FastQCFastQC Report
Mon 6 Jun 2016
ERR522956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43731383
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT150550.012.2630281
TCGAATG104350.012.1550352
CGCGCTA25000.011.8391049
CGGAAAT114450.011.2465861
CGGCGAT55600.010.6458831
CGAATGT103800.010.6356233
CGGCGGT104000.010.389161
CCGGAAT119850.010.3870871
CGACGAT81050.010.1431061
CCGGGAT122400.010.0939291
CGGGAAT124450.09.3991891
CGACAAT52750.09.3508851
CGAAGAT169800.09.3234821
CCGAAAT73700.09.2424351
TGCGCGG74300.09.1669587
TTGCGCG31850.09.143366
CTGGAAT428850.09.0919821
CGAGAAT115650.08.8145471
GTTCGAT69950.08.7977081
CGGGGAT133900.08.7007561
Produced by FastQC (version 0.11.5)