##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522959_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4865943
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.173019289375155	33.0	31.0	34.0	30.0	34.0
2	32.30834516557222	34.0	31.0	34.0	30.0	34.0
3	32.49440098250226	34.0	31.0	34.0	30.0	35.0
4	35.59251454445726	37.0	35.0	37.0	33.0	37.0
5	35.529621699226645	36.0	35.0	37.0	33.0	37.0
6	35.66042676619927	37.0	35.0	37.0	33.0	37.0
7	35.50213350218036	36.0	35.0	37.0	33.0	37.0
8	35.49121865998019	36.0	35.0	37.0	33.0	37.0
9	36.773562082416504	37.0	37.0	39.0	33.0	39.0
10-11	36.57430091145745	37.5	35.0	39.0	33.0	39.0
12-13	36.58109907987003	37.5	35.0	39.0	33.0	39.0
14-15	37.730339936164484	38.0	37.0	41.0	33.0	41.0
16-17	37.54577293650994	38.0	36.5	41.0	32.5	41.0
18-19	37.458484614390265	38.0	36.0	41.0	32.0	41.0
20-21	37.47675445437811	38.0	36.0	41.0	33.0	41.0
22-23	37.32354879619429	38.0	36.0	40.5	32.5	41.0
24-25	37.2466358524956	38.0	36.5	40.5	32.0	41.0
26-27	36.925543620219145	38.0	36.0	40.0	32.0	41.0
28-29	36.87938268080822	38.0	36.0	40.0	32.0	41.0
30-31	36.63061312062225	38.0	35.5	40.0	31.0	41.0
32-33	36.561654955678684	37.5	36.0	40.0	30.5	41.0
34-35	36.31837569819457	37.5	35.0	40.0	30.5	41.0
36-37	36.08279936694696	37.0	35.0	40.0	30.0	41.0
38-39	36.02821734656571	37.0	35.0	40.0	30.0	41.0
40-41	36.21472323042009	37.5	35.0	40.0	30.0	41.0
42-43	36.244981085886124	37.5	35.0	40.0	30.0	41.0
44-45	36.05940770370718	37.0	35.0	40.0	30.0	41.0
46-47	35.93798910098207	37.0	35.5	40.0	29.5	41.0
48-49	35.70719231606289	37.5	34.5	40.0	29.0	41.0
50-51	35.53282560029988	37.5	34.5	40.0	28.0	41.0
52-53	35.54378914837268	37.5	35.0	40.0	27.5	41.0
54-55	35.348355704125595	37.5	34.0	40.0	27.0	41.0
56-57	35.10284152527064	37.0	34.5	40.0	26.5	41.0
58-59	34.91665366815846	37.0	34.0	39.5	27.0	41.0
60-61	34.72404516452412	36.5	34.0	39.0	26.0	41.0
62-63	34.48669990585586	36.5	34.0	39.0	26.0	41.0
64-65	34.303339969251596	37.0	33.0	38.5	25.0	40.5
66-67	33.807710345147896	35.5	33.5	38.0	25.0	40.0
68-69	33.67129300528181	36.5	33.0	37.0	24.0	40.0
70-71	33.250859391488966	35.0	32.5	37.0	24.0	39.5
72-73	33.014225197459155	35.0	32.5	37.0	23.5	39.0
74-75	32.81967760411497	35.0	32.0	37.0	23.0	39.0
76-77	31.23974314947791	34.0	29.5	36.0	21.5	37.0
78-79	32.35760046922046	35.0	31.5	36.5	23.0	37.0
80-81	32.462770936692024	35.0	32.0	36.0	23.0	37.5
82-83	32.360101423300684	35.0	32.0	37.0	23.5	37.0
84-85	32.02590751679582	35.0	32.0	36.0	22.5	36.5
86-87	31.903025477281588	35.0	31.5	36.0	23.0	36.5
88-89	31.849253577364138	35.0	31.5	36.0	22.0	37.0
90-91	31.607991606149106	34.5	31.0	35.0	21.5	36.5
92-93	31.3919641064435	34.5	31.0	35.0	21.0	36.5
94-95	31.51867263960963	34.0	31.0	35.0	21.0	36.5
96-97	31.24449875389005	34.0	31.0	35.0	20.0	37.0
98-99	31.09365604981398	34.0	31.0	35.0	20.0	36.0
100	29.85832345343955	34.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.07289453205758889
1101	2	-0.1641242913871963
1101	3	-0.08277088201531768
1101	4	-0.0739393069054799
1101	5	-0.13487462460582833
1101	6	-0.10701235065903347
1101	7	-0.08902180918547486
1101	8	-0.22751390405102256
1101	9	-0.17546016283169052
1101	10-11	-0.17010916632642648
1101	12-13	-0.10135587047383154
1101	14-15	-0.33470385265652425
1101	16-17	-0.2396837905397362
1101	18-19	-0.4335901173292811
