FastQCFastQC Report
Mon 6 Jun 2016
ERR522961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4067577
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT154420.3796363289496425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108270.2661781202912692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89380.2197376964222189No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45320.1114176818287644No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43060.10586154853368479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG148350.024.2859211
TACCTGG34950.023.5510122
TACATGG152350.023.4015942
ACATGGG153650.022.7747573
CCGTATC19750.022.36632594
GAGTACT95700.020.9703612-13
CATGGGG95050.020.733374
GTACTTT102850.019.23870314-15
CGTATCA14000.019.13289594
GCCGTAT25850.018.90630594
CGCCGTA30050.018.76595394
AGAGTAC177550.017.52246510-11
ACCTGGG45350.017.42393
AGTACTT102100.017.1010612-13
CATGGGT29050.017.000094
TAACGCA4205.4569682E-1116.7977074
GTATAAT17050.016.5516091
GAGTACA124700.016.0300851
ATGGGGG53300.015.9708225
GTACCTG55700.015.9595261
Produced by FastQC (version 0.11.5)