FastQCFastQC Report
Mon 6 Jun 2016
ERR522955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6623054
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT142880.215731292542685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG75050.036.0725252
TGGGGGG92200.026.7072856
GTACCTG102550.026.5485731
ACCTGGG100450.026.431873
ATGGGGG133250.024.9334035
CATGGGG239650.024.8247934
GTACATG383250.024.0721651
TACATGG388750.023.5275762
ACATGGG394650.022.743153
CCTGGGG110350.017.161774
ATATGTA18150.016.8397251
TATGTAA18950.016.6176782
GTATATA11500.016.3553731
GTATATG17700.015.9395581
GTAAGTA28800.015.8301132
TATACCG6850.015.7785325
TATATAA12100.015.5374372
GTATAGC16650.015.5326931
GTATAGG14550.015.5123121
AGTACAT294550.015.494052
Produced by FastQC (version 0.11.5)