FastQCFastQC Report
Mon 6 Jun 2016
ERR522961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4067577
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT211620.5202605875684714No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT151870.3733672404972297No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT112370.2762578311363251RNA PCR Primer, Index 26 (100% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101130.24862467262451332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89690.22049982090074755No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT60080.1477046408709657RNA PCR Primer, Index 26 (100% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53600.13177378080365781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45270.11129475852577592No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT42100.10350142111630585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG148800.024.2450941
TACATGG152900.023.6721082
ACATGGG155350.022.2391973
TACCTGG35500.021.4506442
GAGTACT96000.020.8053612-13
CATGGGG91750.020.1339744
GTACTTT101200.019.50412214-15
GTATAGG10350.018.6326641
ATGGGGG47900.018.05665
ACCTGGG43950.017.1121313
AGAGTAC179300.016.93247810-11
TATAACG3351.661283E-816.8379862
AGTACTT102700.016.70244412-13
GAGTACA126100.016.5614871
ACTTTTT123450.016.0838716-17
ATAACGC4158.458301E-1015.857073
AGTACAT116650.015.7156932
CATGGGT29800.015.4579844
GTATAGC12600.015.3054041
GTATATG15750.015.2307421
Produced by FastQC (version 0.11.5)