##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522953_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1505454
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.406359809067563	33.0	31.0	34.0	28.0	34.0
2	31.669313044437093	33.0	31.0	34.0	28.0	35.0
3	31.63334250000332	34.0	31.0	34.0	27.0	36.0
4	33.881116925525454	35.0	34.0	37.0	32.0	37.0
5	33.967233140301865	35.0	34.0	37.0	31.0	37.0
6	34.348425790492435	35.0	35.0	37.0	31.0	37.0
7	34.24596367607379	35.0	34.0	37.0	31.0	37.0
8	34.453243340547104	36.0	34.0	37.0	31.0	37.0
9	35.50498653562314	37.0	35.0	39.0	31.0	39.0
10-11	35.516178508277235	37.0	34.5	39.0	31.0	39.0
12-13	35.602193756833486	37.5	35.0	39.0	31.0	39.0
14-15	36.18381664268719	38.0	35.5	40.0	31.0	41.0
16-17	36.30721131299927	38.0	36.0	40.0	30.5	41.0
18-19	36.29699645422576	38.0	35.5	40.0	30.5	41.0
20-21	36.54677359786483	38.5	36.0	40.0	31.0	41.0
22-23	36.42207267707947	38.0	35.5	40.0	31.0	41.0
24-25	36.428855016493365	38.0	36.0	40.0	31.0	41.0
26-27	36.19503883878219	37.5	36.0	40.0	30.0	41.0
28-29	35.97217782808376	38.0	35.0	40.0	30.0	41.0
30-31	35.69816646672698	37.0	35.5	40.0	29.5	41.0
32-33	35.416618176310934	37.5	34.5	40.0	28.0	41.0
34-35	35.2793160734237	37.5	34.5	40.0	27.5	41.0
36-37	35.12160916241878	37.5	35.0	40.0	27.5	41.0
38-39	34.875126041712335	36.5	35.0	40.0	25.5	41.0
40-41	34.711807534471326	37.0	34.0	40.0	24.5	41.0
42-43	34.31845310451199	36.0	35.0	40.0	23.5	41.0
44-45	33.997133090748704	37.0	33.0	40.0	20.5	41.0
46-47	33.39119660912921	36.5	33.0	39.0	18.5	41.0
48-49	33.03112383374052	37.0	33.0	39.0	13.5	41.0
50-51	32.00242717479246	36.0	31.5	38.5	11.0	40.0
52-53	32.169456854875676	36.5	31.5	38.5	10.0	39.5
54-55	32.938210001766905	37.5	33.0	39.5	10.0	40.5
56-57	32.94589240189338	37.0	32.5	40.0	10.0	41.0
58-59	32.876553850200665	37.0	33.0	40.0	10.0	41.0
60-61	32.48973532236787	36.5	32.0	39.5	9.0	41.0
62-63	32.204540955751554	36.5	31.0	39.0	9.0	41.0
64-65	31.82537825798729	36.0	31.0	39.0	9.0	41.0
66-67	31.47766520929899	36.0	30.5	38.0	9.0	40.0
68-69	31.04347857855504	35.0	29.5	37.5	8.0	40.0
70-71	30.587134844372528	35.0	29.0	37.0	8.0	39.0
72-73	30.070260200577366	35.0	28.0	37.0	8.0	39.0
74-75	29.579957939598287	34.5	26.5	36.0	7.0	38.5
76-77	29.19813458265746	34.0	26.0	36.5	7.0	38.0
78-79	28.791437001728383	34.0	26.0	36.0	7.0	37.0
80-81	28.425061144345825	34.0	24.5	35.5	7.0	37.5
82-83	28.006230678585993	34.0	24.0	35.0	7.0	37.0
84-85	27.673675847950186	34.0	22.0	35.0	7.0	36.5
86-87	27.31522019271263	33.0	20.0	35.0	7.0	37.0
88-89	27.00012720415237	33.0	19.0	35.0	7.0	36.5
90-91	26.632902101293034	33.0	17.5	35.0	7.0	36.0
92-93	26.157378438663685	32.5	17.0	35.0	7.0	35.0
94-95	25.956645304340086	32.0	16.0	35.0	7.0	36.0
96-97	25.69758358608101	32.0	16.5	35.0	7.0	35.0
98-99	25.485396099781198	32.0	15.0	35.0	7.0	36.0
100	24.154780551248994	29.0	10.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.006217589493765274
1101	2	0.03112536530744947
1101	3	0.08587226199354348
1101	4	0.1997748479167285
1101	5	0.23502535859028484
1101	6	0.24033420493660884
1101	7	0.296451127631542
1101	8	0.2360420789617379
1101	9	0.33378276018456887
1101	10-11	0.2740108980660878
1101	12-13	0.24165544651367554
1101	14-15	0.21716593062023293
1101	16-17	0.21824647881654613
1101	18-19	0.13181105041510932
1101	20-21	0.1989435864133
1101	22-23	0.18550804808656807
1101	24-25	0.23811368709387182
1101	26-27	0.13917326421480425
1101	28-29	0.2603619578713108
1101	30-31	0.2035073432340866
1101	32-33	0.21914826229453155
1101	34-35	0.22317967994312227
1101	36-37	0.2121793884472183
1101	38-39	0.22142939889089774
1101	40-41	0.1944929318295081
1101	42-43	0.1582859332229063
1101	44-45	0.10061873142922195
1101	46-47	0.39829904849039366
1101	48-49	0.26117796231879
1101	50-51	-0.09727340096032577
1101	52-53	0.03579539008408261