1101	20-21	-0.3466810736805144
1101	22-23	-0.8589230045340415
1101	24-25	-0.3195820070293891
1101	26-27	-0.23945678581313956
1101	28-29	-0.3985401805824651
1101	30-31	-0.33800192182938105
1101	32-33	-0.33164843688491885
1101	34-35	-0.4055173037168771
1101	36-37	-0.47210278066439315
1101	38-39	-0.3874615139123563
1101	40-41	-0.437083572144644
1101	42-43	-0.45020938922136367
1101	44-45	-0.5373505522095243
1101	46-47	-0.404508537400865
1101	48-49	-0.5081148221516756
1101	50-51	-0.41445257013256054
1101	52-53	-0.5788676428705841
1101	54-55	-0.4840402416684455
1101	56-57	-0.6559118620343583
1101	58-59	-0.6773668671741717
1101	60-61	-0.7601539342338057
1101	62-63	-0.5276370186427499
1101	64-65	-0.49463324372486284
1101	66-67	-0.5170419282445025
1101	68-69	-0.51401443100157
1101	70-71	-0.7457596747974264
1101	72-73	-0.7311813324020378
1101	74-75	-0.6351724562904479
1101	76-77	-0.5647690991269698
1101	78-79	-0.5579951367433829
1101	80-81	-0.4854476298843835
1101	82-83	-0.4705156028727444
1101	84-85	-0.4741670940683669
1101	86-87	-0.512146392014948
1101	88-89	-0.5517297256794365
1101	90-91	-0.4800180503363194
1101	92-93	-0.49149756116242216
1101	94-95	-0.4935247474268749
1101	96-97	-0.664474565150126
1101	98-99	-0.7171642549955735
1101	100	-0.5214930795722097
1102	1	0.02918323987371707
1102	2	-0.10415262594425911
1102	3	-0.12158264294323118
1102	4	-0.0890926876695417
1102	5	-0.10612911808338055
1102	6	-0.12274110478160338
1102	7	-0.08907527893784817
1102	8	-0.181233186351065
1102	9	-0.12472327949797091
1102	10-11	-0.2073326064952994
1102	12-13	-0.2213580974978342
1102	14-15	-0.3149534849306477
1102	16-17	-0.3155647808119042
1102	18-19	-0.2980466332516869
1102	20-21	-0.3771730823127868
1102	22-23	-0.2836169685092855
1102	24-25	-0.2929366662058186
1102	26-27	-0.28301186965058633
1102	28-29	-0.3746003783473171
1102	30-31	-0.38265300323968177
1102	32-33	-0.2664066920610253
1102	34-35	-0.3915324488036518
1102	36-37	-0.45946779435542595
1102	38-39	-0.3952156868140335
1102	40-41	-0.37329045173415665
1102	42-43	-0.36780645678485513
1102	44-45	-0.2574220163864851
1102	46-47	-0.2623637447525411
1102	48-49	-0.32260129569400675
1102	50-51	-0.23083102145176326
1102	52-53	-0.3683131108039319
1102	54-55	-0.246313082903292
1102	56-57	-0.2798989311776765
1102	58-59	-0.2882808465629978
1102	60-61	-0.3695157908271369
1102	62-63	-0.48607961681213396
1102	64-65	-0.3776174479570784
1102	66-67	-0.4221713451184712
1102	68-69	-0.3107643876897441
1102	70-71	-0.43918374671230254
1102	72-73	-0.4873548939829746
1102	74-75	-0.5682495465706339
1102	76-77	-0.421844252496264
1102	78-79	-0.3943617461888884
1102	80-81	-0.4936992655977832
1102	82-83	-0.38768235363368575
1102	84-85	-0.40605730584344
1102	86-87	-0.45162183080331175
1102	88-89	-0.43166615402614994
1102	90-91	-0.5127002673775429
1102	92-93	-0.4899080051285729
1102	94-95	-0.591167507805487
1102	96-97	-0.5001626999076692
1102	98-99	-0.5273817952713777
1102	100	-0.6635576675919594
1103	1	-0.1455952674344161
1103	2	-0.15631666987466986
1103	3	-0.29711478861066354
1103	4	-0.12256639305427086
1103	5	-0.1837841182864537
1103	6	-0.2357427582999918
1103	7	-0.19011639188302354
1103	8	-0.1747353733410435
1103	9	-0.22602443721999776
1103	10-11	-0.3193781709870507
1103	12-13	-0.27549957642252565
