FastQCFastQC Report
Mon 6 Jun 2016
ERR522951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7410858
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182550.2463277531427535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163720.2209190892606497No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT121190.16353032266979073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94020.12686790112561866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG70250.025.6941872
GGTATCA221700.025.6288851
GTACATG275400.025.4649121
TACATGG277850.024.6659072
GTATCAA296800.024.4846041
ACATGGG280000.023.669423
TATAACG5100.023.041982
CATGGGG158500.022.033634
TAACGCA6450.021.8619084
TCAACGC336150.021.2398384
ACCTGGG82650.021.2125343
CAACGCA336400.021.1821375
ATCAACG341750.020.905553
AACGCAG346200.020.648186
TATCAAC349400.020.5565622
GAGTACT190050.019.07839612-13
ATGGGGG92300.018.4855125
ACGCAGA390050.018.3266357
GTATAGG20400.017.9842741
CGCAGAG399550.017.8552368
Produced by FastQC (version 0.11.5)