##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522952_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1652399 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.597021663653877 33.0 31.0 34.0 30.0 34.0 2 31.86562204407047 34.0 31.0 34.0 28.0 35.0 3 31.868937223999772 34.0 31.0 34.0 28.0 36.0 4 34.058646852243314 35.0 34.0 37.0 32.0 37.0 5 34.184910545213356 35.0 34.0 37.0 32.0 37.0 6 34.56806316150034 35.0 35.0 37.0 32.0 37.0 7 34.49969771223536 35.0 34.0 37.0 32.0 37.0 8 34.700050653625425 36.0 35.0 37.0 32.0 37.0 9 35.731418985366126 37.0 35.0 39.0 32.0 39.0 10-11 35.76530214554717 37.0 34.5 39.0 32.0 39.0 12-13 35.89615008239535 37.5 35.5 39.0 31.5 39.0 14-15 36.41433424977866 38.0 35.5 40.0 31.5 41.0 16-17 36.642425649010924 38.0 36.0 40.0 32.0 41.0 18-19 36.6384577816859 38.0 35.5 40.0 31.5 41.0 20-21 36.8168021161959 38.5 36.0 40.5 31.5 41.0 22-23 36.720227075906 38.0 36.0 40.5 31.5 41.0 24-25 36.657362719294795 38.0 36.0 40.5 31.0 41.0 26-27 36.41643543720373 38.0 36.0 40.0 30.5 41.0 28-29 36.30340129714433 38.0 35.0 40.0 30.5 41.0 30-31 36.17887114431805 38.0 36.0 40.0 30.0 41.0 32-33 35.97146724247594 37.5 35.0 40.0 30.0 41.0 34-35 35.78469758212151 37.5 35.0 40.0 30.0 41.0 36-37 35.614310163586396 37.5 35.0 40.0 29.0 41.0 38-39 35.40028376923492 36.5 35.0 40.0 28.5 41.0 40-41 35.458160226434416 37.0 35.0 40.0 28.5 41.0 42-43 35.15105673629674 36.0 35.0 40.0 27.0 41.0 44-45 34.94649506565908 37.0 34.5 40.0 27.0 41.0 46-47 34.51981240608352 36.5 34.0 40.0 24.0 41.0 48-49 34.183138273504156 37.0 34.0 40.0 22.0 41.0 50-51 33.1214615840363 36.5 32.5 38.5 19.5 40.0 52-53 33.461136807756475 37.0 33.5 38.5 20.5 39.5 54-55 34.34482410120074 37.5 34.0 40.0 20.5 41.0 56-57 34.36609892646994 37.0 34.5 40.0 19.5 41.0 58-59 34.33364278240304 38.0 34.0 40.0 19.5 41.0 60-61 33.8306404809008 37.0 34.0 39.5 18.0 41.0 62-63 33.63508057073382 37.5 33.0 39.0 18.5 41.0 64-65 33.24138298316569 36.0 33.0 39.0 18.0 40.5 66-67 32.83672103408438 36.0 33.0 38.5 18.0 40.0 68-69 32.391918356280776 36.0 32.5 37.5 17.5 39.0 70-71 31.842470250829248 35.5 31.5 37.0 14.0 39.0 72-73 31.305428652522785 35.0 30.5 37.0 12.5 38.0 74-75 30.887322916559498 35.0 30.5 36.0 11.0 37.0 76-77 30.636275802636046 35.0 30.0 36.5 10.0 37.5 78-79 30.2191238314717 34.0 29.0 36.0 10.0 37.0 80-81 29.859727886545564 34.0 29.0 35.5 9.5 37.5 82-83 29.452171055538038 34.0 27.5 35.0 9.0 37.0 84-85 29.127433809872798 34.0 26.5 35.0 9.0 36.5 86-87 28.974260151452526 34.0 27.0 35.0 9.0 37.0 88-89 28.669367386448428 34.0 26.0 35.0 8.0 36.5 90-91 28.3344767214214 34.0 25.0 35.0 8.0 36.0 92-93 27.918673698059607 33.5 25.0 35.0 7.0 35.0 94-95 27.718232400285885 33.5 24.0 35.0 7.0 36.0 96-97 27.432823730830144 33.0 23.0 35.0 7.0 35.0 98-99 27.368512084551007 33.0 21.5 35.0 7.0 36.0 100 25.931023923398648 31.0 18.0 34.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09743328725383549 1101 2 -0.20536134586786403 1101 3 -0.08891656555276839 1101 4 -0.08073668942368073 1101 5 -0.06729578023864491 1101 6 0.062210129178360773 1101 7 -0.036947271710069174 1101 8 -0.05034475137080108 1101 9 -0.08402413888677529 1101 10-11 -0.21157919302610395 1101 12-13 -0.09599435046502691 1101 14-15 -0.3529744926471423 1101 16-17 -0.1925019908581973 1101 18-19 -0.16278147508018748 1101 20-21 -0.2928115546068071 1101 22-23 0.0032944337958085157 1101 24-25 -0.2983120287027461 1101 26-27 -0.26692013237745726 1101 28-29 -0.24574036539151933 1101 30-31 -0.13381495633981189 1101 32-33 -0.2933199113088776 1101 34-35 -0.28828540995373686 1101 36-37 -0.043667402220144425 1101 38-39 -0.015353468807283832 1101 40-41 -0.04277561955017717 1101 42-43 -0.06663899573419485 1101 44-45 -0.11223764556611826 1101 46-47 -0.24333942725883873 1101 48-49 -0.339540435860755 1101 50-51 -0.3907579689152314 1101 52-53 -0.16211068462841638 1101 54-55 -0.06332018477576185 1101 56-57 -0.3077830564754649 1101 58-59 -0.14885438851013788 1101 60-61 -0.15022278317133697 1101 62-63 -0.36324583801862076 1101 64-65 -0.3320622884148108 1101 66-67 -0.25425530219470716 1101 68-69 -0.289743610609726 1101 70-71 -0.5556103270286243 1101 72-73 -0.6064186580803756 1101 74-75 -0.5065327598404892 1101 76-77 -0.5041973726732785 1101 78-79 -0.6530848937556293 1101 80-81 -0.7888734440556711 1101 82-83 -1.182862786591631 1101 84-85 -0.865929814845753 1101 86-87 -0.9222253362284185 1101 88-89 -0.9628939720331786 1101 90-91 -1.0258817919259648 1101 92-93 -0.9536802528549906 1101 94-95 -1.1154730385468454 1101 96-97 -1.2174514811145016 1101 98-99 -1.0522314829380868 1101 100 -1.0035096066181453 1102 1 0.025120252169319457 1102 2 -0.08909548364971442 1102 3 0.014439451358022382 1102 4 -0.060487253589975865 1102 5 -0.06261917155040209 1102 6 -0.18550815014366862 1102 7 -0.09018811684871508 1102 8 0.1117000111114308 1102 9 0.027892088891086075 1102 10-11 0.03506378784319253 1102 12-13 -0.12136471248356173 1102 14-15 -0.045598863962936775 1102 16-17 -0.07095171533123334 1102 18-19 -0.018778341472575732 1102 20-21 -0.0827070063657871 1102 22-23 -0.08212894565634343 1102 24-25 -0.15262098971806637 1102 26-27 -0.18237432174022672 1102 28-29 -0.16592440637563044 1102 30-31 -0.2974054342483825 1102 32-33 -0.24459511087592034 1102 34-35 -0.2085105837136112 1102 36-37 -0.6598526692532971 1102 38-39 -0.2521080886235154 1102 40-41 -0.9375266597709313 1102 42-43 -0.9275958067372372 1102 44-45 -0.311099583091071 1102 46-47 -0.3566763017692125 1102 48-49 -0.39927775402048127 1102 50-51 -0.531465469362054 1102 52-53 -0.14039195263704585 1102 54-55 -0.1436019672572968 1102 56-57 0.09369809928454487 1102 58-59 0.1210407208237072 1102 60-61 0.03630224476554389 1102 62-63 0.017782305987019242 1102 64-65 0.17158036339227323 1102 66-67 0.08544045724578808 1102 68-69 0.22452862739745427 1102 70-71 -2.737982841232167E-4 1102 72-73 -0.029815649082113538 1102 74-75 -7.048510224620941E-4 1102 76-77 -0.20078406219055722 1102 78-79 -0.26234461236568407 1102 80-81 -0.1924492394424533 1102 82-83 -0.17498304128912068 1102 84-85 -0.47052858445377055 1102 86-87 -0.5081393858483274 1102 88-89 -0.2452631698062433 1102 90-91 -0.26456130037050585 1102 92-93 -0.49286412847281724 1102 94-95 -0.6126714162868723 1102 96-97 -0.6499542664506883 1102 98-99 -0.5475987213712159 1102 100 -0.4713692490594852 1103 1 -0.03292526326965728 1103 2 -0.1368623371662352 1103 3 0.0654822117765761 1103 4 0.043262964474664045 1103 5 0.04324750784356013 1103 6 0.1747979961521935 1103 7 0.17072828128300443 1103 8 -0.08087657483566346 1103 9 0.003881937776405664 1103 10-11 -0.034549028103675994 1103 12-13 -0.09486286428288082 1103 14-15 -0.3139084762522373 1103 16-17 -0.19216150503463325 1103 18-19 -0.0275256136073736 1103 20-21 -0.08641245946295584 1103 22-23 -0.20485243311063073 1103 24-25 -0.2341977369506978 1103 26-27 -0.18037100715771004 1103 28-29 -0.4253760684396255 1103 30-31 -0.18774566445880936 1103 32-33 -0.22874345736993718 1103 34-35 -0.387612009280339 1103 36-37 -0.5888409480732122 1103 38-39 -0.5673032441324111 1103 40-41 -0.30692980450989893 1103 42-43 -0.4520770597613364 1103 44-45 -0.4123947224714115 1103 46-47 -0.40427940108489935 1103 48-49 -0.4774247147754309 1103 50-51 -0.5646730109424567 1103 52-53 -0.3512057369554853 1103 54-55 -0.4083764637337026 1103 56-57 -0.20187782177112013 1103 58-59 -0.38484496722372086 1103 60-61 -0.47048121128744214 1103 62-63 -0.442358769491328 1103 64-65 -0.4727430437484017 1103 66-67 -0.4436041069376344 1103 68-69 -0.3779308948681752 1103 70-71 -0.4176128994957402 1103 72-73 -0.3598091647449131 1103 74-75 -0.38074872727365516 1103 76-77 -0.5631317872414954 1103 78-79 -0.6010282096258024 1103 80-81 -0.6292240716561146 1103 82-83 -0.541095055745572 1103 84-85 -0.6140611986949516 1103 86-87 -1.3093870447392746 1103 88-89 -0.8565419666819096 1103 90-91 -1.173086064284675 1103 92-93 -0.6056841897613232 1103 94-95 -1.1493186189701383 1103 96-97 -0.7522450972029056 1103 98-99 -0.8568448266544699 1103 100 -0.7802573991801189 1104 1 0.10838217733355648 1104 2 0.0880034183251368 1104 3 0.09714505706536514 1104 4 0.18817093709309063 1104 5 0.17307096349406947 1104 6 0.14067772365982734 1104 7 0.07987957145297031 1104 8 0.018939138802522848 1104 9 0.1771202164028267 1104 10-11 -0.07681371559436201 1104 12-13 0.16813064764610175 1104 14-15 -0.09224002296733858 1104 16-17 0.00814362619908593 1104 18-19 0.13037173524968892 1104 20-21 0.05115424922232137 