1103	14-15	-0.2601327618998326
1103	16-17	-0.3532875821053878
1103	18-19	-0.3965050368779117
1103	20-21	-0.40478550353475384
1103	22-23	-0.39108145553007745
1103	24-25	-0.3249912509449189
1103	26-27	-0.37804047479944813
1103	28-29	-0.40006445885825315
1103	30-31	-0.5690633312888451
1103	32-33	-0.37317584973175144
1103	34-35	-0.5397456619289827
1103	36-37	-0.4623702202380855
1103	38-39	-0.42189223726734326
1103	40-41	-0.5045683157889158
1103	42-43	-0.5547570703050937
1103	44-45	-0.4531717274888081
1103	46-47	-0.552348745709395
1103	48-49	-0.533003993797081
1103	50-51	-0.5306698428394725
1103	52-53	-0.5036527670381403
1103	54-55	-1.2286765289005075
1103	56-57	-0.5362941002248789
1103	58-59	-0.5980635657475659
1103	60-61	-0.5300758584673773
1103	62-63	-0.5917621715742385
1103	64-65	-0.4369370349898887
1103	66-67	-0.4429388918520232
1103	68-69	-0.46671489357289886
1103	70-71	-0.5887614353351296
1103	72-73	-0.6770111795757643
1103	74-75	-0.7304330844762461
1103	76-77	-0.5871914674546836
1103	78-79	-0.4751950342080029
1103	80-81	-1.3645553548969858
1103	82-83	-0.4663783818875942
1103	84-85	-0.3454856612956938
1103	86-87	-0.4248063887367941
1103	88-89	-0.4052726591847531
1103	90-91	-0.3726250483088087
1103	92-93	-0.29639018864324385
1103	94-95	-0.32659641033476206
1103	96-97	-0.4853647223053841
1103	98-99	-0.3765879058232038
1103	100	-0.4431791916321828
1104	1	-0.038223626370779584
1104	2	-0.2269586444835312
1104	3	-0.13914346913835374
1104	4	-0.026800125038349165
1104	5	-0.07398386170161331
1104	6	-0.21915802745736102
1104	7	-0.2181118505568307
1104	8	-0.146636408137077
1104	9	-0.24402421368605332
1104	10-11	-0.19668331914537873
1104	12-13	-0.2369537080832842
1104	14-15	-0.4004105573622567
1104	16-17	-0.3714301364284083
1104	18-19	-0.3508908773671351
1104	20-21	-0.38298818292085457
1104	22-23	-0.34278949811069737
1104	24-25	-0.20194117728779304
1104	26-27	-0.29967831868540173
1104	28-29	-0.4952790343301885
1104	30-31	-0.4662140310920293
1104	32-33	-0.2506322643778134
1104	34-35	-0.37412937204570795
1104	36-37	-0.4413012560915561
1104	38-39	-0.20591995200791047
1104	40-41	-0.1642143599660315
1104	42-43	-0.2557280281891181
1104	44-45	-0.36086932284682405
1104	46-47	-0.29140453887814743
1104	48-49	-0.27825829012815717
1104	50-51	-0.21518766647685794
1104	52-53	-0.2958924105777143
1104	54-55	-0.284251138237515
1104	56-57	-0.3727456818735675
1104	58-59	-0.32579709996024064
1104	60-61	-0.34684593354293014
1104	62-63	-0.4068023914827137
1104	64-65	-0.6464485822888406
1104	66-67	-0.4446827350047613
1104	68-69	-0.3584425677095169
1104	70-71	-0.2227870804014742
1104	72-73	-0.3452219555639502
1104	74-75	-0.46618440428719055
1104	76-77	-0.3412790055924795
1104	78-79	-0.3149593040184939
1104	80-81	-0.21366980587356466
1104	82-83	-0.287961487796359
1104	84-85	-1.043489979635595
1104	86-87	-0.4556383051305346
1104	88-89	-0.37902435240417987
1104	90-91	-0.3197295831955067
1104	92-93	-0.21743099883102346
1104	94-95	-0.2626503224503267
1104	96-97	-0.19610111969670996
1104	98-99	-0.2845606286578608
1104	100	-1.444173319827211
1105	1	-0.015322762722284722
1105	2	-0.10771875532906705
1105	3	-0.0932522009572736
1105	4	-0.03894303281838063
1105	5	-0.05275760215985059
1105	6	-0.14160252798058792
1105	7	-0.08085444859859336
