FastQCFastQC Report
Mon 6 Jun 2016
ERR522951_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522951_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7410858
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184920.24952576341362903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153190.2067102081837218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91850.1239397651392052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG276450.027.511521
TACATGG279100.026.6099132
GTATCAA287600.026.4122141
TACCTGG73600.026.3932842
ACATGGG280900.025.7363073
GGTATCA211850.025.2659111
CATGGGG167500.024.3179654
TCAACGC330000.022.6909794
CAACGCA330300.022.6117295
ATCAACG335250.022.279523
AACGCAG340200.021.9837516
TATCAAC341700.021.9138772
ACCTGGG87000.021.5713713
ATGGGGG98550.020.3303495
GAGTACT186050.019.64138612-13
ACGCAGA380500.019.6164637
TATAACG6600.019.2415922
CGCAGAG390500.019.0407438
GTACCTG109400.018.3152751
GCCGTAT34550.018.08885894
Produced by FastQC (version 0.11.5)