##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522953_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1505454 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97121599198647 33.0 31.0 34.0 30.0 34.0 2 32.019828569986196 33.0 31.0 34.0 30.0 34.0 3 32.164994081519595 33.0 31.0 34.0 30.0 35.0 4 35.29468651981396 37.0 35.0 37.0 32.0 37.0 5 35.239829313947816 36.0 35.0 37.0 32.0 37.0 6 35.47860711785282 36.0 35.0 37.0 33.0 37.0 7 35.27994146616237 36.0 35.0 37.0 32.0 37.0 8 35.34757156312979 36.0 35.0 37.0 33.0 37.0 9 36.48063175626754 37.0 35.0 39.0 33.0 39.0 10-11 36.31004633818104 37.0 35.0 39.0 32.0 39.0 12-13 36.34168662742269 37.0 35.0 39.0 32.0 39.0 14-15 37.225817593895265 38.0 36.5 40.0 32.0 41.0 16-17 36.99167892210589 37.5 36.0 40.0 31.5 41.0 18-19 37.02626549864692 38.0 36.0 40.0 31.5 41.0 20-21 37.1919620260732 38.0 36.0 40.0 32.5 41.0 22-23 37.10913817360078 38.0 35.5 40.0 32.0 41.0 24-25 37.01560094164285 38.0 36.5 40.0 32.0 41.0 26-27 36.56119084342663 37.0 35.5 40.0 31.5 41.0 28-29 36.51632696847595 38.0 35.5 40.0 31.0 41.0 30-31 36.17126760432401 36.5 35.0 40.0 30.5 41.0 32-33 35.8456133498599 37.0 35.0 40.0 30.0 41.0 34-35 35.6409591392364 37.0 35.0 40.0 30.0 41.0 36-37 35.42426238197912 37.0 35.0 40.0 29.0 41.0 38-39 35.23785250163738 36.5 34.5 40.0 27.5 41.0 40-41 35.31554700442524 37.0 35.0 40.0 28.0 41.0 42-43 35.2231117656202 37.5 35.0 40.0 27.5 41.0 44-45 34.809651440694964 37.0 35.0 40.0 25.0 41.0 46-47 34.367850495598006 37.0 34.0 40.0 23.5 41.0 48-49 33.96642175715765 37.0 33.0 40.0 21.5 41.0 50-51 33.66790748837228 36.5 33.0 40.0 18.5 41.0 52-53 33.69162691121748 37.0 33.0 40.0 16.5 41.0 54-55 33.55313480186044 37.5 33.0 40.0 14.5 41.0 56-57 33.185193303814 37.0 33.0 40.0 12.5 41.0 58-59 32.97915147191478 36.5 32.5 40.0 12.5 41.0 60-61 32.579287045635404 36.5 32.0 39.0 11.5 41.0 62-63 32.19128050408714 36.0 31.0 39.0 10.5 40.5 64-65 32.025970903129554 36.5 31.0 38.5 10.0 40.0 66-67 31.357433040132744 35.5 30.5 37.0 10.0 40.0 68-69 31.09292711700258 35.5 29.5 37.0 10.0 39.5 70-71 30.490167750060778 35.0 29.0 37.0 9.0 39.0 72-73 30.138140388215117 35.0 29.0 36.0 9.0 38.5 74-75 29.902371643371367 35.0 28.0 36.5 8.0 38.0 76-77 28.318594922196226 32.5 25.0 35.5 8.0 36.0 78-79 29.204382199655385 34.0 26.0 36.0 8.0 37.0 80-81 29.30360343125728 34.0 27.0 36.0 8.0 37.5 82-83 29.227736948455416 34.0 26.5 36.0 7.5 37.0 84-85 28.923160388826226 34.0 26.0 35.5 7.0 36.5 86-87 28.821792960794554 34.0 26.0 35.0 7.0 36.0 88-89 28.710951646480062 34.0 25.5 35.0 7.0 37.0 90-91 28.434844571803588 34.0 25.0 35.0 7.0 36.5 92-93 28.143683898677743 33.0 24.0 35.0 7.0 36.5 94-95 28.29253833062983 34.0 24.5 35.0 7.0 36.5 96-97 28.06015760029865 34.0 24.0 35.0 7.0 36.5 98-99 27.892618107228785 33.0 24.0 35.0 7.0 36.0 100 26.709227913971468 32.0 19.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.15199445794212707 1101 2 -0.21854690246298958 1101 3 -0.1480543058503514 1101 4 -0.14040654529907215 1101 5 -0.13370300140261548 1101 6 -0.035392581099571885 1101 7 -0.08840877896447807 1101 8 -0.22204798484526123 1101 9 -0.1270912586621833 1101 10-11 -0.15045809329809146 1101 12-13 -0.1305073262367742 1101 14-15 -0.2755790784659169 1101 16-17 -0.28769884136956847 1101 18-19 -0.19755987537752873 1101 20-21 -0.22147670305145795 1101 22-23 -0.8145388887814846 1101 24-25 -0.27959194372969165 1101 26-27 0.002474359575643348 1101 28-29 -0.32350007734729047 1101 30-31 -0.3349907487710482 1101 32-33 -0.3539391820857958 1101 34-35 -0.3276628731466431 1101 36-37 -0.22035832712133185 1101 38-39 -0.2240437621135598 1101 40-41 -0.2904501626288791 1101 42-43 -0.3347215526189231 1101 44-45 -0.5848176705444317 1101 46-47 -0.2049703639250211 1101 48-49 -0.21375782578732583 1101 50-51 -0.11304550768161903 1101 52-53 -0.15822439548530554 1101 54-55 -0.2647868146034469 1101 56-57 -0.5639331084193699 1101 58-59 -0.352562616706102 1101 60-61 -0.3708778924222855 1101 62-63 -0.3598855220230561 1101 64-65 -0.3233901172338385 1101 66-67 -0.26412399220883387 1101 68-69 -0.16034685657127667 1101 70-71 -0.11438696999427478 1101 72-73 -0.1085868002465844 1101 74-75 -0.24877664652074571 1101 76-77 -0.21219930846876878 1101 78-79 -0.42271760917060774 1101 80-81 -0.185608681246336 1101 82-83 -0.2643776532303086 1101 84-85 -0.3803551326312409 1101 86-87 -0.4112256201198363 1101 88-89 -0.48573777955659736 1101 90-91 -0.5375101859699178 1101 92-93 -0.38538839686998116 1101 94-95 -0.47053474496607706 1101 96-97 -0.7695882599845838 1101 98-99 -0.9332730045540671 1101 100 -0.26738518918870824 1102 1 -0.07416517023818514 1102 2 -0.23979839470333886 1102 3 -0.22928192224510013 1102 4 -0.18041450391427816 1102 5 -0.26496842121956377 1102 6 -0.21351832721974517 1102 7 -0.09790340689922061 1102 8 -0.22809255309039145 1102 9 -0.2259472077270459 1102 10-11 -0.34282976062798554 1102 12-13 -0.33070330713609764 1102 14-15 -0.38702157747108856 1102 16-17 -0.4420233539043963 1102 18-19 -0.6437287969851653 1102 20-21 -0.5940095323391645 1102 22-23 -0.3737499910496922 1102 24-25 -0.2406275585327151 1102 26-27 -0.3210950793419798 1102 28-29 -0.39299967941653335 1102 30-31 -0.45158645111884255 1102 32-33 -0.3645629385521687 1102 34-35 -0.4407647434212123 1102 36-37 -0.40669636930199715 1102 38-39 -0.255548935213433 1102 40-41 -0.2907088176229067 1102 42-43 -0.2934481741867714 1102 44-45 -0.22992212736894402 1102 46-47 -0.1543525764200453 1102 48-49 -0.23838973983427536 1102 50-51 -0.036269741664909816 1102 52-53 -0.008201514466605886 1102 54-55 0.05414374647892828 1102 56-57 -0.011221210289640737 1102 58-59 0.07552333633807251 1102 60-61 -0.11392803169805177 1102 62-63 -0.17342014199918054 1102 64-65 -0.23123034803368014 1102 66-67 -0.25019243629956023 1102 68-69 0.02313702723294142 1102 70-71 0.052843431915793104 1102 72-73 -0.082339287313836 1102 74-75 -0.020320617869728608 1102 76-77 -0.11511514611301976 1102 78-79 -0.18778824188051857 1102 80-81 -0.22976407320813763 1102 82-83 -0.30679110328999926 1102 84-85 -0.22536806538094112 1102 86-87 -0.24235375382457391 1102 88-89 -0.3156303382513812 1102 90-91 -0.4847356137454888 1102 92-93 -0.346692614936039 1102 94-95 -0.5717713148029269 1102 96-97 -0.4354786300601923 1102 98-99 -0.3529437168501275 1102 100 -0.34581933164792034 1103 1 -0.025522040281270364 1103 2 -0.18627862470220435 1103 3 -0.2701227263663135 1103 4 -0.029165594957483165 1103 5 -0.03812637246409878 1103 6 -0.11207964136264792 1103 7 -0.0492826445706811 1103 8 -0.015859548014425684 1103 9 -0.11876302372795067 1103 10-11 -0.23336010206734414 1103 12-13 -0.10280848867176928 1103 14-15 -0.12206832084380181 1103 16-17 -0.22716886278284676 1103 18-19 -0.24774622186472328 1103 20-21 -0.2824739499159463 1103 22-23 -0.25048795577445304 1103 24-25 -0.03420268095819523 1103 26-27 -0.05537411600302278 1103 28-29 -0.07595470159527906 1103 30-31 -0.15250069064930472 1103 32-33 -0.23794575902064707 1103 34-35 -0.22544252033741685 1103 36-37 -0.3710856137533298 1103 38-39 -0.21118075609744125 1103 40-41 -0.17838480115238298 1103 42-43 -0.30894098771630496 1103 44-45 -0.09711781329961156 1103 46-47 -0.26291137544739485 1103 48-49 -0.3877381459667859 1103 50-51 -0.460698969494608 1103 52-53 -0.41805997211424994 1103 54-55 -1.1079547048858984 1103 56-57 -0.500839399843862 1103 58-59 -0.33979928439244844 1103 60-61 -0.35923977679059504 1103 62-63 -0.42076932950750745 1103 64-65 -0.4266528377395993 1103 66-67 -0.4493982860805552 1103 68-69 -0.4804161947990728 1103 70-71 -0.43869613691586196 1103 72-73 -0.5232967013376424 1103 74-75 -0.6418081546271992 1103 76-77 -0.5159820656830014 1103 78-79 -0.2298224832401523 1103 80-81 -1.3933850144777011 1103 82-83 -0.3599920103221841 1103 84-85 -0.2929361971769744 1103 86-87 -0.2569608092704456 1103 88-89 -0.2271372901102815 1103 90-91 -0.5324089827477323 1103 92-93 -0.42694468319607637 1103 94-95 -0.4154228351057725 1103 96-97 -0.4118124365931308 1103 98-99 -0.4799786739738714 1103 100 -0.46896008467152583 1104 1 0.041544503096837104 1104 2 -0.1839852141512992 1104 3 -0.11051372143477067 1104 4 0.02137511054507968 1104 5 -0.040504949454565065 1104 6 -0.33689014603463363 1104 7 -0.1441433568865591 1104 8 -0.06795707575435728 1104 9 -0.2063932067141323 1104 10-11 -0.09411271992147618 1104 12-13 -0.019393284028986102 1104 14-15 -0.23470651353084548 1104 16-17 -0.1421590686423002 1104 18-19 -0.28633016758531227 1104 20-21 -0.30077865110340696 1104 22-23 -0.08912492774253167 1104 24-25 -0.14406434632709164 1104 26-27 -0.16859503977499912 1104 28-29 -0.22443148968496018 1104 30-31 -0.10427443383598245 1104 32-33 -0.2115000587091771 1104 34-35 -0.2121190419778074 1104 36-37 -0.3221704212771712 1104 38-39 -0.25765983354212807 1104 40-41 -0.06280202951199243 1104 42-43 -0.13376376041113502 1104 44-45 -0.16532903418079314 1104 46-47 -0.26420534769125226 1104 48-49 -0.3184960563067989 1104 50-51 -0.2344943713179859 1104 52-53 -0.2617308889918064 1104 54-55 -0.2099045094086378 1104 56-57 -0.1270337577700147 1104 58-59 -0.12134711345934335 1104 60-61 -0.21573584696773906 1104 62-63 -0.08956287591916379 1104 64-65 -0.37478825035072205 1104 66-67 -0.25137845649454604 1104 68-69 -0.2625039182595863 1104 70-71 -0.1494417589553123 1104 72-73 0.0898730211819867 1104 74-75 -0.04145927639087432 1104 76-77 -0.0476376201570794 1104 78-79 -0.16315186241736157 1104 80-81 -0.31647312929828786 1104 82-83 -0.138060688944595 1104 84-85 -0.8549350839299414 1104 86-87 -0.27521709739256295 1104 88-89 -0.22125320163451434 1104 90-91 -0.3289346989569317 1104 92-93 -0.4150495169998507 1104 94-95 -0.22714391704399972 1104 96-97 -0.4365261658287416 1104 98-99 -0.35229086169692536 1104 100 -1.4081887606172785 1105 1 -0.03424040786848792 1105 2 -0.17151450187388662 1105 3 -0.2621313799789746 1105 4 -0.18049491054208033 1105 5 -0.20335444961076377 1105 6 -0.1894279271967747 1105 7 -0.2232099571677182 1105 8 -0.2750720398280819 1105 9 -0.20425129793562036 1105 10-11 -0.2332857810742368 1105 12-13 -0.27460771897118974 1105 14-15 -0.33965369800445444 1105 16-17 -0.32677959738332163 1105 18-19 -0.3319990015434584 1105 20-21 -0.32672952583005355 1105 22-23 -0.33244635073533857 1105 24-25 -0.3675853163364664 1105 26-27 -0.5112811632520433 1105 28-29 -0.2416382707689948 1105 30-31 -0.4918488734642281 1105 32-33 -0.31141463618496346 1105 34-35 -0.38739532282754396 1105 36-37 -0.2923796820550635 1105 38-39 -0.21809260845621736 1105 40-41 -0.333957771858131 1105 42-43 -0.2436415329822026 1105 44-45 -0.36164756745164794 1105 46-47 -0.16142716313954253 1105 48-49 -0.21894928381382783 1105 50-51 -0.05252145269879804 1105 52-53 -0.04491192616768558 1105 54-55 -0.7763344336510656 1105 56-57 -0.11500821887592139 1105 58-59 -0.17663232706447474 1105 60-61 0.014190836100048898 1105 62-63 0.05839503762348386 1105 64-65 0.20691302464146943 1105 66-67 0.2061091938490982 1105 68-69 0.2878023825057987 1105 70-71 0.13877945121338442 1105 72-73 0.03473180554624733 1105 74-75 0.10274029015083386 1105 76-77 0.1074018206426679 1105 78-79 0.0841785616688604 1105 80-81 0.28572784305410437 1105 82-83 0.23317266586640173 1105 84-85 0.03240505391760706 1105 86-87 0.12752008434751616 1105 88-89 0.05272319246107671 1105 90-91 -0.13230522769795172 1105 92-93 0.06882490700827049 1105 94-95 -0.04431604098964215 1105 96-97 -0.006415702301719506 1105 98-99 0.3946270936295342 1105 100 0.6155333184205212 1106 1 -0.029222060847693854 1106 2 -0.07713043757194882 1106 3 -0.16252161819902256 1106 4 -0.06268257435476698 1106 5 -0.16304924837597667 1106 6 -0.057692823230325985 1106 7 0.05950889658031855 1106 8 -0.10459035772662162 1106 9 -0.06533581072029193 1106 10-11 -0.13176559935136112 1106 12-13 -0.17630030444500733 1106 14-15 -0.2029398532843132 1106 16-17 -0.11062257018313204 