1105	8	-0.15178741171801136
1105	9	-0.19948306861377318
1105	10-11	-0.08165950065298944
1105	12-13	-0.10815895571695933
1105	14-15	-0.19957158881680215
1105	16-17	-0.16032795736215633
1105	18-19	-0.2020601949024723
1105	20-21	-0.235891578050186
1105	22-23	-0.1772669576149397
1105	24-25	-0.15153611831160418
1105	26-27	-0.25180374857863086
1105	28-29	-0.200097391983725
1105	30-31	-0.22333123080689887
1105	32-33	-0.2180448714168648
1105	34-35	-0.2434416962925141
1105	36-37	-0.29626814756711894
1105	38-39	-0.28907283687323115
1105	40-41	-0.21841097499437012
1105	42-43	-0.1553662980070598
1105	44-45	-0.3582772443097184
1105	46-47	-0.4415437041931085
1105	48-49	-0.3222732998431823
1105	50-51	-0.20858732178633232
1105	52-53	-0.3464917613688314
1105	54-55	-1.2318459581100072
1105	56-57	-0.2070799244590944
1105	58-59	-0.23760587607787187
1105	60-61	-0.10326419854303026
1105	62-63	-0.19185769168583988
1105	64-65	-0.16517331818070602
1105	66-67	-0.288526761750461
1105	68-69	-0.026739663216616805
1105	70-71	-0.057685703896652285
1105	72-73	-0.19719169630010924
1105	74-75	-0.15802700490363009
1105	76-77	-0.056620246739353064
1105	78-79	-0.11503318075874347
1105	80-81	0.034695510881611824
1105	82-83	0.05348285648221207
1105	84-85	-0.07291154464271798
1105	86-87	-0.11709303985497499
1105	88-89	-0.1813225331591397
1105	90-91	-0.16756768005096845
1105	92-93	-0.0638946365028481
1105	94-95	-0.15278266572865107
1105	96-97	-0.12488075900786555
1105	98-99	-0.04863591777219867
1105	100	-0.22488028809191007
1106	1	-0.04666345060494592
1106	2	-0.15252024781145224
1106	3	-0.1409441430576024
1106	4	-0.1361721299918628
1106	5	-0.09555515456300157
1106	6	-0.07931191928741299
1106	7	-0.02200241607934572
1106	8	-0.1332539291673811
1106	9	-0.0941730211133347
1106	10-11	-0.11621785221852576
1106	12-13	-0.133330878309188
1106	14-15	-0.156841878782636
1106	16-17	-0.1228006007660909
1106	18-19	-0.22479764568049632
1106	20-21	-0.1938340959610656
1106	22-23	-0.09336503173710042
1106	24-25	-0.08579016970702469
1106	26-27	0.02087039785080691
1106	28-29	-0.0037196771700038767
1106	30-31	-0.16990580038706327
1106	32-33	-0.05766470300533655
1106	34-35	-0.10530257310271196
1106	36-37	-0.20327231889889674
1106	38-39	-0.13824582306141053
1106	40-41	-0.05964524360472723
1106	42-43	-0.07243240026279096
1106	44-45	0.0037996961478015123
1106	46-47	-0.1680457516484566
1106	48-49	-0.12960267110481283
1106	50-51	-0.024859763644137445
1106	52-53	-0.07875558206539068
1106	54-55	-0.10330178489504505
1106	56-57	-0.021088184236432994
1106	58-59	0.0014599606104184204
1106	60-61	0.0069032092369383236
1106	62-63	-0.1809079043221402
1106	64-65	-0.052043639875378744
1106	66-67	0.06164398800179072
1106	68-69	0.0029094827403781665
1106	70-71	0.0650561647167649
1106	72-73	-0.06639450992248896
1106	74-75	-0.002841543676652236
1106	76-77	-0.07559634169114915
1106	78-79	0.08775339999581178
1106	80-81	0.1350761699795484
1106	82-83	0.08944656458612599
1106	84-85	0.1653126032289478
1106	86-87	0.2345155578328928
1106	88-89	0.020241289394068218
1106	90-91	0.09606310345918345
1106	92-93	0.20959181557914164
1106	94-95	0.1017953679980863
1106	96-97	0.13727786167264355
1106	98-99	0.06100601211070611
1106	100	0.25652812611903286
1107	1	-0.04231020557978127
1107	2	-0.09883578256110326
