FastQCFastQC Report
Mon 6 Jun 2016
ERR522957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5812761
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT421150.7245266062031451No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187620.32277260324310597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154450.26570849893880033No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT116280.20004263034382455No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC93240.1604057004924166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89240.1535242890598805No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC85250.14666008115592571No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG67280.11574534029525727No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA66010.11356049216542706No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA61450.10571568313233592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG54700.031.4499742
GTACATG213150.026.986821
GTACCTG65750.026.8253961
TACATGG219200.025.81742
ACCTGGG69350.024.5305043
ACATGGG222350.024.4112053
CATGGGG149550.024.1962814
TATAACG5000.022.5614912
ATGGGGG89650.022.3829965
TATCACG5950.020.5391732
GAGTACT175800.019.57664912-13
AGAGTAC269350.018.60710710-11
TATACCG3851.4551915E-1118.3092775
TGGGGGG84200.018.3063766
GTACTTT191900.017.63694414-15
CCTGGGG65150.017.6002884
GAGTACA167200.017.5251751
AGTACTT184100.017.22559712-13
ATAACGC6700.016.8337483
AGTACAT163000.016.4655062
Produced by FastQC (version 0.11.5)