FastQCFastQC Report
Mon 6 Jun 2016
ERR522959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4865943
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191290.39312010025600386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179900.36971250999035543No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118550.24363211817318864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113340.23292504659425728No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71800.1475561879783631No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG65500.047.2857322
GTACCTG79350.039.524451
ACCTGGG78450.038.8171353
CCTGGGG71850.029.0400664
CATGGGG129150.022.9966244
ATGGGGG77950.021.8239755
TATAACG4750.021.7678262
GTACATG246650.021.4465921
TACATGG252950.020.5683292
TGGGGGG75400.020.255786
ACATGGG249050.020.114633
GAGTACT188150.020.02344912-13
CTGGGGG46000.019.6148415
GTATCAA364150.018.5809291
GGTATCA293750.018.5040531
TAACGCA5100.018.4289784
TATCACG4101.8189894E-1218.3409652
AGTACTT196800.018.2596312-13
ATAACGC5450.017.2454643
GTACTTT217800.017.1448314-15
Produced by FastQC (version 0.11.5)