1107	3	-0.140355906027267
1107	4	-0.07235606135994033
1107	5	-0.13143491126484008
1107	6	-0.1055020194535814
1107	7	-0.1526755932897217
1107	8	-0.22759160108485332
1107	9	-0.2755850302695464
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2316	82-83	-0.2031859726857661
2316	84-85	-0.7398727006728159
2316	86-87	-0.11318646570090607
2316	88-89	-0.029629526218766244
2316	90-91	0.08186828182039108
2316	92-93	0.1873688909238247
2316	94-95	0.19994275332165046
2316	96-97	0.2086046838310196
2316	98-99	0.22992413618633734
2316	100	0.13040905393945934
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	39.0
13	212.0
14	770.0
15	2052.0
16	4466.0
17	8234.0
18	13466.0
19	19143.0
20	25302.0
21	32224.0
22	39964.0
23	45890.0
24	49929.0
25	55333.0
26	62667.0
27	73536.0
28	87866.0
29	107166.0
30	131567.0
31	160958.0
32	200997.0
33	259954.0
34	368517.0
35	652888.0
36	959198.0
37	708830.0
38	665609.0
39	129077.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.9936480373268	18.33822623267037	13.159901485469025	23.508224244533807
2	16.5767609611244	20.82410160749182	38.70971918836996	23.88941824301383
3	19.520615017479653	25.836554188982486	29.993302428737863	24.6495283648
4	13.250899005206401	16.50332700772349	36.75267687560086	33.49309711146925
5	13.55421525804586	36.7605454921353	33.83678064447449	15.848458605344357
6	30.667251959178316	35.35403106859246	18.634085931545027	15.3446310406842
7	27.365513193539915	32.32597100417555	22.034418001694224	18.274097800590308
8	26.540791707254918	33.84840339173931	20.56965355100967	19.041151349996095
9	27.056358859937323	15.949015432363264	20.600200207852826	36.39442549984659
10-11	25.49700813295844	25.43929062854537	28.148005889507715	20.915695348988475
12-13	25.915285044717564	23.53184525176536	28.005543442419377	22.5473262610977
14-15	23.287101789896543	24.83546455404682	26.58640204794502	25.29103160811162
16-17	22.497065525985263	27.30045756523996	27.272281682377653	22.930195226397124
18-19	22.259101016453144	26.616059795229695	29.063500577483488	22.061338610833673
20-21	23.20053350788111	25.724851941801198	28.95334529067805	22.121269259639647
22-23	22.868030859203728	25.59970069297468	28.85628813459527	22.675980313226322
24-25	22.718914608556613	25.656183784072624	28.85875853678086	22.7661430705899
26-27	22.562122135816725	25.463940241397324	29.1842388370899	22.78969878569605
28-29	22.38622046192139	26.179858303547753	29.032131427878475	22.401789806652374
30-31	23.210189252672837	25.63536646285325	28.923089697531353	22.23135458694256
32-33	22.898112093837515	25.840317395463092	28.864609148056086	22.39696136264331
34-35	23.12202067797338	25.954526298395308	28.73099469110724	22.19245833252407
36-37	23.04129858925037	25.81123293183668	28.755427521281064	22.392040957631888
38-39	22.9432736055467	26.21578579382137	28.479572077598853	22.361368523033075
40-41	22.922657833077682	26.061053661566273	28.58678983708412	22.429498668271926
42-43	22.757311859139108	26.178435937186258	28.174336564916764	22.889915638757873
44-45	22.711921012987066	26.17148723387201	28.248462166100445	22.86812958704047
46-47	22.827552101382338	26.121674512139826	28.045829731239618	23.004943655238215
48-49	22.837436564617477	26.22272955569051	28.04344669166861	22.896387188023404
