FastQCFastQC Report
Mon 6 Jun 2016
ERR522960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122985
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT126720.4057656376831781No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT122350.3917726149821405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76050.2435170197743505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68740.22010992688085276No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT64670.20707752358720904RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35590.11396148236382819No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT33330.10672481616146091RNA PCR Primer, Index 31 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG39350.043.1223222
ACCTGGG44000.037.1751143
GTACCTG58500.029.7527031
CCTGGGG43050.025.9854094
GTACATG109200.024.8992211
TACATGG111550.024.0184482
ACATGGG113300.022.899973
ATGGGGG40500.022.1668555
CATGGGG72650.021.9325854
TGGGGGG37350.021.014736
GAGTACT79350.020.46309312-13
GGGTTAG8500.019.3700031
TATAACG3657.2759576E-1219.3169292
TAAGGCG5500.018.37140718-19
TACCGGG4101.8189894E-1218.3432272
GTACTTT86850.018.29013814-15
AATCTCG10250.017.65281326-27
GTATACG1600.001271726117.6405371
AGAGTAC137100.017.5507510-11
CGAGACT9800.017.50398312-13
Produced by FastQC (version 0.11.5)