1101	54-55	0.17720353308323666
1101	56-57	0.17198994393595513
1101	58-59	0.010006571596285596
1101	60-61	-0.104968871445827
1101	62-63	-0.12531659375363802
1101	64-65	-0.01323026439501973
1101	66-67	0.06099685898047369
1101	68-69	0.002847601767825836
1101	70-71	-0.022450865609421555
1101	72-73	0.025502437732075833
1101	74-75	-0.0047292179050018035
1101	76-77	-0.22416587862309
1101	78-79	-0.19708184992991917
1101	80-81	-0.21727844095617854
1101	82-83	-0.4660878488952811
1101	84-85	-0.39881108726692105
1101	86-87	-0.5160517925471915
1101	88-89	-0.39748848663975167
1101	90-91	-0.5803428980098069
1101	92-93	-0.48144747351835804
1101	94-95	-0.737304074004161
1101	96-97	-0.7352676123825326
1101	98-99	-0.6428497624919878
1101	100	-0.5022073920736148
1102	1	-0.25594102761553117
1102	2	-0.26004455112709124
1102	3	-0.29272304242348923
1102	4	-0.3374595333009438
1102	5	-0.15174543329192858
1102	6	-0.2850438292854349
1102	7	-0.12053852616870131
1102	8	-0.09476771013495977
1102	9	-0.30685392903150444
1102	10-11	-0.24216697698365408
1102	12-13	-0.2304754727063809
1102	14-15	-0.4156096364311068
1102	16-17	-0.2596007157715903
1102	18-19	-0.12096650629002426
1102	20-21	-0.2002486141438098
1102	22-23	-0.13970683452386368
1102	24-25	-0.2329696794003553
1102	26-27	-0.24685638162682721
1102	28-29	-0.21551945876219492
1102	30-31	-0.18929508414355212
1102	32-33	-0.16523295537360383
1102	34-35	-0.23514304190327806
1102	36-37	-0.6340939899849403
1102	38-39	-0.282556872497878
1102	40-41	-0.6993799292926539
1102	42-43	-0.7741961598928881
1102	44-45	-0.1391902618059575
1102	46-47	0.059599287248850885
1102	48-49	-0.45185128559207755
1102	50-51	-0.3765292704390326
1102	52-53	-0.1550143990126145
1102	54-55	-0.0681506252932067
1102	56-57	0.0758916550382196
1102	58-59	0.0057487124637773945
1102	60-61	-0.17589207080913383
1102	62-63	-0.2666954238372057
1102	64-65	-0.05433750673485349
1102	66-67	0.08141369145162614
1102	68-69	0.10554457749404733
1102	70-71	-0.06953005383067179
1102	72-73	-0.0035007457140032727
1102	74-75	-0.06913829072633249
1102	76-77	-0.00570388101067465
1102	78-79	-0.1866033381909631
1102	80-81	-0.26738488743449196
1102	82-83	-0.34274821499158037
1102	84-85	-0.4137374700763097
1102	86-87	-0.3839815577680419
1102	88-89	-0.6082455499107411
1102	90-91	-0.28295431862262177
1102	92-93	-0.3795891368689368
1102	94-95	-0.7856078941394564
1102	96-97	-0.5562485117060518
1102	98-99	-0.3533650828262509
1102	100	-0.6534549050063632
1103	1	-0.02076281229380328
1103	2	-0.03573402084530031
1103	3	-0.08451521633826076
1103	4	0.11121769486278765
1103	5	0.11284273393442845
1103	6	-0.012747777320960552
1103	7	-0.01512784657056443
1103	8	0.0021606677248655615
1103	9	0.1005177453992161
1103	10-11	0.007946352333448203
1103	12-13	0.08761904394408049
1103	14-15	0.10127320093734937
1103	16-17	0.008065485648401705
1103	18-19	0.0894209209157637
1103	20-21	-0.027476827576712992
1103	22-23	-0.15252447969971428
1103	24-25	-0.06855170497301799
1103	26-27	-0.23862907085198515
1103	28-29	-0.5277827346952293
1103	30-31	-0.4091812975327187
1103	32-33	-0.3901282748728008
1103	34-35	-0.42526887720993045
1103	36-37	-0.4960993920157293
1103	38-39	-0.5808987850593326
1103	40-41	-0.6233970957018542
1103	42-43	-0.4658623792069889
1103	44-45	-0.5453953401523037
1103	46-47	-0.4541351821611883
1103	48-49	-0.4481350284021275
1103	50-51	-0.48760989530110166
1103	52-53	-0.41327874063887293
1103	54-55	-0.1526170032683467
1103	56-57	-0.07718717444886636
1103	58-59	-0.12158820764303613
1103	60-61	-0.16947330705304609
1103	62-63	-0.2010268007486431
1103	64-65	-0.38460249851350525
1103	66-67	-0.44080109146308644
1103	68-69	-0.5285689884228546
1103	70-71	-0.5785041948640348
1103	72-73	-0.6290825525809645
1103	74-75	-0.661432593045209
1103	76-77	-0.7002105405992296