50-51	22.570838011195626	26.56086784400119	28.044430202457743	22.82386394234544
52-53	22.74971089171344	26.77656052961	27.414864764736812	23.05886381393974
54-55	22.596836944290107	26.41434206860806	27.783827409004886	23.20499357809695
56-57	22.90705015697432	26.423466078502777	27.431488662417657	23.23799510210524
58-59	22.9376024308403	26.415359395315324	27.661310974995672	22.985727198848704
60-61	23.07800597840663	26.540066524930406	27.84518413554069	22.536743361122273
62-63	22.80530027195162	26.605255429630336	27.798067341164778	22.791376957253267
64-65	22.690461616365777	26.527925930774447	27.596816192244265	23.18479626061551
66-67	22.688454864473865	26.38178518226768	27.382626443808565	23.547133509449882
68-69	22.44520407818302	26.44359781144101	27.555253181732663	23.555944928643306
70-71	22.666291267762517	26.27605999669128	27.564527680702682	23.49312105484352
72-73	22.567947244819297	26.51764962656683	27.334247606301705	23.58015552231217
74-75	22.46501152050292	26.86666366605401	27.080837532649642	23.58748728079343
76-77	22.35959243958223	26.385943516465876	27.401741194222762	23.852722849729126
78-79	22.345041692797345	26.493276447492313	27.424537357254046	23.737144502456292
80-81	22.491548911094483	26.563166302263014	27.10648361745446	23.838801169188038
82-83	22.523568874278823	26.558096104963962	27.200454345687646	23.71788067506957
84-85	22.38682924598728	26.82028573146052	26.965797408821434	23.82708761373077
86-87	22.22340733360625	26.77099825562565	27.05199296002736	23.95360145074074
88-89	22.01205811917262	26.60541154183586	27.055068370238555	24.32746196875296
90-91	22.478223564892595	26.37933178172701	27.06679613845261	24.07564851492779
92-93	22.394184537201976	26.56778034346611	27.02596670231594	24.012068417015968
94-95	22.11298676730906	26.41526824767622	27.13392670257133	24.337818282443386
96-97	21.865135012854378	26.38114829439411	27.33726049798273	24.416456194768788
98-99	22.002477230401336	26.388093675232504	26.990241101473554	24.619187992892606
100	21.83669417900516	26.318173392262707	27.092124889384124	24.753007539348015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2307.0
1	1733.5
2	3130.0
3	4318.0
4	2599.5
5	1871.5
6	2644.0
7	4770.0
8	7139.0
9	8062.0
10	6938.5
11	5454.0
12	5395.5
13	5864.5
14	6407.0
15	6918.5
16	7420.0
17	7977.5
18	8591.0
19	9240.5
20	9896.0
21	10959.5
22	12509.0
23	14481.5
24	17258.5
25	20407.5
26	24798.5
27	30164.5
28	34803.0
29	40394.0
30	47297.0
31	54029.5
32	61218.0
33	69128.5
34	77546.5
35	85709.0
36	96573.0
37	107288.0
38	113912.5
39	120027.0
40	124410.0
41	127577.0
42	132608.5
43	140041.0
44	146729.5
45	153874.5
46	162472.5
47	176710.0
48	200565.5
49	210575.5
50	210068.0
51	204846.5
52	192368.0
53	185006.0
54	178362.5
55	167821.5
56	153289.0
57	139755.0
58	128095.0
59	113488.0
60	95230.5
61	78137.5
62	63896.5
63	50434.5
64	38210.0
65	29210.0
66	22331.0
67	17492.5
68	14052.5
69	11015.0
70	8794.0
71	7110.5
72	5817.5
73	4900.5
74	4009.0
75	3122.5
76	2319.5
77	1592.0
78	1088.0
79	743.0
80	537.5
81	401.0
82	270.5
83	167.5
84	120.5
85	86.0
86	53.0
87	41.0
88	26.5
89	12.0
90	10.0
91	7.5
92	4.0
93	2.5
94	1.5
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042992694324614986
2	0.0097617255278165
3	0.0
4	1.8495901000073367E-4
5	5.137750277798157E-4
6	0.0
7	2.055100111119263E-5
