FastQCFastQC Report
Mon 6 Jun 2016
ERR522958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5299647
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170760.32221013965647144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134820.2543943021110651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81020.15287810678711242No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85500.048.2082941
GTATCAA152050.041.8073541
TACCTGG57800.037.7344672
TCAACGC180500.034.4734654
ATCAACG183200.033.8659823
CAACGCA184400.033.7950135
TATCAAC189500.032.8417932
AACGCAG195000.032.1916546
GTACCTG72850.030.4857041
ACCTGGG72350.029.3639953
ACGCAGA220600.028.3491387
GTACATG169900.027.5281351
CGCAGAG229100.027.2140188
TATCACG6600.026.3515322
TACATGG174450.026.0018582
ACATGGG177500.024.7057743
GCAGAGT253300.024.1851149
CATGGGG132400.022.9662674
ATGGGGG80950.021.5971345
AGAGTAC225750.020.62230910-11
Produced by FastQC (version 0.11.5)