1103	78-79	-0.7970392782974116
1103	80-81	-0.7752123147198198
1103	82-83	-0.9603753476206798
1103	84-85	-0.824388735885254
1103	86-87	-1.4873291964423707
1103	88-89	-1.0388586813985938
1103	90-91	-1.0828500357685513
1103	92-93	-0.9818981719990347
1103	94-95	-1.3798285463198532
1103	96-97	-1.2241531023386862
1103	98-99	-1.1601945162393115
1103	100	-0.8645317522245257
1104	1	0.12302754037636632
1104	2	0.035841274398357825
1104	3	0.11165148277276415
1104	4	0.12694205570893047
1104	5	0.11469662482404885
1104	6	0.31168160753401253
1104	7	0.12231963412504854
1104	8	0.09446334519549993
1104	9	0.199951591353404
1104	10-11	0.04101982663751613
1104	12-13	0.16570374196822257
1104	14-15	0.015159031269583068
1104	16-17	0.13038527751784557
1104	18-19	0.20204644002549088
1104	20-21	-0.12294561813215665
1104	22-23	-0.05786451684850391
1104	24-25	0.15600735592504122
1104	26-27	0.034124562916098
1104	28-29	0.1736959676115717
1104	30-31	0.020968788039276376
1104	32-33	0.019736736320503212
1104	34-35	-0.1484903687581749
1104	36-37	-0.14534090376057662
1104	38-39	-0.11059779266754077
1104	40-41	-0.049052116871791895
1104	42-43	-0.25396853106280304
1104	44-45	0.0018788775331231022
1104	46-47	0.05164929199688828
1104	48-49	0.055581371409694214
1104	50-51	-0.2371110632979878
1104	52-53	-0.20406459368215124
1104	54-55	0.040038354511807484
1104	56-57	0.11994651861128602
1104	58-59	0.051615743674211956
1104	60-61	0.09676002465806732
1104	62-63	0.3064589744281818
1104	64-65	-0.012761514395020157
1104	66-67	0.05769328755190273
1104	68-69	0.12066213098860601
1104	70-71	0.056564962312656064
1104	72-73	-0.0406116044757141
1104	74-75	-0.11931119842448012
1104	76-77	-0.14995321628542868
1104	78-79	-0.15824865187797243
1104	80-81	-0.25443956108604837
1104	82-83	-0.22281674499917514
1104	84-85	-0.3060371424617294
1104	86-87	-0.12619180878095548
1104	88-89	-0.11018532105533296
1104	90-91	-0.19866676164616948
1104	92-93	-0.320262408583293
1104	94-95	-0.26139092465351155
1104	96-97	-0.32719131368123655
1104	98-99	-0.37892930794653523
1104	100	0.039744718315997574
1105	1	-0.07204724094197346
1105	2	0.0023624787095108957
1105	3	-0.06341099770208558
1105	4	0.02536149494667228
1105	5	0.14514072432420733
1105	6	0.010621391976371797
1105	7	-0.12191166078279281
1105	8	-0.10604186801911908
1105	9	-0.18321773073344616
1105	10-11	-0.02743485549306257
1105	12-13	-0.009736306063643951
1105	14-15	-0.053702994269308135
1105	16-17	-0.24291945147309235
1105	18-19	-0.18115900358587567
1105	20-21	-0.1438914651330947
1105	22-23	-0.08088124990067058
1105	24-25	-0.12140644054480987
1105	26-27	-0.10622806126384887
1105	28-29	-0.2231358329976203
1105	30-31	-0.11520276967706167
1105	32-33	-0.22539027476978646
1105	34-35	-0.14431418358167036
1105	36-37	0.18130555437750218
1105	38-39	0.2091282206876599
1105	40-41	0.19723102706759477
1105	42-43	-0.09033826903530695
1105	44-45	-0.06062820033317706
1105	46-47	-0.09745328337019288
1105	48-49	0.1339528764081379
1105	50-51	0.10933926472451105
1105	52-53	0.14135749121319208
1105	54-55	0.04571114231249851
1105	56-57	0.2504484613635469
1105	58-59	0.4501882112624642
1105	60-61	0.269334024970469
1105	62-63	0.27513014164154725
1105	64-65	0.26952746756374424
1105	66-67	0.3736834078268174
1105	68-69	0.465867974865521
1105	70-71	0.4409929242776691
1105	72-73	0.14505815692697155
1105	74-75	0.18064633437776578
1105	76-77	0.12729705706664873
1105	78-79	0.1976125536046922
1105	80-81	0.21495891790489097
1105	82-83	0.05740135090835352
1105	84-85	0.08992725343018293
1105	86-87	0.08719194824809406
1105	88-89	0.23049752219677444
1105	90-91	0.10200246281493719
1105	92-93	0.14829233993279445
1105	94-95	0.12817825294724017
1105	96-97	0.13513003121098777
1105	98-99	0.011102127542375939
1105	100	0.11985200900640436
1106	1	-0.0437357493000583