1104 22-23 0.014399122736080017 1104 24-25 -0.0018476226402199813 1104 26-27 0.09755317405831221 1104 28-29 0.1154179675640421 1104 30-31 0.05430553869622656 1104 32-33 0.1460075740712341 1104 34-35 -0.0584189155523589 1104 36-37 0.09624525625635982 1104 38-39 0.2037143342058414 1104 40-41 0.36254458233265296 1104 42-43 0.1035107541789202 1104 44-45 0.111680785308657 1104 46-47 0.1427690394289769 1104 48-49 0.30943503900211056 1104 50-51 0.11161823429736017 1104 52-53 0.17607327656656935 1104 54-55 0.41772685573680235 1104 56-57 0.3376167803258383 1104 58-59 0.3617327490149833 1104 60-61 0.29650530967058586 1104 62-63 0.4186137959931884 1104 64-65 0.217280761285231 1104 66-67 0.3423247029430314 1104 68-69 0.07092550403735487 1104 70-71 0.15961739559403654 1104 72-73 0.2421049981748169 1104 74-75 0.07412592822333863 1104 76-77 0.10142278236548208 1104 78-79 0.20785244096791544 1104 80-81 0.16962422129634191 1104 82-83 0.16308066808295152 1104 84-85 0.05720435563643633 1104 86-87 0.10710509579478966 1104 88-89 0.17889056035723172 1104 90-91 -0.09165804349373374 1104 92-93 0.04942694450965135 1104 94-95 -0.11375416340895939 1104 96-97 -0.07876281284607245 1104 98-99 -0.27430637300563276 1104 100 0.15286269795897311 1105 1 -0.07019932294845077 1105 2 -0.06712387872755343 1105 3 -0.0011510905756075829 1105 4 -0.028004511168298052 1105 5 0.030578276024399997 1105 6 0.056027806950531556 1105 7 0.016308105405407503 1105 8 -0.09389353214017149 1105 9 -0.13138002625512968 1105 10-11 0.020606895903689804 1105 12-13 -0.03846309046694074 1105 14-15 -0.06431632249907437 1105 16-17 -0.0059764435814742 1105 18-19 -0.001145598398444747 1105 20-21 -0.05624321139494981 1105 22-23 -0.07661728618848684 1105 24-25 -0.20956447359576913 1105 26-27 -0.04123815977344947 1105 28-29 -0.3061813779153013 1105 30-31 -0.15836448583190332 1105 32-33 -0.13617657708499564 1105 34-35 -0.34325344905882815 1105 36-37 -0.41403983405174927 1105 38-39 -0.16091695686565544 1105 40-41 -0.27415464640854736 1105 42-43 -0.4968422475656027 1105 44-45 -0.5394688887426824 1105 46-47 -0.5491594682901635 1105 48-49 -0.4641372140442286 1105 50-51 -0.7962078127389276 1105 52-53 -0.5959569507449203 1105 54-55 -0.5186606886958387 1105 56-57 -0.6467621136785411 1105 58-59 -0.6045164803820384 1105 60-61 -0.3748562965939257 1105 62-63 -0.45269222848246926 1105 64-65 -0.38274104513930496 1105 66-67 -0.26950372476456863 1105 68-69 -0.1392441597829972 1105 70-71 -0.4157426563478843 1105 72-73 -0.33237459888180965 1105 74-75 -0.3484809396526849 1105 76-77 -0.24411559038337316 1105 78-79 -0.04433230043740366 1105 80-81 -0.35486947615509834 1105 82-83 -0.3307930382878048 1105 84-85 -0.4431342177971054 1105 86-87 -0.48339074622832 1105 88-89 -0.37421300212391273 1105 90-91 -0.4945014045031897 1105 92-93 -0.5080395424589597 1105 94-95 -0.4926268532396243 1105 96-97 -0.5682670364061906 1105 98-99 -0.30593716564457196 1105 100 -0.28436267225836787 1106 1 -0.08975684839167286 1106 2 -0.08679705519226033 1106 3 -0.08269131212545489 1106 4 -0.16907375174743322 1106 5 -0.16866030265580179 1106 6 -0.08634172619442637 1106 7 -0.04015772440629917 1106 8 -1.3042189803660236 1106 9 -0.05095255507411878 1106 10-11 -0.15130678467443914 1106 12-13 -0.15041640496270503 1106 14-15 -0.2770001138521323 1106 16-17 -0.40207025218840897 1106 18-19 -0.12931818723092903 1106 20-21 -0.218542228673833 1106 22-23 -0.21474367712644948 1106 24-25 -0.2167379626307948 1106 26-27 -0.26152209865970377 1106 28-29 -0.37938066025383677 1106 30-31 -0.22509199952248338 1106 32-33 -0.2421629240130585 1106 34-35 -0.2358542842467486 1106 36-37 -0.1526091371449496 1106 38-39 -0.2166779405869974 1106 40-41 -0.19065929745503496 1106 42-43 -0.13729416174628994 1106 44-45 -0.10062796203417435 1106 46-47 -0.11388184332454898 1106 48-49 -0.24148399206928417 1106 50-51 -0.19168429494742867 1106 52-53 -0.1990739020589558 1106 54-55 -0.18191148262447143 1106 56-57 -0.29422185462025396 1106 58-59 -0.25951698049457406 1106 60-61 -0.1519686468727386 1106 62-63 -0.09360362104579423 1106 64-65 -0.1822224909646124 1106 66-67 -0.20294187334800284 1106 68-69 -0.13300838173294238 1106 70-71 -0.028813409767401765 1106 72-73 