FastQCFastQC Report
Mon 6 Jun 2016
ERR522958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5299647
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155750.29388749854471435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129090.24358226123362559No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77080.14544364936004228No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81850.048.8528941
TACCTGG58100.040.2293662
GTATCAA141650.038.557221
GTACCTG69350.033.91671
ACCTGGG68050.033.2172933
TCAACGC176750.030.4960144
ATCAACG178800.030.1469383
CAACGCA181500.029.827375
TATCAAC185300.029.1613432
AACGCAG189600.028.7753546
GTACATG164500.026.767021
TACATGG168150.025.4231822
ACGCAGA216500.025.1349167
ACATGGG171850.024.2287753
TATAACG4500.024.0368882
CGCAGAG226400.024.0216358
TATCACG6400.022.7795242
CCTGGGG69850.022.6053874
CATGGGG122750.022.5875744
GCAGAGT253550.021.4123339
Produced by FastQC (version 0.11.5)