1106	2	-0.05921361774339928
1106	3	0.048947322526231574
1106	4	0.06001697866732769
1106	5	-0.09808602155499102
1106	6	0.053494011232011474
1106	7	-0.25484427188420256
1106	8	-1.4168998096581191
1106	9	-0.025757191389281786
1106	10-11	-0.0322481818370548
1106	12-13	-0.07181912975750748
1106	14-15	-0.1099042872974465
1106	16-17	-0.1999012209413209
1106	18-19	-0.2526925815461496
1106	20-21	-0.19537844748500532
1106	22-23	-0.13874134658656345
1106	24-25	-0.05942263251871793
1106	26-27	-0.08867782537357982
1106	28-29	0.07032563825872273
1106	30-31	-0.2675156591872181
1106	32-33	-0.12880573286285824
1106	34-35	-0.03765567114646018
1106	36-37	-0.06401915876828213
1106	38-39	-0.2966275018354878
1106	40-41	0.011242326502632238
1106	42-43	0.04378835453042029
1106	44-45	-0.07187521530200769
1106	46-47	-0.07053267063793811
1106	48-49	-0.03140678344392711
1106	50-51	-0.12649858255838353
1106	52-53	-0.08757022734933173
1106	54-55	-0.07763908192887214
1106	56-57	-0.1349834216814756
1106	58-59	-0.17122829523179917
1106	60-61	-0.1085645130923183
1106	62-63	-0.18209625477059177
1106	64-65	-0.2352217898187483
1106	66-67	-0.17793171244809614
1106	68-69	-0.40332067910384595
1106	70-71	-0.20130074454404578
1106	72-73	-0.21145882134782568
1106	74-75	-0.13802219611323352
1106	76-77	-0.2379915444826537
1106	78-79	-0.24998136566841822
1106	80-81	-0.19348304144044093
1106	82-83	-0.13711085131658862
1106	84-85	-0.20123239477297616
1106	86-87	-0.29772854799513127
1106	88-89	-0.28187710649446984
1106	90-91	-0.2762327285182806
1106	92-93	-0.35558790935370865
1106	94-95	-0.44425927981529867
1106	96-97	-0.304517007055658
1106	98-99	-0.2933582370682615
1106	100	-0.27660206519709973
1107	1	0.10188783449401129
1107	2	0.07101841343579096
1107	3	-0.04944477391172697
1107	4	0.2876205316447624
1107	5	0.15877249380800862
1107	6	0.2286267946997853
1107	7	0.24021736139777516
1107	8	0.2994683585644893
1107	9	0.22735800846569987
1107	10-11	0.14091120364286525
1107	12-13	0.23678913635324506
1107	14-15	0.31101046843535585
1107	16-17	0.2570146224376302
1107	18-19	0.3368225095442128
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2316	100	0.2238716817577533
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	2561.0
1	257.0
2	153.0
3	95.0
4	81.0
5	98.0
6	97.0
7	429.0
8	1414.0
9	3091.0
10	5065.0
11	6467.0
12	6538.0
13	5708.0
14	5482.0
15	6808.0
16	10001.0
17	14957.0
18	19062.0
19	23914.0
20	30258.0
21	35085.0
22	31635.0
23	26887.0
24	23708.0
25	22630.0
26	23781.0
27	26195.0
28	30230.0
29	35485.0
30	41673.0
31	51634.0
32	65164.0
33	84133.0
34	115476.0
35	182210.0
36	199648.0
37	198906.0
38	148699.0
39	19686.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.15282353794197	17.652259116389246	13.115533799105838	18.079383546562948
2	14.409945980150846	24.519308423626754	35.37346280726407	25.697282788958336
3	25.519758345514216	30.746655864878154	29.35070680206211	14.382878987545519
4	13.746448284017362	20.037440832492248	33.02980943149227	33.186301451998126
5	9.92057358635374	40.753638212086244	33.866351501520306	15.459436700039705
6	26.996221012502293	31.900114867901912	17.872113735870414	23.231550383725384
7	19.426428402102083	38.42455591750414	22.54712508356928	19.6018905968245
8	27.593347871685168	28.310565830433987	22.327645619350438	21.768440678530407
9	27.870210599432195	17.680016462758005	21.63980258009778	32.80997035771202
10-11	23.838670405407967	27.76801072485663	23.477269103151507	24.916049766583896
12-13	29.988520503286704	26.57213838253625	24.73558537442869	18.703755739748356
14-15	28.15824887470788	25.278739260088184	27.931376402932965	18.631635462270967
16-17	19.178253647527203	30.236843769660698	31.401123135458658	19.18377944735344
18-19	20.555309371382492	28.576921937273752	33.65555530770673	17.212213383637028
