FastQCFastQC Report
Mon 6 Jun 2016
ERR522963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8395019
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT573850.6835600967669043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238490.2840851223803067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188350.22435923015778758No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC134200.159856695976507No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC117310.13973762298810757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113920.1356995142000274No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA95450.11369837280892396No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG92620.1103273262395237No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA84470.10061918859266428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG254600.025.3238091
TACATGG257650.024.2979972
ACATGGG259650.023.3479423
CATGGGG168150.021.3803274
GAGTACT248300.020.58981312-13
AGAGTAC355100.019.83174910-11
ATGGGGG98800.018.8835375
GTACTTT270850.018.69905914-15
AGTACTT264750.018.5025512-13
TACCTGG57700.018.2460382
GTATAGC35200.017.6352351
ATAACGC7550.016.8059883
GAGTACA199150.016.151981
GTACCTG68450.015.8704231
TATCACG7250.015.5585672
TATAACG7600.015.4604732
TACTTTT314500.015.43123514-15
GTGTAGC56750.015.413351
ACTTTTT336650.015.20235116-17
CATGGGA101950.014.8428894
Produced by FastQC (version 0.11.5)