FastQCFastQC Report
Mon 6 Jun 2016
ERR522956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43731383
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT80900.013.0812591
CTCGAAT152150.012.6125741
TCGAATG109350.011.516782
CGAATGT108700.011.2815283
CGCGCTA21450.011.1767229
CGACAAT55850.010.6111611
CCGGGAT121300.010.5856461
CCGGAAT116800.010.1478321
CGGAAAT110100.09.9109711
GTTCGAT72750.09.7624651
TTGCGCG30500.09.70966
CGAAGAT175350.09.3881021
CGGGAAT126800.08.8653151
CGCGGAT46750.08.8535331
TTCGATT71700.08.8476862
CGGGGAT135850.08.7940641
CCGGGTT106950.08.7516081
CGGGTAT38750.08.7392931
CTCGGAT175650.08.6223021
CTGGAAT439650.08.59061051
Produced by FastQC (version 0.11.5)