20-21	21.38383604652518	25.79403935695772	31.944858308626333	20.87726628789077
22-23	19.05190250365786	28.033544121539183	29.890255858819064	23.024297515983893
24-25	21.649457079899822	29.113846934383186	27.472885621081485	21.763810364635503
26-27	21.621233840292792	24.812128943427957	31.04128535960654	22.52535185667271
28-29	20.20480835879649	26.58570170117649	33.11078454938366	20.098705390643364
30-31	22.76202361498867	26.997655544455945	30.92550902780159	19.3148118127538
32-33	17.532431214132238	28.230354005104303	34.084451993546715	20.152762787216744
34-35	16.4885651183266	28.238540569239245	33.57885749379616	21.694036818638
36-37	20.38389094804599	27.625181179563015	31.700647752308146	20.29028012008285
38-39	22.254322078446087	25.366990909245256	31.346408457760965	21.03227855454769
40-41	18.38222759103828	28.511376621854517	31.03938532679486	22.067010460312346
42-43	20.767824339017494	27.615382720340264	30.251156373989357	21.365636566652885
44-45	22.47688206349032	25.259722082143774	30.47000784550252	21.79338800886339
46-47	19.590448321212932	28.46253681973981	32.922414662586554	19.02460019646071
48-49	20.232176004218477	27.356015190776876	32.846992338032074	19.564816466972577
50-51	17.328206920657113	28.184118107221916	32.9836720427423	21.50400292937867
52-53	17.46300052337962	29.621888188533855	28.305848134677696	24.609263153408826
54-55	17.640434735269448	28.540082089728664	27.915702032997714	25.903781142004178
56-57	20.273685801720802	25.6184467386813	28.00523145492182	26.10263600467608
58-59	21.502354764548517	24.219398465449306	30.338623299267354	23.93962347073483
60-61	20.95812569282854	26.195260303795955	30.39517441802403	22.451439585351483
62-63	17.120884287553285	26.670109676856512	31.721644946400257	24.487361089189946
64-65	15.659999054428306	28.723438258496657	31.2437863620594	24.372776325015632
66-67	19.666938030762367	26.58875353980312	28.073831011755225	25.670477417679287
68-69	20.258568447017673	25.204346789521143	28.589204682428377	25.94788008103281
70-71	19.582455608639975	26.21260892806293	28.238150508483606	25.966784954813484
72-73	20.705136339881953	24.018781519448506	30.621091138732037	24.654991001937507
74-75	20.568760119323937	25.274483019516353	29.15327691265629	25.003479948503422
76-77	18.622268546577068	24.830535001898117	31.568508614775993	24.97868783674883
78-79	19.931017098209395	24.678270152840618	31.856507838829888	23.534204910120103
80-81	19.25874229722562	24.761620630100012	30.91748046566095	25.062156607013414
82-83	18.14517673157719	24.874219836653115	29.958772011320466	27.021831420449228
84-85	18.808625223827764	25.848697261650283	28.588279134573725	26.75439837994823
86-87	19.917332585665964	24.12772325296575	28.709361403714407	27.24558275765388
88-89	20.164168189640932	22.871288249742918	29.842358842930288	27.12218471768586
90-91	21.554748667346868	22.95829681468596	29.24910557063661	26.237848947330566
92-93	20.360947704965355	23.72425291975311	30.11356731754269	25.801232057738847
94-95	19.21691878474491	23.365827960297814	30.66742858208473	26.749824672872546
96-97	20.674971162478457	22.954800348447833	29.77264311535808	26.59758537371563
98-99	22.537445872671547	22.35461578545184	28.150139761298686	26.957798580577926
100	20.81330088176091	21.361420728774927	29.77211962321007	28.053158766254093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6557.0
1	4559.0
2	2471.0
3	2132.0
4	1606.5
5	1575.0
6	2236.5
7	3282.0
8	4484.5
9	5096.0
10	4197.5
11	3022.5
12	2769.5
13	2886.0
14	2974.5
15	2980.5
16	3169.5
17	3440.5
18	3734.5
19	4083.0
20	4451.5
21	5022.5
22	5765.0
23	6561.0
24	7744.5
25	9239.0
26	10824.5
27	12975.0
28	15529.0
29	17966.0
30	20322.0
31	21957.0
32	24296.0
33	25630.5
34	24673.5
35	23535.0
36	22227.0
37	21131.5
38	20167.0
39	19860.0
40	18560.0
41	16711.5