0.02995536285958522 1106 74-75 -0.1990566941974592 1106 76-77 -0.13378139018833224 1106 78-79 -0.11708773290970953 1106 80-81 -0.23857768550220015 1106 82-83 -0.23870981956359216 1106 84-85 -0.2786628367363697 1106 86-87 -0.35105003206763286 1106 88-89 -0.41753212392631767 1106 90-91 -0.5907996341048509 1106 92-93 -0.43480744023596074 1106 94-95 -0.5167682225477144 1106 96-97 -0.6308576818014053 1106 98-99 -0.6393356177963057 1106 100 -0.6223199626487066 1107 1 -0.04650726093041513 1107 2 -0.04067491936806533 1107 3 -0.08164778700474074 1107 4 0.14692788014014013 1107 5 0.03838543282009255 1107 6 0.06433614650616448 1107 7 0.08885881976731014 1107 8 0.08534165826221596 1107 9 0.11161282698851949 1107 10-11 0.04530446073587058 1107 12-13 0.14033131009023947 1107 14-15 0.0031057000517975553 1107 16-17 0.0700205885381493 1107 18-19 0.03971765949342654 1107 20-21 0.14794288035208325 1107 22-23 0.056516149211482514 1107 24-25 0.20293952645442914 1107 26-27 0.2133826825273175 1107 28-29 0.1351327875957935 1107 30-31 0.021598556841574634 1107 32-33 0.10719079066236503 1107 34-35 0.19034618826994887 1107 36-37 0.25321481384971634 1107 38-39 0.09330191960651746 1107 40-41 0.015761540774981597 1107 42-43 -0.006279620897338134 1107 44-45 0.09528417482517426 1107 46-47 0.1384746510399637 1107 48-49 0.09443214839482295 1107 50-51 0.03693676005570978 1107 52-53 0.05490572674750638 1107 54-55 0.17429859549909565 1107 56-57 0.20043949826325758 1107 58-59 0.13783165888872162 1107 60-61 -0.042904287784402584 1107 62-63 -0.21315118326150895 1107 64-65 -0.11250039898217068 1107 66-67 -0.10458753758104677 1107 68-69 -0.03603894309008382 1107 70-71 -0.188138231508038 1107 72-73 -0.14198005275417103 1107 74-75 0.062030094211269926 1107 76-77 -0.044542105539640886 1107 78-79 0.03766833643333456 1107 80-81 0.2054677516061929 1107 82-83 0.100279430072586 1107 84-85 0.12328347842496257 1107 86-87 -0.0704620349660594 1107 88-89 -0.05796236682182254 1107 90-91 -0.15652382988264435 1107 92-93 -0.10379799903566678 1107 94-95 -0.002356387100437729 1107 96-97 0.057580425209010855 1107 98-99 0.20522062143353992 1107 100 0.14441525750388706 1108 1 -0.15035233414318228 1108 2 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0.15154611990713107 2316 42-43 0.13713451824665412 2316 44-45 0.00861951723681642 2316 46-47 -1.4745564351903582 2316 48-49 0.061835363601169036 2316 50-51 0.1911353932014137 2316 52-53 0.11342779425935134 2316 54-55 -0.003669987964485699 2316 56-57 0.017058662146155257 2316 58-59 -0.08158574245361194 2316 60-61 0.037422408780066974 2316 62-63 0.0067856840065232404 2316 64-65 -0.2075003479039097 2316 66-67 -0.08794896395854579 2316 68-69 -0.009293173221486484 2316 70-71 0.04170305130548613 2316 72-73 0.04121128301048671 2316 74-75 0.10239060458526694 2316 76-77 0.050807218694949086 2316 78-79 -0.15294688420772928 2316 80-81 0.13562674121006424 2316 82-83 0.24347018695027245 2316 84-85 0.29661858247138184 2316 86-87 0.29405066138729197 2316 88-89 0.4803357735889122 2316 90-91 0.7439821854340245 2316 92-93 0.8916851502456602 2316 94-95 0.9449117771808737 2316 96-97 0.9375504252430638 2316 98-99 1.013513688070244 2316 100 0.6557958348244597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 2870.0 1 263.0 2 170.0 3 138.0 4 111.0 5 98.0 6 134.0 7 366.0 8 1045.0 9 2274.0 10 3787.0 11 4723.0 12 4956.0 13 4311.0 14 4351.0 15 5350.0 16 7485.0 17 10817.0 18 13574.0 19 17228.0 20 21641.0 21 24759.0 22 23362.0 23 20729.0 24 19482.0 25 19966.0 26 22305.0 27 25758.0 28 30618.0 29 36984.0 30 45367.0 31 57587.0 32 73782.0 33 98678.0 34 139777.0 35 226542.0 36 245630.0 37 257740.0 38 168356.0 39 9256.