1106 18-19 -0.2242245242879406 1106 20-21 -0.13828057714346897 1106 22-23 0.004208084584128358 1106 24-25 0.07957875844948603 1106 26-27 0.1065905823359401 1106 28-29 0.07333406308853796 1106 30-31 -0.09319898121657388 1106 32-33 0.1192872101660214 1106 34-35 -0.01660960438150738 1106 36-37 -1.3435617702128866E-4 1106 38-39 -0.09449775727094334 1106 40-41 -0.12382788659981969 1106 42-43 -0.03547215794579728 1106 44-45 0.009800973523361733 1106 46-47 -0.15625971017198736 1106 48-49 0.0869322468519016 1106 50-51 0.17869178045397405 1106 52-53 -0.14820623567901237 1106 54-55 -0.27350957489399974 1106 56-57 -0.42524061447263506 1106 58-59 -0.3833374351080394 1106 60-61 -0.19435246869347367 1106 62-63 -0.1936930280763285 1106 64-65 -0.16124120682221488 1106 66-67 -0.1913758081894592 1106 68-69 -0.22494734083277734 1106 70-71 -0.2983094881540822 1106 72-73 -0.35851416102140377 1106 74-75 -0.2811737409517363 1106 76-77 -0.14811335205230236 1106 78-79 -0.18071397720935067 1106 80-81 -0.15347187737224388 1106 82-83 -0.13839702369035933 1106 84-85 -0.061680798490801436 1106 86-87 0.06733370191406252 1106 88-89 -0.10001138730477876 1106 90-91 -0.08258887846386642 1106 92-93 0.05255954501863869 1106 94-95 0.10972554559082326 1106 96-97 0.08111997246093594 1106 98-99 -0.12113620067997388 1106 100 -0.11584765892236248 1107 1 0.015140759781328228 1107 2 -0.07352398420477613 1107 3 -0.09572428293209612 1107 4 -0.038492870738345175 1107 5 0.021159489048109492 1107 6 -0.14441855512554724 1107 7 -0.2820527820202443 1107 8 -0.1642137602463336 1107 9 -0.21123946339861988 1107 10-11 -0.1888862827556963 1107 12-13 -0.24255087092738137 1107 14-15 -0.3430370616591958 1107 16-17 -0.11621488415032388 1107 18-19 -0.13329707935002233 1107 20-21 -0.18363292917827323 1107 22-23 -0.21186891169974587 1107 24-25 -0.08983640515062064 1107 26-27 -0.035218535764308 1107 28-29 -0.143444691556617 1107 30-31 -0.19248883891620494 1107 32-33 -0.25858394908350135 1107 34-35 -0.07984965695106894 1107 36-37 -0.08113181833599725 1107 38-39 -0.019817253328220374 1107 40-41 -0.1020525308488871 1107 42-43 -0.16635065880685573 1107 44-45 -0.10140857963533989 1107 46-47 -0.15739969929553155 1107 48-49 -0.2840500335795326 1107 50-51 -0.2982059354891078 1107 52-53 -0.1367308889918064 1107 54-55 -0.19707864042468515 1107 56-57 -0.3514655759518348 1107 58-59 -0.15729714688180962 1107 60-61 -0.073565058197687 1107 62-63 -0.20589392538440165 1107 64-65 -0.13851234794430667 1107 66-67 -0.2538642519490928 1107 68-69 -0.1837731361740289 1107 70-71 -0.11825442606761527 1107 72-73 -0.1946983357698855 1107 74-75 -0.20009062665825184 1107 76-77 -0.18985018700199774 1107 78-79 -0.0038520629521237026 1107 80-81 0.07035006588888137 1107 82-83 0.16727437121583222 1107 84-85 0.2025306580486692 1107 86-87 0.09913534244700983 1107 88-89 0.11869499355264779 1107 90-91 0.1316685764441381 1107 92-93 0.3280779896846404 1107 94-95 0.190783054881134 1107 96-97 0.027816247272863137 1107 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0.0 11 3.0 12 33.0 13 183.0 14 818.0 15 2422.0 16 5352.0 17 10179.0 18 15579.0 19 20882.0 20 26383.0 21 31954.0 22 32410.0 23 29439.0 24 28396.0 25 27608.0 26 28629.0 27 31290.0 28 33623.0 29 37780.0 30 43612.0 31 50713.0 32 61014.0 33 76345.0 34 102432.0 35 176368.0 36 261612.0 37 201106.0 38 151982.0 39 17260.