42	15994.0
43	14925.5
44	14803.0
45	17062.5
46	27033.0
47	59193.0
48	122763.0
49	171240.5
50	228210.0
51	202593.5
52	86978.0
53	34697.0
54	19671.0
55	12621.0
56	8848.0
57	6936.0
58	5711.5
59	4950.0
60	4606.0
61	4227.0
62	3737.0
63	3376.5
64	2836.5
65	1996.5
66	1534.5
67	1418.5
68	1269.5
69	1110.5
70	973.5
71	887.5
72	808.5
73	704.0
74	568.5
75	469.0
76	371.0
77	286.5
78	229.0
79	167.0
80	127.0
81	101.5
82	86.0
83	56.5
84	38.0
85	27.5
86	24.5
87	16.5
88	12.0
89	11.0
90	5.5
91	2.5
92	2.0
93	2.0
94	3.0
95	1.5
96	0.5
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14248193568186077
2	0.06689012085390852
3	0.2967875471452466
4	0.29353271504808515
5	0.2950604933794058
6	0.2476993651084656
7	0.24617158677714496
8	0.2443781078664642
9	0.25792883741383
10-11	0.23398257269899975
12-13	0.24218607808674328
14-15	0.21843908880643312
16-17	0.22644331875965656
18-19	0.24195359007980316
20-21	0.2448762964527644
22-23	0.23590890189936056
24-25	0.23444754871287996
26-27	0.23202303092621893
28-29	0.23986119801734226
30-31	0.22581227988367628
32-33	0.2380012939618215
34-35	0.2232881243797552
36-37	0.23189018063653888
38-39	0.23537750074064037
40-41	0.250954197205627
42-43	0.23653994077534085
44-45	0.220498268296474
46-47	0.22401880097299554
48-49	0.23182375549169887
50-51	0.22777182165645712
52-53	0.24417883243194413
54-55	0.25271446354388777
56-57	0.25032315832964674
58-59	0.24653692507376512
60-61	0.22976457600165798
62-63	0.25646748422734933
64-65	0.2469022633703853
66-67	0.2511866852125671
68-69	0.25507255618570873
70-71	0.2774246174243783
72-73	0.2854620599500217
74-75	0.26536845363591316
76-77	0.26330927414587224
78-79	0.2605526306350111
80-81	0.2526480383990477
82-83	0.2607519060695312
84-85	0.2670622948293339
86-87	0.25872593915191033
88-89	0.2660991302291535
90-91	0.26158222038003154
92-93	0.2429499672524036
94-95	0.26403995073911257
96-97	0.26158222038003154
98-99	0.2515188109367672
100	0.27500009963771727
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1505454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.047050198749265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.742691247292	16.362543252676197
2	8.29923735864329	3.4934893059740126
3	3.465387730434424	2.1880856856174935
4	1.972271941615405	1.6604202624305646
5	1.3689787930860713	1.4406482689552864
6	0.9891437509950878	1.2491134908583656
7	0.7548293779452452	1.112085226637298
8	0.6046662869774097	1.0181153356403896
9	0.47189545679370987	0.8938806630929034
>10	3.7701913944750363	15.699791005746967
>50	0.34157630836705627	4.870966084588156
>100	0.17174222180362295	6.973228464724898
>500	0.0197185513922618	2.9362476875718255
>1k	0.014629892968452303	7.7329779518761566
>5k	0.00795102878720234	10.871379247483247
>10k+	0.005088658423809497	21.497028066126276
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	48484	3.2205567224239333	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	42158	2.800351256165914	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	25450	1.6905199361787207	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	23776	1.5793242437165134	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	19514	1.2962202764083128	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	17861	1.1864195119877459	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	17724	1.177319267144662	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	16286	1.0817999088647012	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15629	1.0381585887048028	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	15454	1.0265341883577976	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	15147	1.006141668891909	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	15118	1.004215339691548	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14986	