FastQCFastQC Report
Mon 6 Jun 2016
ERR522955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6623054
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT143540.21672781167117167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG79150.036.7178342
ACCTGGG100850.028.5842843
TGGGGGG99800.027.1209966
GTACCTG109200.027.0931591
CATGGGG235700.025.3559194
ATGGGGG135800.024.789095
GTACATG383000.024.3163321
TACATGG385650.023.8392682
ACATGGG400200.022.4674383
TATATAA12500.019.5628812
CCTGGGG109500.019.1090584
TATAAGT11900.018.966524
GTATAGT9600.018.1285111
GTAAGTA27350.017.3661482
ATATAAG11950.017.31523
GTATATA11150.016.8739511
GTATACG3401.9794243E-816.600991
GTATATG17600.016.5695481
GTATTAC11700.016.080731
GTATTAA15750.015.8280331
Produced by FastQC (version 0.11.5)