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.293525111011576 18.655327824731295 13.82969674335027 19.22145032090686 2 13.7464342987576 24.862453946485584 35.16296267755432 26.228149077202495 3 24.770002935991403 30.241508541609374 30.798217680513652 14.190270841885571 4 13.090181068903695 20.886659063176502 32.13699035968671 33.886169508233095 5 9.542595237304461 41.458235079212194 34.288883722475006 14.710285961008337 6 27.002352778630232 32.16524716132144 17.490072762103658 23.342327297944674 7 18.61637751801118 38.02261643331513 22.104361873561064 21.256644175112623 8 27.7226670492755 28.942896959986818 22.473353553147422 20.861082437590255 9 26.42651319801203 17.343206766862355 21.43526138958746 34.79501864553816 10-11 23.561239188058615 27.588862757966286 23.88725390721135 24.96264414676375 12-13 29.63683308191366 26.127054284729294 25.105367095973236 19.130745537383813 14-15 27.832503479407556 25.092623655985413 27.58577279385631 19.489100070750723 16-17 19.293085931233627 30.204217331852917 31.163311757002397 19.339384979911063 18-19 20.7174449426701 28.389385656880133 33.16133643450296 17.731832965946808 20-21 21.163258148426646 25.854631590414524 31.207603869688942 21.77450639146989 22-23 18.74318930841402 28.54038887492108 29.201356010562986 23.515065806101916 24-25 21.913789782049832 29.455224227608795 26.740047429569746 21.890938560771627 26-27 22.14205279644436 25.138368937327886 30.62143946213664 22.09813880409111 28-29 20.137146380043752 26.27940286450185 32.81644348159413 20.767007273860276 30-31 22.783470979948238 27.215100737126853 30.727447744774476 19.273980538150433 32-33 18.098258440830733 27.976150341466234 33.982010121922094 19.94358109578094 34-35 16.06205053206242 28.345771982928564 33.866884914617906 21.725292570391108 36-37 20.550731559716336 27.800929931621894 31.481231144704708 20.16710736395706 38-39 21.981506514901405 25.217069274177213 31.294112176538384 21.507312034383002 40-41 18.112442489374732 28.361324162633338 31.37725243029307 22.148980917698857 42-43 21.03028839558759 27.34083305128291 30.685713014055576 20.94316553907392 44-45 22.073899634803144 25.417435451165094 30.59363172413106 21.915033189900704 46-47 19.658128459432195 28.234248341349357 32.71517115999082 19.39245203922763 48-49 20.21215353476835 27.10240977262905 32.68970195328343 19.99573473931916 50-51 16.953656727399803 28.092970230033853 32.94570823712464 22.0076648054417 52-53 16.887821534863946 29.431700414540817 28.58434975552721 25.096128295068027 54-55 17.060871067062134 28.318115843350082 27.56269949083131 27.058313598756474 56-57 20.20016022383509 25.776873031246307 27.251636448663934 26.771330296254668 58-59 20.66799396537464 24.93956406408501 30.078262793186532 24.31417917735381 60-61 20.393791352758523 27.639560214731272 28.857827717445637 23.108820715064567 62-63 15.986837167068208 28.922927415106837 29.957002918062425 25.13323249976253 64-65 14.679492949120545 30.878165705215814 29.1511411368225 25.29120020884114 66-67 19.153245359170263 28.312548739838533 25.9813064867328 26.552899414258402 68-69 20.120229631954512 26.51528361074162 26.34314267637139 27.021344080932476 70-71 19.955076599943606 27.59564157330874 25.457944012145195 26.991337814602463 72-73 21.32123424757357 25.37958274295986 27.59533493689065 25.703848072575912 74-75 21.480820387661037 26.121234002596715 25.83954086775834 26.558404741983903 76-77 19.352163330596454 26.173702838648992 28.529810730162275 25.944323100592282 78-79 20.527840828698345 25.833816594559444 28.736240340587898 24.902102236154317 80-81 19.49146237613371 25.76827322521977 27.712802915266753 27.027461483379767 82-83 18.154976108290043 26.008136499764024 27.03677367092711 28.80011372101883 84-85 19.272138140709373 26.460219050709405 25.46350743514981 28.804135373431407 86-87 20.2688035263898 24.874206964168167 25.81505584048347 29.041933668958563 88-89 20.23862624510072 23.586494541002843 26.85827265659017 29.31660655730627 90-91 21.30332140774229 23.739410115009107 26.49982131541085 28.457447161837756 92-93 19.709295161661554 24.580912813458085 27.579263134575864 28.13052889030449 94-95 18.448687239862526 24.381150138097876 28.14762191338958 29.022540708650013 96-97 19.585762753521863 24.167847125465194 27.672389885197962 28.574000235814985 98-99 21.496859235355913 23.240097366527053 26.094269105058938 29.168774293058103 100 19.370474362832134 23.39531475949732 27.69787924017454 29.536331637496005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4965.0 