0.9954472205726644	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	13245	0.8798010434061752	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	10308	0.6847103930110119	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	10007	0.6647164244141635	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8639	0.5738468262730047	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	8534	0.5668721860648017	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7892	0.5242272430775036	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	7795	0.5177840040280207	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	7754	0.5150605730895796	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	6920	0.45966200229299603	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	6838	0.4542151404161137	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	6689	0.4443177938349495	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	6615	0.4394023331167874	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	6602	0.43853880623386704	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	6565	0.43608107587478595	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	6555	0.4354168244263857	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	6554	0.4353503992815456	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	6454	0.42870788479754285	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	6247	0.41495787981565696	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	6071	0.403267054323812	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	5963	0.39609313868108886	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	5938	0.3944325100600882	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	5720	0.379951828484962	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	5593	0.3715158350902784	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	5527	0.3671317755308366	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	5366	0.356437327211592	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	5283	0.35092404018986967	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	5260	0.349396261858549	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	5035	0.3344506042695426	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	4917	0.3266124371784193	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	4796	0.31857499465277583	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	4635	0.3078805463335313	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4478	0.29745179859364684	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	4198	0.2788527580384389	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	4093	0.27187811783023597	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	3957	0.26284429813199206	No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	3840	0.25507255618570873	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	3830	0.2544083047373085	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	3790	0.25175129894370735	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3666	0.24351458098354384	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	3558	0.2363406653408208	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	3556	0.23620781505114072	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	3163	0.21010273312900957	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	3067	0.20372591922436684	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	3063	0.20346021864500677	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	2839	0.1885809862008404	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	2812	0.18678750729015964	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2731	0.18140707055811733	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	2634	