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.54528265296228 16.914876854455542 13.03324687287889 16.506593619703285 2 14.099230941129662 26.666884684773066 30.94367320539304 28.290211168704225 3 31.225750372697654 26.156455317557825 29.68900991108672 12.928784398657802 4 13.629573515870314 17.901213210500856 37.100191994036834 31.369021279591998 5 9.983871721610255 42.043514180077054 33.52272071976919 14.449893378543502 6 26.63867213451937 32.838787534606375 15.23711926920559 25.285421061668668 7 20.280399120992197 37.04786127111518 22.534585753618142 20.137153854274484 8 25.924130504027058 22.930061126363206 20.912254091522698 30.233554278087034 9 22.559368755866792 22.405732360788257 25.347578072712736 29.687320810632222 10-11 25.947239063948818 24.253633217678217 23.0783066732062 26.720821045166765 12-13 34.223664041319566 23.573974814747853 22.878955046530315 19.32340609740227 14-15 27.944784131806717 24.828307170110936 30.09062227334803 17.13628642473432 16-17 19.0529730319937 28.871492892899873 31.573661949830985 20.501872125275437 18-19 19.745096956706647 32.28236991396658 29.495247565237914 18.47728556408886 20-21 23.06990641731507 24.93770760340356 26.27698654654108 25.715399432740288 22-23 19.389829398037072 26.740855236139005 27.527681947421133 26.341633418402786 24-25 26.592497913188645 27.34641917731102 23.651610484528476 22.409472424971852 26-27 24.309985820120264 21.175974582376003 32.629162022187344 21.884877575316395 28-29 19.618279902590665 30.562906554745428 29.11314188783366 20.705671654830244 30-31 23.029344226346264 25.604413902543406 27.312674986662266 24.053566884448067 32-33 20.56982298705254 26.934384240278487 31.133815915755957 21.361976856913017 34-35 22.191833252508378 25.426711783065215 29.471477586315135 22.909977378111268 36-37 22.265491235067618 24.206621584527493 32.674369757457285 20.853517422947608 38-39 23.243701006649932 25.539783722530057 30.18041928176856 21.036095989051454 40-41 20.11567497546495 28.810592466413148 28.540380820342776 22.53335173777912 42-43 20.816747599688057 28.278924847099834 24.831630094890166 26.072697458321947 44-45 24.28800839483633 22.88887911647987 26.98227167061186 25.840840818071946 46-47 24.764852442310637 28.113314842812887 29.461586171233943 17.660246543642533 48-49 23.060758492333303 25.915296270143433 30.4306587852184 20.593286452304863 50-51 15.992299825178009 28.009158440165628 34.362439597848756 21.63610213680761 52-53 19.490341329644593 30.871858808683093 24.719522405445744 24.91827745622657 54-55 17.802584320486563 26.11093228908009 27.0121043132784 29.074379077154948 56-57 23.500313829775937 23.077819166702312 25.86130271058524 27.56056429293651 58-59 27.44007151890613 22.298815915565466 27.025962006036153 23.235150559492258 60-61 22.697689879886855 25.440498771935466 31.2864681179403 20.57534323023738 62-63 18.046843879723912 26.130739707109708 31.480260653003793 24.342155760162587 64-65 16.837894902893478 31.27867539543378 28.266765360789712 23.616664340883027 66-67 21.858774631008902 25.43458277080837 24.426588595785994 28.28005400239674 68-69 20.89681478263191 24.651908873122863 26.90216797695129 27.549108367293933 70-71 24.97864451947817 25.494969210176464 24.581819483905935 24.944566786439427 72-73 23.87147485262096 21.36488547301655 31.60292254349294 23.160717130869553 74-75 21.385617202531417 28.932418323142784 26.28336126365832 23.398603210667474 76-77 21.371837124303738 24.39536939887991 27.78051188568287 26.45228159113348 78-79 22.841139506850446 24.71727898487735 29.580477906452135 22.86110360182007 80-81 24.61154875707737 23.75101018558292 27.435396660138377 24.20204439720133 82-83 21.52920349686523 23.158646822982952 30.22018479443099 25.09196488572083 84-85 21.815088527582642 26.107140949893438 27.602970662342642 24.474799860181278 86-87 22.724463464622115 25.609246597682283 26.374557596297233 25.291732341398365 88-89 22.489710510632978 24.7026031103327 25.05073437399487 27.756952005039455 90-91 25.392243783114292 21.85256791863054 25.96485447156396 26.79033382669121 92-93 25.792300626487197 24.7053216829123 27.401200554774253 22.10117713582626 94-95 23.570712075543373 23.73370730401887 28.04079249906396 24.654788121373798 96-97 21.48210696739616 24.184630900829173 29.662073761578345 24.671188370196322 98-99 25.53496028488338 25.12151776000796 24.08736536875809 25.25615658635057 100 24.884391309624952 19.840393251608205 25.46813933729822 29.807076101468628 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3288.0 1 2631.0 2 1759.5 3 1200.5 4 757.5 5 722.0 6 945.5 7 1453.0 8 2106.0 9 2467.5 10 2114.5 11 1641.5 12 1611.5 13 1652.5 14 1679.0 15 1720.5 16 1832.5 17 1993.0 18 2191.0 19 2459.5 20 2749.0 21 3094.5 22 3540.5 23 4049.5 24 4709.5 25 5515.5 