FastQCFastQC Report
Mon 6 Jun 2016
ERR522962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6688494
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT485080.7252454737942502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241720.3613967508978852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185800.2777904861692333No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT137950.20624971779895446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111080.16607624975069127No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC105020.15701591419533306No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC97820.14625115908005598No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA77940.1165284741228743No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG75400.1127309077349849No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA69390.10374532742348277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG245250.029.6926271
TACATGG254900.028.0690822
TACCTGG51000.027.9290312
ACATGGG260050.026.719023
CATGGGG179600.025.466184
ATGGGGG113450.023.3281765
GTACCTG68250.021.8354851
GAGTACT204600.020.52868312-13
ACCTGGG71500.019.7908043
AGAGTAC310550.019.68834710-11
TATAACG6250.019.5558512
TAACGCA6250.019.5546764
GAGTACA183850.019.1524541
AGTACTT215450.018.5135812-13
GTACTTT226150.018.37561214-15
TGGGGGG113850.017.9094666
AGTACAT183350.017.7678852
TATCACG6350.017.767282
TATACTG26650.016.9336625
ATAACGC7600.016.701413
Produced by FastQC (version 0.11.5)