FastQCFastQC Report
Mon 6 Jun 2016
ERR522964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294805
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132540.25032083334513733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116960.2208957648109798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69560.13137405437971747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77800.049.641341
GTATCAA135150.040.1322171
TACCTGG52300.035.0700262
TCAACGC164650.032.2514574
CAACGCA167950.031.5338155
ATCAACG169250.031.4038543
TATCAAC174400.030.795892
AACGCAG174850.030.4772626
GTACCTG65050.029.8663851
GTACATG172600.028.4537561
ACCTGGG65200.027.7499473
TACATGG177500.027.2375432
ACGCAGA203700.026.0446857
ACATGGG182200.025.5092073
CGCAGAG211200.025.0378998
CATGGGG125850.024.8313924
ATGGGGG74400.023.3701445
GCAGAGT238950.021.9533399
TATAACG4800.020.5755392
GTGGTAT43400.020.1605831
Produced by FastQC (version 0.11.5)