26 6387.5 27 7368.5 28 8424.0 29 9441.5 30 10556.5 31 11646.5 32 12507.0 33 13203.0 34 13206.5 35 12887.0 36 12671.0 37 12203.5 38 11704.0 39 11152.5 40 10455.5 41 10311.0 42 10583.5 43 11336.0 44 12484.0 45 16162.0 46 39804.0 47 121412.5 48 261432.0 49 289442.0 50 245437.5 51 185509.0 52 84615.0 53 41470.5 54 26894.0 55 17678.5 56 11863.5 57 8330.0 58 6251.0 59 5063.0 60 4307.0 61 3856.5 62 3394.0 63 2553.0 64 1874.0 65 1604.5 66 1229.0 67 917.0 68 759.5 69 618.0 70 529.5 71 434.0 72 350.5 73 298.0 74 296.5 75 272.5 76 204.5 77 167.5 78 142.0 79 115.5 80 83.5 81 59.0 82 52.5 83 40.5 84 27.5 85 20.5 86 13.0 87 15.0 88 12.5 89 5.5 90 5.0 91 2.5 92 0.5 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14034140664573144 2 0.07020096235836502 3 0.29962496951402173 4 0.29968548758502034 5 0.30150102971497805 6 0.2553862596140521 7 0.2527839825611127 8 0.2526024283481169 9 0.263374644985866 10-11 0.23986337440291358 12-13 0.252511651241619 14-15 0.22648888071222506 16-17 0.23429571187104326 18-19 0.2472768381002409 20-21 0.24957652479818737 22-23 0.23856223587644387 24-25 0.2391068985154312 26-27 0.23822938648595163 28-29 0.24794253688122542 30-31 0.2348403745100306 32-33 0.2447955971892987 34-35 0.22842545898417999 36-37 0.23762420577596574 38-39 0.2397120792254171 40-41 0.2569899884955147 42-43 0.24325238637883467 44-45 0.22794131441619125 46-47 0.23036203725613486 48-49 0.24031725993540304 50-51 0.23390234440955243 52-53 0.25048429586316623 54-55 0.2582608679864851 56-57 0.2549323740815626 58-59 0.25196698860263167 60-61 0.23420493476454537 62-63 0.2606815908264287 64-65 0.25472056083306754 66-67 0.2616801389979055 68-69 0.26694521117478287 70-71 0.28416260237388186 72-73 0.2942086021596479 74-75 0.27526644593708904 76-77 0.2718471749256687 78-79 0.2687304942692413 80-81 0.25338916327109856 82-83 0.2633443859503667 84-85 0.2754177411145855 86-87 0.26879101234023983 88-89 0.27844364466451504 90-91 0.27281546406164614 92-93 0.2518156934251352 94-95 0.2722102833516602 96-97 0.27202872913866444 98-99 0.25938045229995904 100 0.2783226085225179 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1652399.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.796896835981345 #Duplication Level Percentage of deduplicated Percentage of total 1 76.45837414308838 12.078050485852309 2 8.931866108434093 2.8219153493546307 3 3.9622803431956366 1.8777520145009468 4 2.294106912178918 1.4495908088960834 5 1.5178475009011065 1.198864019224344 6 1.063304110284655 1.0078143201264975 7 0.7984714522876935 0.8829359810785427 8 0.6318632064885222 0.7985182309881251 9 0.47249816024543145 0.6717604223329255 >10 3.3497986130766906 10.031370765497625 >50 0.27087420641456134 2.961060618172571 >100 0.18949732830804794 6.1861621458128795 >500 0.019866655387134058 2.294470419405801 >1k 0.022158961777957217 8.831580681606832 >5k 0.006112817042195094 6.545197458692176 >10k+ 0.011079480888978609 40.36295627845773 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 90636 5.485115883028251 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 58110 3.5167051057280965 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 38605 2.3363001309005873 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 37074 2.243646964201745 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 31568 1.910434465283506 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 26235 1.5876915926480228 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 25433 1.539156099707153 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 25273 1.5294732083473785 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 24864 1.504721317308955 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 23864 1.4442032463103645 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 22854 1.383079994601788 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 21192 1.2824989606021306 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 20540 1.2430411783110495 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 19500 1.1801023844725154 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 18494 1.1192212050479333 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 16741 1.0131330265874041 