FastQCFastQC Report
Mon 6 Jun 2016
ERR522954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5810198
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327280.5632854508572686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283490.4879179676837175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147040.2530722705147054No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT86070.14813608761697966No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59460.10233730416760324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA237850.041.360781
GTATCAA385550.034.289681
TCAACGC462500.028.2666684
ATCAACG463850.028.1745113
CAACGCA467200.027.9823065
AACGCAG477450.027.4005556
TATCAAC480150.027.270882
GTACATG367000.025.5749421
ACGCAGA531950.024.5400667
CGCAGAG537000.024.2697128
ACATGGG373150.023.9907883
CATGGGG134300.023.5137834
GAGTACT272300.023.43951612-13
TACATGG396300.023.28532
GCAGAGT569200.022.6325329
GTACTTT281400.022.38749914-15
GGATATG22200.021.1926291
CATGGGA186150.020.7509524
AGAGTAC481950.020.42055110-11
CAGAGTA558900.019.75339110-11
Produced by FastQC (version 0.11.5)