300899107 26-27 22.67405613786556 26.28852035310578 28.208362952941084 22.829060556087576 28-29 22.720684882617665 26.439788544895038 28.22093220848946 22.61859436399783 30-31 23.152937966342364 26.18648131300211 28.16833700932344 22.49224371133209 32-33 22.904037498410315 26.36373253616913 28.063579472163852 22.668650493256706 34-35 23.081413556342525 26.457433129883807 27.967125760570404 22.494027553203267 36-37 23.093213884201685 26.338253463301314 27.896022334075948 22.67251031842105 38-39 22.921760136691418 26.52391513110256 27.881858370399982 22.672466361806045 40-41 23.013928261507992 26.317067148233246 27.981403872830306 22.687600717428456 42-43 22.856792997165932 26.39613766481795 27.8393371067291 22.907732231287024 44-45 22.71027627981022 26.531420514146557 27.864997193291792 22.893306012751435 46-47 22.790851033634514 26.382194048914332 27.658640427645587 23.16831448980557 48-49 22.83865878966179 26.509431248606706 27.591904382880266 23.060005578851232 50-51 22.7733691803857 26.7111575369888 27.56993113365136 22.94554214897414 52-53 22.811080753947035 26.73082364653788 27.424364447481214 23.033731152033873 54-55 22.656413448397537 26.57868767498958 27.63921598610312 23.125682890509758 56-57 22.741734924575677 26.744699410462523 27.37942722127525 23.13413844368655 58-59 22.71681967836786 26.6336321643498 27.546980002328063 23.102568154954277 60-61 22.8358540986301 26.62844680617323 27.613349276832004 22.922349818364665 62-63 22.717644221065203 26.653550448972275 27.607695196790104 23.021110133172414 64-65 22.653371269351975 26.404029197334484 27.624136649914938 23.3184628833986 66-67 22.586822349055566 26.501135841236973 27.496196356034325 23.41584545367314 68-69 22.471834513280953 26.533978431718975 27.57444514797899 23.41974190702108 70-71 22.525006295996146 26.330135065087966 27.58959928882926 23.55525935008663 72-73 22.444549619476735 26.584699557002768 27.353962364166446 23.61678845935406 74-75 22.44846366816853 26.581516391565195 27.405155015031863 23.564864925234414 76-77 22.41590466531901 26.439692134540415 27.393904266929837 23.750498933210736 78-79 22.34860715342835 26.362873180788927 27.49725074268296 23.791268923099764 80-81 22.442108364575144 26.528838501400365 27.234020757351146 23.795032376673344 82-83 22.50300358157576 26.455366635770883 27.343555767207988 23.698074015445368 84-85 22.482210713088115 26.466119173879548 27.221400577005113 23.830269536027224 86-87 22.34555547341052 26.42507254137672 27.437021551838793 23.792350433373965 88-89 22.249905291717603 26.248951866609843 27.530519164204375 23.970623677468176 90-91 22.591281151977512 26.170477050379944 27.42577066101876 23.812471136623785 92-93 22.504461705997354 26.119099035911823 27.413365288187634 23.963073969903185 94-95 22.406240270167597 25.924623399574216 27.476019587037158 24.193116743221033 96-97 22.371234837310773 25.840580644568796 27.539962581634324 24.248221936486107 98-99 22.400624548048107 25.807492115043022 27.429842013050298 24.36204132385857 100 22.50633337555899 25.67901416907571 27.543646278489774 24.271006176875527 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3999.0 1 4488.0 2 3700.5 3 2139.0 4 1461.5 5 1216.5 6 1612.0 7 2731.5 8 4324.5 9 5210.5 10 4757.0 11 4035.5 12 4157.5 13 4527.0 14 4788.0 15 5090.0 16 5499.0 17 5900.5 18 6384.0 19 6959.0 20 7635.0 21 8609.0 22 10021.5 23 12163.5 24 14859.0 25 18173.5 26 22684.5 27 28449.0 28 34675.0 29 41738.0 30 50346.0 31 58780.0 32 67841.0 33 78138.5 34 88063.5 35 98375.5 36 111296.0 37 123518.5 38 133323.0 39 142404.5 40 149738.5 41 155768.0 42 163048.5 43 171805.0 