FastQCFastQC Report
Mon 6 Jun 2016
ERR522963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8395019
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT573760.6834528903389022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260070.3097908414501504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194700.23192323924460445No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT167880.1999757236999702No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC129800.1546154928297363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119090.141857927897483No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC111420.13272155786663498No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG91870.10943393933950596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG255850.025.5081211
TACATGG264250.024.0711842
ACATGGG261050.023.5028023
CATGGGG167450.020.8321174
GAGTACT241750.020.0361712-13
AGAGTAC349350.020.0029510-11
AGTACTT257900.018.10746212-13
GTACTTT266200.018.01148614-15
TACCTGG55450.017.2959042
ATGGGGG94200.017.068185
GAGTACA204500.016.6693671
GTATTAG29800.016.2515741
GTGTAGC52200.015.943261
AGTACAT201350.015.4801992
GTATAGC33100.015.1995311
TATCACG7150.015.1229492
GTACCTG68500.015.0323871
CATGGGA102700.015.014754
TAGCCCT58700.014.896684
TACTTTT317000.014.73974914-15
Produced by FastQC (version 0.11.5)