1 3138.5 2 1503.5 3 1733.0 4 1462.5 5 1415.5 6 2053.0 7 3041.0 8 4097.0 9 4676.0 10 3899.5 11 2761.0 12 2532.5 13 2641.0 14 2705.5 15 2731.0 16 2869.5 17 3060.5 18 3312.5 19 3587.0 20 3771.5 21 3997.5 22 4543.5 23 5036.5 24 5552.5 25 6584.0 26 7789.5 27 9377.0 28 11612.5 29 13928.0 30 16373.0 31 18694.5 32 21747.0 33 24278.5 34 24747.5 35 24819.5 36 24817.5 37 24405.0 38 23605.0 39 23432.0 40 22395.5 41 20347.0 42 19273.5 43 18181.5 44 17802.0 45 19240.5 46 26040.0 47 55085.0 48 104982.5 49 133588.5 50 189106.0 51 189033.0 52 116659.5 53 91805.0 54 59851.5 55 23193.0 56 9614.5 57 6117.5 58 4978.0 59 4130.0 60 3612.5 61 3318.0 62 2729.0 63 2228.5 64 1736.0 65 979.5 66 622.0 67 627.0 68 548.0 69 425.5 70 416.5 71 416.5 72 351.5 73 295.5 74 290.0 75 222.5 76 142.5 77 107.5 78 66.5 79 27.5 80 15.0 81 8.0 82 6.5 83 6.0 84 6.0 85 4.0 86 3.0 87 2.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043574895015058575 2 0.009299520277603964 3 0.0 4 3.321257242001416E-4 5 2.657005793601133E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.321257242001416E-5 12-13 1.9927543452008498E-4 14-15 0.011225849477964787 16-17 0.0015609909037406654 18-19 3.321257242001416E-5 20-21 6.642514484002832E-4 22-23 5.646137311402407E-4 24-25 0.004251209269761812 26-27 0.01587560961676677 28-29 0.013351454112845693 30-31 0.009963771726004248 32-33 0.0071407030703030455 34-35 0.008801331691303753 36-37 0.00494867329058211 38-39 0.003919083545561671 40-41 0.0053140115872022655 42-43 0.007207128215143073 44-45 0.006742152201262875 46-47 0.015908822189186784 48-49 0.01796800167922766 50-51 0.017436600520507436 52-53 0.011557975202164929 54-55 0.003919083545561671 56-57 0.003985508690401699 58-59 0.009365945422443994 60-61 0.002490942931501062 62-63 1.9927543452008498E-4 64-65 2.3248800694009914E-4 66-67 7.971017380803399E-4 68-69 9.963771726004249E-5 70-71 1.660628621000708E-4 72-73 0.0033876823868414447 74-75 0.00614432589770262 76-77 0.00597826303560255 78-79 0.003786233255881614 80-81 0.0 82-83 0.001029589745020439 84-85 2.3248800694009914E-4 86-87 3.321257242001416E-4 88-89 2.3248800694009914E-4 90-91 5.314011587202266E-4 92-93 6.310388759802691E-4 94-95 0.0 96-97 0.002424517786661034 98-99 5.646137311402407E-4 100 1.9927543452008498E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1505454.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.740602037203082 #Duplication Level Percentage of deduplicated Percentage of total 1 74.25127811913286 13.91513653984354 2 9.746626408866573 3.6531529346772458 3 4.03690165983367 2.269619024108022 4 2.321706507116887 1.7404071078824954 5 1.54961105609666 1.452032205737874 6 1.0786080276141479 1.2128258279789579 7 0.8879745970195706 1.1648824979322687 8 0.6816350166684543 1.0219400465604633 9 0.540178327149008 0.9110940352399488 >10 4.247380973427064 15.722345397558641 >50 0.4059703620276859 5.129013417878124 >100 0.19657510969804787 7.212238858306429 >500 0.021722974078950665 2.794385509458363 >1k 0.018161830787319408 7.978857910804487 >5k 0.009971201216567518 12.290197460767939 >10k+ 0.005697829266610011 21.531871225265224 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 47200 3.1352668364493366 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 44383 2.948147203434977 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 25367 1.6850066491569986 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23041 1.5305017622590926 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 20311 1.3491611168458153 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 18148 1.205483528556834 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 17664 1.1733337584542602 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 16890 1.1219206963480783 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 16220 1.0774158493052595 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 15916 1.0572226052738907 