44 179607.0 45 188697.5 46 197271.0 47 203739.0 48 210878.0 49 215646.0 50 216054.0 51 212941.0 52 206907.0 53 201362.5 54 193480.5 55 180544.5 56 164695.0 57 147618.0 58 131742.5 59 114933.0 60 96352.5 61 79527.0 62 65794.5 63 52040.5 64 39172.0 65 29650.5 66 22260.0 67 16980.5 68 13030.0 69 9878.5 70 7710.0 71 6059.5 72 4655.5 73 3611.5 74 2827.5 75 2022.0 76 1416.0 77 970.0 78 645.5 79 465.0 80 317.0 81 213.0 82 156.5 83 114.0 84 81.5 85 62.5 86 45.5 87 30.5 88 30.0 89 30.0 90 20.5 91 15.5 92 15.0 93 13.5 94 11.0 95 10.5 96 8.0 97 4.0 98 2.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15054378773155952 2 0.176455979020946 3 0.22591955699973842 4 0.21568310825422274 5 0.22690165171333032 6 0.19054526087362988 7 0.20499338502551084 8 0.19468139053279582 9 0.1970421951327764 10-11 0.18872271972244492 12-13 0.1908852167360271 14-15 0.17549277074415395 16-17 0.18210302362409947 18-19 0.19199951650721794 20-21 0.18660743880086236 22-23 0.1964094994999816 24-25 0.1849171027072763 26-27 0.1971649569719754 28-29 0.19413368386560034 30-31 0.19001644064323425 32-33 0.20285921766712844 34-35 0.19113074041442507 36-37 0.19498357352159332 38-39 0.20924283330547586 40-41 0.22228391791576838 42-43 0.20885566135107905 44-45 0.19159345811602127 46-47 0.1899031220224352 48-49 0.19949743191675615 50-51 0.20538055697990765 52-53 0.21549424388622435 54-55 0.20284977444872854 56-57 0.21899767791259545 58-59 0.21606083699021966 60-61 0.20262313720713038 62-63 0.2080435445686857 64-65 0.2109992719278614 66-67 0.20880844525907943 68-69 0.21089539652546221 70-71 0.22069745722458145 72-73 0.23806353586203838 74-75 0.2271471753917283 76-77 0.2556656949594933 78-79 0.26984940899617643 80-81 0.2585175469162698 82-83 0.257044404845882 84-85 0.2530027073707153 86-87 0.24211467655560498 88-89 0.24167084529080865 90-91 0.23372909861647406 92-93 0.23329471057007764 94-95 0.24640189770916962 96-97 0.25890471887066663 98-99 0.2558356728906919 100 0.25791318093867477 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5294805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.43902044446914 #Duplication Level Percentage of deduplicated Percentage of total 1 78.40092906706337 49.73678141950808 2 13.09477317034474 16.614391657383717 3 3.9409491715884197 7.500298652010345 4 1.653044140811981 4.194700041783247 5 0.8451471373374672 2.6807653262068074 6 0.49794647583086193 1.8953541996291245 7 0.32756613166040704 1.454633216632416 8 0.21779365075267376 1.1053292690219556 9 0.16044192905518637 0.9160450935733833 >10 0.8032727860257614 8.835074403039425 >50 0.04017624555600652 1.7410644381753886 >100 0.01680963206664627 1.8875153751757177 >500 7.793452841768681E-4 0.32718102087420414 >1k 2.597816355837466E-4 0.334785370315037 >5k 3.711166222624949E-5 0.16894654130665995 >10k+ 7.422332445249898E-5 0.6071339753645689 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14087 0.2660532351994077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11701 0.22099019699497904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7176 0.13552907047568324 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.7772873599688754E-5 7 0.0 0.0 0.0 0.0 5.6659310399533124E-5 8 0.0 0.0 0.0 0.0 7.554574719937751E-5 9 0.0 0.0 0.0 0.0 1.1331862079906625E-4 10-11 0.0 0.0 0.0 0.0 1.1331862079906625E-4 12-13 0.0 9.443218399922188E-6 0.0 0.0 1.1331862079906625E-4 14-15 0.0 1.8886436799844377E-5 0.0 0.0 2.0775080479828813E-4 16-17 0.0 1.8886436799844377E-5 0.0 1.8886436799844377E-5 3.0218298879751003E-4 18-19 0.0 1.8886436799844377E-5 0.0 1.8886436799844377E-5 