FastQCFastQC Report
Mon 6 Jun 2016
ERR522957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5812761
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT412220.7091638551800084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202150.3477693302717934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157380.27074913281313306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93770.16131748750722763No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC88780.15273292674513886No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC82570.14204953549612653No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA64860.11158208637857293No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG64040.11017139703490303No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG51150.032.1711922
GTACATG209150.029.1374721
TACATGG211800.028.1473852
GTACCTG61650.027.3057881
ACATGGG213150.026.9786153
ACCTGGG62850.025.885073
CATGGGG146000.024.5064454
ATGGGGG89300.023.218585
GAGTACT174350.020.1036412-13
AGAGTAC261500.019.90637410-11
TGGGGGG88500.018.2634326
AGTACTT183000.018.24193412-13
GAGTACA156900.018.0117661
TATAACG5250.017.9108182
GTACTTT193900.017.8947214-15
TATCACG6400.016.8963382
AGTACAT155700.016.7892362
CATGGGA75250.016.7446784
GAGTACG7100.015.8948951
CCTGGGG68750.015.3871544
Produced by FastQC (version 0.11.5)