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15501 1.029656170165279 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 13656 0.9071017779354268 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13483 0.895610227878102 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12828 0.8521017580078832 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 11756 0.780894002739373 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10269 0.6821198123622508 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9517 0.6321681034425496 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 8476 0.56301952766408 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8350 0.5546499594142366 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7670 0.5094808609230173 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 7342 0.48769341341548794 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 7271 0.48297722813184596 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 7165 0.4759361627788029 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6923 0.4598612777275161 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 6856 0.4554107930232342 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6756 0.44876827853923135 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 6630 0.4403987102893878 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6537 0.4342211718192652 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 6533 0.433955471239905 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6511 0.4324941180534244 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6502 0.4318962917498641 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6481 0.43050136370822356 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 6246 0.41489145467081695 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 6206 0.41223444887721583 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6154 0.4087803413455343 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6065 0.40286850345477176 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6009 0.3991486953437302 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 5945 0.39489748607396835 TruSeq Adapter, Index 10 (95% over 21bp) TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 5801 0.3853322652170043 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5661 0.3760327449394003 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 5309 0.35265109395571037 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5176 0.3438165496919866 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5049 0.335380556297303 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 5015 0.333122101372742 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 4984 0.33106292188270114 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4699 0.3121317556032931 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4355 0.28928150577832334 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 4229 0.28091193752847976 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4210 0.27964985977651924 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4152 0.2757972013757976 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3894 0.2586595140070703 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3690 0.24510878445970452 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3666 0.24351458098354384 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 3649 0.24238535352126334 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3354 0.222789935793455 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 3337 0.22166070833117452 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 3278 0.21774162478561285 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3245 0.2155495950058919 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3178 0.21109911030161 No Hit ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 