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 15732 0.9520702929498261 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 15648 0.9469867749859447 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 14675 0.8881026919043161 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 14000 0.8472529939802674 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13813 0.835936114703531 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 13271 0.803135320222295 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 13225 0.8003514889563598 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 12008 0.7267009965510751 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 11953 0.7233725026461526 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 11630 0.7038251657136079 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 11227 0.6794363831011759 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10333 0.625333227628436 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 10292 0.6228519867174938 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9881 0.5979790595370731 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 9678 0.5856938911243592 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8655 0.523783904492801 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 8339 0.5046601940572465 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8068 0.4882597968166284 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7482 0.45279620721145436 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 6618 0.40050859386867216 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 6292 0.38077970272313166 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 5977 0.3617165103585756 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5725 0.3464659564669308 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 5496 0.33260731820825357 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 5418 0.3278869086703635 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5372 0.32510307740442834 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 5349 0.32371116177146075 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5065 0.30652402960786107 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 5035 0.30470848747790336 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 4866 0.29448093347914156 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 4826 0.2920602106391979 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 4806 0.29084984921922613 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4738 0.2867346203913219 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 4691 0.2838902710543882 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4555 0.27565981339857987 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 4476 0.2708788857896912 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4243 0.2567781752470196 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 4099 0.2480635730232226 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 4017 0.2431010912013382 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3973 0.2404382960774002 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3951 0.2391068985154312 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 3846 0.23275250106057918 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 3815 0.2308764408596229 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3567 0.21586795925197244 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3531 0.2136893086960232 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3278 0.1983782367333798 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3153 0.19081347785855596 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 3053 0.1847616707586969 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3022 0.1828856105577406 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 2880 0.17429204447594074 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 2804 0.16969267108004787 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2804 0.16969267108004787 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 2789 0.168784900015069 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2691 