8	6.16530033335779E-5
9	0.0
10-11	3.082650166678895E-5
12-13	2.774385150011005E-4
14-15	0.01187847864226934
16-17	0.001633804588339814
18-19	6.16530033335779E-5
20-21	7.295605394473383E-4
22-23	7.50111540558531E-4
24-25	0.004788383258907882
26-27	0.015803719854507133
28-29	0.01259776368116108
30-31	0.009525389015037784
32-33	0.007007891378916687
34-35	0.00878555297503485
36-37	0.00476783225779669
38-39	0.004130751223349718
40-41	0.005600147802799992
42-43	0.006576320355581642
44-45	0.006843483370027146
46-47	0.014735067796725116
48-49	0.017560830449514103
50-51	0.01703677992117869
52-53	0.011333877112822736
54-55	0.0035655986927919216
56-57	0.0036786291989034806
58-59	0.009556215516704573
60-61	0.002013998108896878
62-63	8.220400444477052E-5
64-65	1.7468350944513736E-4
66-67	7.50111540558531E-4
68-69	3.082650166678895E-5
70-71	1.6440800888954104E-4
72-73	0.003000446162234124
74-75	0.0055487703000220105
76-77	0.005332984788354487
78-79	0.0035861496939031136
80-81	4.110200222238526E-5
82-83	9.145195494480721E-4
84-85	2.157855116675226E-4
86-87	3.0826501666788946E-4
88-89	2.0551001111192632E-4
90-91	3.6991802000146734E-4
92-93	3.9046902111265997E-4
94-95	3.082650166678895E-5
96-97	0.0018804166016741257
98-99	5.137750277798157E-4
100	1.0275500555596316E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4865943.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.53219623277366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.64868991001632	42.37170880189756
2	15.944198152974145	18.346094738222718
3	5.024441796332713	8.672015141602902
4	1.9795899266206447	4.555606244750438
5	0.950184665467272	2.733310531551774
6	0.5922714813447759	2.0444807452681912
7	0.366455807794019	1.475810521125156
8	0.24940479052509235	1.1479044279906736
9	0.18996422212851724	0.9836153014233653
>10	0.9740183861678898	9.801655734939567
>50	0.05321562623385948	2.094956047656657
>100	0.025245110247996482	2.583910398631409
>500	0.0012257260124267815	0.46809311224735706
>1k	8.755185075445194E-4	1.0211360135829661
>5k	4.377592537722474E-5	0.18083010426749524
>10k+	1.7510370150889895E-4	1.5188721348417968
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19129	0.39312010025600386	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17990	0.36971250999035543	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	11855	0.24363211817318864	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11334	0.23292504659425728	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7180	0.1475561879783631	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.110200222238526E-5
2	0.0	0.0	0.0	0.0	4.110200222238526E-5
3	0.0	0.0	0.0	0.0	4.110200222238526E-5
4	0.0	0.0	0.0	0.0	4.110200222238526E-5
5	0.0	0.0	0.0	0.0	4.110200222238526E-5
6	0.0	0.0	0.0	0.0	6.16530033335779E-5
7	0.0	0.0	0.0	0.0	6.16530033335779E-5
8	0.0	0.0	0.0	0.0	8.220400444477052E-5
9	0.0	0.0	0.0	0.0	8.220400444477052E-5
10-11	0.0	0.0	0.0	0.0	8.220400444477052E-5
12-13	0.0	0.0	0.0	0.0	8.220400444477052E-5
14-15	0.0	0.0	0.0	0.0	1.4385700777834842E-4
16-17	0.0	0.0	0.0	0.0	2.055100111119263E-4
18-19	0.0	0.0	0.0	0.0	2.055100111119263E-4
20-21	0.0	0.0	0.0	9.247950500036683E-5	2.055100111119263E-4
22-23	0.0	0.0	0.0	3.185405172234858E-4	2.3633651277871524E-4
24-25	0.0	0.0	0.0	6.473565350025678E-4	2.8771401555669683E-4
