FastQCFastQC Report
Mon 6 Jun 2016
ERR522959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4865943
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220180.45249194246623936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188770.3879412479759833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117420.24130985504762387No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT116690.23980963196650681No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82860.17028559520734213No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50080.1029194135648527No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG64050.043.9061242
GTACCTG73950.039.120591
ACCTGGG73850.037.1889883
CCTGGGG70500.027.4117984
TATCACG5000.025.3943332
TATAACG5300.024.8442122
CATGGGG131950.023.6615664
ATAACGC5850.023.3127823
TAACGCA6100.023.1247084
GTACATG242650.022.7591631
ATGGGGG77350.022.7334185
TACATGG249950.021.7682382
GAGTACT185550.021.53764712-13
ACATGGG247950.021.2804473
GTATAAC12250.021.1200581
GTATCAA368600.019.5383721
TGGGGGG83100.019.4524176
AGTACTT194400.018.949812-13
GGTATCA292200.018.9158631
GTACTTT220400.018.01537914-15
Produced by FastQC (version 0.11.5)