2975 0.19761480589908426 RNA PCR Primer, Index 10 (95% over 23bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2854 0.18957736337344083 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 2715 0.1803442682406769 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2636 0.17509668179831467 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2522 0.16752421528655143 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2514 0.1669928141278312 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2375 0.15775971899506727 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2366 0.15716189269150702 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 2142 0.14228266024734068 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2087 0.1386292772811391 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2034 0.1351087446046176 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 1836 0.121956565926292 TruSeq Adapter, Index 10 (96% over 28bp) TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA 1765 0.11724038064264998 No Hit CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC 1746 0.11597830289068946 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1739 0.11551332687680925 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT 1710 0.11358699767644842 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1625 0.10794086036504603 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1541 0.10236114819848363 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3285028968005664E-4 0.0 10-11 0.0 0.0 0.0 1.9927543452008496E-4 0.0 12-13 0.0 0.0 0.0 2.3248800694009912E-4 0.0 14-15 0.0 0.0 0.0 3.985508690401699E-4 0.0 16-17 0.0 0.0 0.0 6.974640208202974E-4 0.0 18-19 0.0 0.0 0.0 0.001228865179540524 0.0 20-21 0.0 0.0 0.0 0.006775364773682889 0.0 22-23 0.0 0.0 0.0 0.021322471493649093 0.0 24-25 0.0 0.0 0.0 0.04533516135331933 0.0 26-27 0.0 0.0 0.0 0.08974037067887827 0.0 28-29 0.0 0.0 0.0 0.2385326951205417 0.0 30-31 0.0 0.0 0.0 0.5196771206559616 0.0 32-33 0.0 0.0 0.0 0.9937201668068236 0.0 34-35 0.0 0.0 0.0 1.8211449835066364 0.0 36-37 0.0 0.0 0.0 2.886836794747631 0.0 38-39 0.0 0.0 0.0 4.082323339006041 0.0 40-41 0.0 0.0 0.0 5.500466968768225 0.0 42-43 0.0 0.0 0.0 6.6592868330749395 0.0 44-45 0.0 0.0 0.0 7.6909025450129995 0.0 46-47 0.0 0.0 0.0 8.909604677392998 0.0 48-49 0.0 0.0 0.0 10.067959565685833 0.0 50-51 0.0 0.0 0.0 11.144478675535751 0.0 52-53 0.0 0.0 0.0 12.187386662096618 0.0 54-55 0.0 0.0 0.0 13.331061593379804 0.0 56-57 0.0 0.0 0.0 14.581880283289959 0.0 58-59 0.0 0.0 0.0 15.660857123498957 0.0 60-61 0.0 0.0 0.0 16.57961651435381 0.0 62-63 0.0 0.0 0.0 17.409930824854165 0.0 64-65 0.0 0.0 0.0 18.34596075336742 0.0 66-67 0.0 0.0 0.0 19.34788442556199 0.0 68-69 0.0 0.0 0.0 20.54101287717858 0.0 70-71 0.0 0.0 0.0 22.02388781058737 0.0 72-73 0.0 0.0 0.0 23.178190765044963 0.0 74-75 0.0 0.0 0.0 24.23986385502314 0.0 76-77 0.0 0.0 0.0 25.325383571998877 0.0 78-79 0.0 0.0 0.0 26.45793229152136 0.0 80-81 0.0 0.0 0.0 27.58516699945664 0.0 82-83 0.0 0.0 0.0 28.86654125599321 0.0 84-85 0.0 0.0 0.0 30.111115982288396 0.0 86-87 0.0 0.0 0.0 31.21619790441953 0.0 88 0.0 0.0 0.0 32.14591744417299 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 35 3.6428974E-6 67.13109 6 GTACCCA 935 0.0 61.85502 1 ACCGTAT 40 8.035575E-6 58.74946 8 GCGAATT 35 2.9398655E-4 53.706657 2 TGTACCG 45 1.6158681E-5 52.21307 5 ACCCATG 1175 0.0 50.791096 3 TACCCAT 1190 0.0 49.362736 2 CGGAGAT 50 3.0023632E-5 47.01987 1 TACCGTA 50 3.012992E-5 46.991764 7 AGCGAAT 45 0.0010119488 41.795444 1 CGTACAG 60 8.834503E-5 39.1598 5 TTGGCCG 55 0.00271961 34.17583 5 TAAGGCG 125 6.2809704E-9 33.834072 5 TCGTACA 70 2.1875725E-4 33.565544 4 CTTATGT 620 0.0 29.560318 2 GTAGGGC 65 0.006160038 28.918007 6 GGGCGCA 115 4.1978274E-6 28.608433 9 AAGGCGG 150 3.7056452E-8 28.195059 6 GTGTGAT 85 6.8002485E-4 27.658749 1 AGTACGT 155 5.0926246E-8 27.286448 2 >>END_MODULE