26-27	0.0	0.0	0.0	0.0014282945772278878	2.8771401555669683E-4
28-29	0.0	0.0	0.0	0.004171853225572104	2.8771401555669683E-4
30-31	0.0	0.0	0.0	0.01246418217393833	2.979895161122931E-4
32-33	0.0	0.0	0.0	0.03351868281235518	3.0826501666788946E-4
34-35	0.0	0.0	0.0	0.06627697858359624	3.0826501666788946E-4
36-37	2.055100111119263E-5	0.0	0.0	0.11890809242936057	3.0826501666788946E-4
38-39	4.110200222238526E-5	0.0	0.0	0.19725878416578246	3.0826501666788946E-4
40-41	4.110200222238526E-5	0.0	0.0	0.2886388106067005	3.0826501666788946E-4
42-43	4.110200222238526E-5	0.0	0.0	0.375374310796489	3.185405172234858E-4
44-45	4.110200222238526E-5	0.0	0.0	0.4849933507235905	3.288160177790821E-4
46-47	6.16530033335779E-5	0.0	0.0	0.6135912401768784	3.493670188902747E-4
48-49	8.220400444477052E-5	0.0	0.0	0.7464637378612943	3.9046902111265997E-4
50-51	8.220400444477052E-5	0.0	0.0	0.8882450945274123	4.110200222238526E-4
52-53	8.220400444477052E-5	0.0	0.0	1.03665414905189	4.212955227794489E-4
54-55	8.220400444477052E-5	0.0	0.0	1.2043092161169993	4.4184652389064156E-4
56-57	8.220400444477052E-5	0.0	0.0	1.4058015065116876	4.5212202444623785E-4
58-59	8.220400444477052E-5	0.0	0.0	1.6268583499642308	4.6239752500183413E-4
60-61	8.220400444477052E-5	0.0	2.055100111119263E-5	1.8509053640784532	4.7267302555743047E-4
62-63	8.220400444477052E-5	0.0	2.055100111119263E-5	2.084765481223269	4.7267302555743047E-4
64-65	8.220400444477052E-5	0.0	2.055100111119263E-5	2.320238851955315	4.7267302555743047E-4
66-67	8.220400444477052E-5	0.0	2.055100111119263E-5	2.5906386490758315	4.7267302555743047E-4
68-69	8.220400444477052E-5	0.0	2.055100111119263E-5	2.904729874558744	4.7267302555743047E-4
70-71	8.220400444477052E-5	0.0	2.055100111119263E-5	3.240440753210631	4.7267302555743047E-4
72-73	8.220400444477052E-5	0.0	2.055100111119263E-5	3.553792142653541	5.343260288910084E-4
74-75	8.220400444477052E-5	0.0	2.055100111119263E-5	3.873791780955922	5.343260288910084E-4
76-77	8.220400444477052E-5	0.0	2.055100111119263E-5	4.226190072510097	5.343260288910084E-4
78-79	8.220400444477052E-5	0.0	2.055100111119263E-5	4.637631390256729	5.343260288910084E-4
80-81	8.220400444477052E-5	0.0	2.055100111119263E-5	5.058330934003953	5.651525305577973E-4
82-83	8.220400444477052E-5	0.0	2.055100111119263E-5	5.484425115542866	6.987340377805494E-4
84-85	8.220400444477052E-5	0.0	2.055100111119263E-5	5.919345952059036	7.192850388917421E-4
86-87	8.220400444477052E-5	0.0	2.055100111119263E-5	6.36846753034304	7.295605394473383E-4
88	8.220400444477052E-5	0.0	2.055100111119263E-5	6.733247800066708	7.398360400029347E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	6550	0.0	47.285732	2
GTACCTG	7935	0.0	39.52445	1
ACCTGGG	7845	0.0	38.817135	3
CCTGGGG	7185	0.0	29.040066	4
CATGGGG	12915	0.0	22.996624	4
ATGGGGG	7795	0.0	21.823975	5
TATAACG	475	0.0	21.767826	2
GTACATG	24665	0.0	21.446592	1
TACATGG	25295	0.0	20.568329	2
TGGGGGG	7540	0.0	20.25578	6
ACATGGG	24905	0.0	20.11463	3
GAGTACT	18815	0.0	20.023449	12-13
CTGGGGG	4600	0.0	19.614841	5
GTATCAA	36415	0.0	18.580929	1
GGTATCA	29375	0.0	18.504053	1
TAACGCA	510	0.0	18.428978	4
TATCACG	410	1.8189894E-12	18.340965	2
AGTACTT	19680	0.0	18.25963	12-13
ATAACGC	545	0.0	17.245464	3
GTACTTT	21780	0.0	17.14483	14-15
>>END_MODULE