0.16285412905720711 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2431 0.1471194305975736 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2286 0.13834431030277797 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 2279 0.13792068380578784 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 2171 0.13138473213794005 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 2041 0.12351738290812327 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2030 0.12285168412713879 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1939 0.11734453966626705 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1919 0.11613417824629522 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1829 0.11068755185642208 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1828 0.11062703378542349 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1741 0.10536196160854613 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.051807099859054E-5 7 0.0 0.0 0.0 0.0 6.051807099859054E-5 8 0.0 0.0 0.0 6.051807099859054E-5 6.051807099859054E-5 9 0.0 0.0 0.0 2.4207228399436215E-4 6.051807099859054E-5 10-11 0.0 0.0 0.0 2.4207228399436215E-4 3.631084259915432E-4 12-13 0.0 0.0 0.0 3.3284939049224794E-4 4.841445679887243E-4 14-15 0.0 0.0 0.0 6.051807099859053E-4 4.841445679887243E-4 16-17 0.0 0.0 0.0 0.0010590662424753343 4.841445679887243E-4 18-19 0.0 0.0 0.0 0.0017247650234598303 4.841445679887243E-4 20-21 0.0 0.0 0.0 0.006656987809844959 4.841445679887243E-4 22-23 0.0 0.0 0.0 0.021453656169000343 5.144036034880195E-4 24-25 0.0 0.0 0.0 0.04148513766953381 5.446626389873148E-4 26-27 0.0 0.0 0.0 0.07446748636376566 5.446626389873148E-4 28-29 0.0 0.0 0.0 0.18560892375267718 7.262168519830864E-4 30-31 0.0 0.0 0.0 0.3865591785034971 7.86734922981677E-4 32-33 0.0 0.0 0.0 0.7568389959083732 8.775120294795628E-4 34-35 0.0 0.0 0.0 1.3442576520561924 9.07771064978858E-4 36-37 0.0 0.0 0.0 2.082093973671008 9.682891359774486E-4 38-39 0.0 0.0 0.0 2.9328570157691933 0.0012708794909704012 40-41 0.0 0.0 0.0 3.904504904687064 0.0012708794909704012 42-43 0.0 0.0 0.0 4.743618218118021 0.001361656597468287 44-45 0.0 0.0 0.0 5.571535688414238 0.001936578271954897 46-47 0.0 0.0 0.0 6.586302702918605 0.002057614413952078 48-49 0.0 0.0 0.0 7.446869672518562 0.002057614413952078 50-51 0.0 0.0 0.0 8.293426708682347 0.002057614413952078 52-53 0.0 0.0 0.0 9.119558895884104 0.002057614413952078 54-55 0.0 0.0 0.0 10.054169725350839 0.002057614413952078 56-57 0.0 0.0 0.0 11.243622151792636 0.002057614413952078 58-59 0.0 0.0 0.0 12.142164210944209 0.002057614413952078 60-61 0.0 0.0 0.0 12.996951704763802 0.002057614413952078 62-63 0.0 0.0 0.0 13.801509199654564 0.0021181324849506686 64-65 0.0 0.0 0.0 14.682622054358543 0.0021181324849506686 66-67 0.0 0.0 0.0 15.694998605058464 0.0021181324849506686 68-69 0.0 0.0 0.0 16.905329765994775 0.0021181324849506686 70-71 0.0 0.0 0.0 18.24471571333558 0.0021181324849506686 72-73 0.0 0.0 0.0 19.285626534511337 0.0021181324849506686 74-75 0.0 0.0 0.0 20.404726703417275 0.0021181324849506686 76-77 0.0 0.0 0.0 21.410567302449348 0.0021181324849506686 78-79 0.0 0.0 0.0 22.818399188089558 0.0021181324849506686 80-81 0.0 0.0 0.0 24.133789720279424 0.0021181324849506686 82-83 0.0 0.0 0.0 25.35922013992988 0.0021181324849506686 84-85 0.0 0.0 0.0 26.64610666067941 0.0021181324849506686 86-87 0.0 0.0 0.0 27.801396636042504 0.0021181324849506686 88 0.0 0.0 0.0 28.83425855377545 0.0021181324849506686 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCACTT 20 0.0021612043 70.51597 5 GTACCCA 1045 0.0 59.85718 1 TACCCAT 1215 0.0 51.4649 2 GTGATCG 50 3.0146148E-5 46.98781 8 ACCCATG 1345 0.0 46.142452 3 CTTAACG 25 0.0017019913 37.602234 20-21 TGATCGC 65 1.4156612E-4 36.145565 9 CCGGATC 155 4.1836756E-11 33.362392 94 TTATGTA 825 0.0 31.344238 3 ACCTAGC 60 0.004166787 31.325205 8 TATAAGG 80 4.772888E-4 29.38433 2 TATGTAC 865 0.0 29.34947 4 CTTATGT 915 0.0 28.260342 2 GTTCTTA 100 5.30131E-5 28.218388 1 GTACCTA 85 6.7914225E-4 27.665085 1 GGCTTAC 170 4.0508894E-9 27.665085 1 CGGAGAT 70 0.008824743 26.864857 4 CGTCATG 70 0.008827358 26.863226 94 AACCGGT 70 0.0088456785 26.851809 7 GCCGTAT 985 0.0 25.29507 94 >>END_MODULE