FastQCFastQC Report
Mon 6 Jun 2016
ERR522960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122985
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT126150.4039404608091297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83240.2665398649048907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70400.22542535426843227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36540.11700344382057551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG39750.040.4622572
ACCTGGG43800.035.5398033
GTACCTG58750.027.5371281
CCTGGGG41000.025.5789184
GTACATG110750.025.1388911
TACATGG110850.024.3521522
ACATGGG111000.023.556623
CATGGGG72350.023.2054964
ATGGGGG43400.020.6948855
GCCGTAT22950.020.26990794
GAGTACT75550.019.84460412-13
CCGTATC18300.019.51469694
TAACGCA3204.5474735E-1019.1053284
CGTATCA13700.018.52134394
TATAACG2352.7831466E-618.0109162
AGAGTAC133700.017.9447210-11
AGTACTT81150.017.92496912-13
TATCACG3451.2387318E-917.7208862
CGCCGTA26600.017.66516194
GTACTTT84500.017.6601414-15
Produced by FastQC (version 0.11.5)