0.17496383150863462	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	2505	0.16639498782427095	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	2290	0.15211358168366484	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	2182	0.1449396660409418	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	2125	0.14115343278506018	No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	2064	0.13710149894981846	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	1940	0.12886478098965495	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1930	0.12820052954125466	No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC	1776	0.1179710572358903	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT	1715	0.11391912340064858	No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	1682	0.11172709362092764	No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA	1673	0.11112926731736737	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT	1510	0.10030196870844277	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3285028968005664E-4	0.0
10-11	0.0	0.0	0.0	1.9927543452008496E-4	0.0
12-13	0.0	0.0	0.0	2.9891315178012746E-4	0.0
14-15	0.0	0.0	0.0	5.314011587202266E-4	0.0
16-17	0.0	0.0	0.0	8.303143105003541E-4	0.0
18-19	0.0	0.0	0.0	0.0013617154692205807	0.0
20-21	0.0	0.0	0.0	0.006808577346102903	0.0
22-23	0.0	0.0	0.0	0.021256046348809066	0.0
24-25	0.0	0.0	0.0	0.04570049964993948	0.0
26-27	0.0	0.0	0.0	0.09020534669275845	0.0
28-29	0.0	0.0	0.0	0.23889803341716187	0.0
30-31	0.0	0.0	0.0	0.5201753092422619	0.0
32-33	0.0	0.0	0.0	0.9972406994833451	0.0
34-35	0.0	0.0	0.0	1.8256951059281783	0.0
36-37	0.0	0.0	0.0	2.889028824527352	0.0
38-39	0.0	0.0	0.0	4.084847494509962	0.0
40-41	0.0	0.0	0.0	5.501729046520186	0.0
42-43	0.0	0.0	0.0	6.657792267316038	0.0
44-45	0.0	0.0	0.0	7.689640467261039	0.0
46-47	0.0	0.0	0.0	8.9092725516688	0.0
48-49	0.0	0.0	0.0	10.067793502823733	0.0
50-51	0.0	0.0	0.0	11.147334956763874	0.0
52-53	0.0	6.642514484002832E-5	0.0	12.19356420056674	0.0
54-55	0.0	6.642514484002832E-5	0.0	13.338866547898508	0.0
56-57	0.0	6.642514484002832E-5	0.0	14.593670746499063	0.0
58-59	0.0	6.642514484002832E-5	0.0	15.672282248411443	0.0
60-61	0.0	6.642514484002832E-5	0.0	16.590178112383374	0.0
62-63	0.0	6.642514484002832E-5	0.0	17.42095739889761	0.0
64-65	0.0	6.642514484002832E-5	0.0	18.35795049201105	0.0
66-67	0.0	6.642514484002832E-5	0.0	19.35897742475027	0.0
68-69	0.0	6.642514484002832E-5	0.0	20.549349232856002	0.0
70-71	0.0	9.963771726004248E-5	0.0	22.03092887594041	0.0
72-73	0.0	1.3285028968005664E-4	0.0	23.184833279528966	0.0
74-75	0.0	1.9927543452008496E-4	0.0	24.242753348823676	0.0
76-77	0.0	1.9927543452008496E-4	0.0	25.323889006239977	0.0
78-79	0.0	2.657005793601133E-4	0.0	26.45447818398968	0.0
80-81	0.0	2.657005793601133E-4	0.0	27.57769417066214	0.0
82-83	0.0	2.657005793601133E-4	0.0	28.855780382529126	0.0
84-85	0.0	2.657005793601133E-4	0.0	30.096768151002955	0.0
86-87	0.0	2.9891315178012746E-4	0.0	31.199425555347425	0.0
88	0.0	3.321257242001416E-4	0.0	32.1278498047765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCA	1410	0.0	61.042973	1
TACCCAT	1595	0.0	54.235943	2
ACCCATG	1780	0.0	48.59906	3
AAGACGT	100	7.057679E-10	42.305832	5
AGACGTT	100	7.075869E-10	42.29456	6
GACGTTA	105	1.1423253E-9	40.279194	7
CGCCGGT	85	3.952464E-7	38.711216	94
GCGTCAG	70	2.1762997E-4	33.595078	1
GTACCTA	85	1.746701E-5	33.19984	1
CAAGACG	130	9.173164E-9	32.545113	4
GTCCTAA	145	7.385097E-10	32.436626	1
CGTGTCA	90	2.5920075E-5	31.33765	94
CGTTAAC	145	2.6653652E-8	29.167692	9
CGTCAGA	85	6.80495E-4	27.655476	2
TAAGGTG	70	0.00882376	26.86532	3
GATGACC	70	0.008843846	26.852797	8
CCTAAAT	195	6.039045E-10	26.520891	3
TCAAGAC	185	1.0009899E-8	25.41314	3
GCCGTCT	225	1.1641532E-10	25.07012	94
ATGGGCA	790	0.0	23.800747	5
>>END_MODULE