3.6828551759696533E-4 20-21 0.0 1.8886436799844377E-5 0.0 4.721609199961094E-5 3.871719543968097E-4 22-23 0.0 1.8886436799844377E-5 0.0 2.7385333359774346E-4 4.3438804639642063E-4 24-25 0.0 1.8886436799844377E-5 0.0 9.726514951919853E-4 4.721609199961094E-4 26-27 0.0 1.8886436799844377E-5 0.0 0.0019453029903839705 4.721609199961094E-4 28-29 0.0 1.8886436799844377E-5 0.0 0.005354304832755881 4.721609199961094E-4 30-31 0.0 1.8886436799844377E-5 0.0 0.016431200015864608 4.8160413839603157E-4 32-33 0.0 1.8886436799844377E-5 0.0 0.04746161567800892 5.477066671954869E-4 34-35 0.0 1.8886436799844377E-5 0.0 0.09777508331279433 6.043659775950201E-4 36-37 0.0 1.8886436799844377E-5 0.0 0.1759460452273502 6.043659775950201E-4 38-39 0.0 1.8886436799844377E-5 0.0 0.28654501912723884 6.421388511947088E-4 40-41 0.0 1.8886436799844377E-5 0.0 0.40251718429668326 6.610252879945531E-4 42-43 0.0 3.7772873599688754E-5 0.0 0.5173278336029372 6.704685063944753E-4 44-45 0.0 3.7772873599688754E-5 0.0 0.6668045376553056 6.799117247943975E-4 46-47 0.0 3.7772873599688754E-5 0.0 0.8466317456450239 6.799117247943975E-4 48-49 0.0 3.7772873599688754E-5 0.0 1.0316149508810994 6.987981615942419E-4 50-51 0.0 3.7772873599688754E-5 0.0 1.222339632904328 6.987981615942419E-4 52-53 0.0 3.7772873599688754E-5 0.0 1.4114589677995695 7.649006903936973E-4 54-55 0.0 3.7772873599688754E-5 0.0 1.6334123730713408 8.49889655992997E-4 56-57 0.0 3.7772873599688754E-5 0.0 1.8936485857363963 8.49889655992997E-4 58-59 0.0 3.7772873599688754E-5 0.0 2.1744049119844826 8.49889655992997E-4 60-61 1.8886436799844377E-5 6.610252879945531E-5 0.0 2.4635562593901 8.49889655992997E-4 62-63 1.8886436799844377E-5 7.554574719937751E-5 0.0 2.7581657870308725 8.687760927928413E-4 64-65 1.8886436799844377E-5 7.554574719937751E-5 0.0 3.062652921117964 8.687760927928413E-4 66-67 1.8886436799844377E-5 7.554574719937751E-5 0.0 3.4068960046687273 8.687760927928413E-4 68-69 1.8886436799844377E-5 7.554574719937751E-5 0.0 3.7962682289527185 8.687760927928413E-4 70-71 1.8886436799844377E-5 7.554574719937751E-5 0.0 4.206160566819742 8.971057479926078E-4 72-73 1.8886436799844377E-5 7.554574719937751E-5 0.0 4.606288616861244 0.001010424368791674 74-75 1.8886436799844377E-5 7.554574719937751E-5 0.0 5.012394224149897 0.001048197242391363 76-77 1.8886436799844377E-5 7.554574719937751E-5 0.0 5.448293185490306 0.0010954133343909738 78-79 1.8886436799844377E-5 7.554574719937751E-5 0.0 5.930312825495934 0.0011331862079906625 80-81 1.8886436799844377E-5 8.498896559929969E-5 0.0 6.43087894643901 0.001161515863190429 82-83 1.8886436799844377E-5 9.443218399922188E-5 0.0 6.945675997510767 0.0012087319551900401 84-85 1.8886436799844377E-5 9.443218399922188E-5 0.0 7.462446681228109 0.0012087319551900401 86-87 1.8886436799844377E-5 9.443218399922188E-5 0.0 7.993391635763734 0.0012087319551900401 88 1.8886436799844377E-5 9.443218399922188E-5 0.0 8.40890268857871 0.0012653912655895732 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7215 0.0 50.47292 1 GTATCAA 12700 0.0 40.232765 1 TACCTGG 5020 0.0 32.585316 2 TCAACGC 15610 0.0 31.76243 4 ATCAACG 15960 0.0 31.009342 3 CAACGCA 16145 0.0 30.649956 5 TATCAAC 16415 0.0 30.267776 2 AACGCAG 16815 0.0 29.589655 6 GTACATG 16125 0.0 28.740234 1 TACATGG 16500 0.0 27.26305 2 GTACCTG 6235 0.0 26.939255 1 ACGCAGA 19350 0.0 25.713425 7 ACATGGG 17165 0.0 25.327656 3 ACCTGGG 6425 0.0 24.725246 3 CGCAGAG 20385 0.0 24.384377 8 CATGGGG 12485 0.0 23.564053 4 ATGGGGG 7650 0.0 22.053244 5 GCAGAGT 22540 0.0 21.67518 9 GTGGTAT 4125 0.0 20.188456 1 AGAGTAC 20395 0.0 19.370607 10-11 >>END_MODULE