FastQCFastQC Report
Mon 6 Jun 2016
ERR522962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6688494
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT497870.7443678651726382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223730.33449981415846375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180570.2699710876618862No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT140800.21051076669875163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108780.16263750853331108No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC108310.16193480924106382No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC100820.15073647371142143No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG80690.12064001253495929No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA80090.1197429496086862No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA72470.10835025044501796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG248650.028.4067571
TACATGG251950.027.628882
TACCTGG54150.027.2554742
ACATGGG258100.026.2375853
CATGGGG175500.024.6890814
ATGGGGG111100.023.053155
GTACCTG66600.022.3833561
TATAACG7100.021.8463132
GAGTACT203200.020.13875412-13
ACCTGGG71050.019.9090393
TAACGCA6150.019.867784
TGGGGGG103250.019.844696
AGAGTAC315350.019.57309210-11
AGTACTT213500.018.60571312-13
TATACCG5100.018.429255
GTACTTT222000.018.32489614-15
GTATTAG21000.017.690821
GAGTACA189150.016.5331441
GTATAAC18350.016.401521
GTATTAT21100.016.0468651
Produced by FastQC (version 0.11.5)