FastQCFastQC Report
Mon 6 Jun 2016
ERR522954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5810198
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371370.6391692675533605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307910.5299475164185454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158720.27317485565896377No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64020.11018557371022468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233250.041.29121
GTATCAA385100.035.3701021
TCAACGC461150.029.338614
ATCAACG462000.029.2665623
CAACGCA466600.028.9647145
AACGCAG475550.028.4235866
TATCAAC476700.028.3874632
ACGCAGA528700.025.4597557
CGCAGAG533450.025.2150068
GTACATG369100.024.5384961
GAGTACT267150.024.26050612-13
GCAGAGT565050.023.4627599
ACATGGG371500.023.3279153
GTACTTT280650.022.93447714-15
TACATGG396650.022.538922
CATGGGG133600.021.5974274
CATGGGA185700.020.9282884
AGAGTAC479700.020.2666610-11
CAGAGTA558150.020.13245610-11
ATGGGAT88500.019.5933275
Produced by FastQC (version 0.11.5)