FastQCFastQC Report
Mon 6 Jun 2016
ERR522950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11192097
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407550.3641408754766868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325870.2911608074876406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210220.18782896538512844No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136520.12197892852429709No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT128640.11493824615708745No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA115070.102813619288682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG195000.053.8275342
ACCTGGG218700.047.177263
GTACCTG234400.045.315791
CCTGGGG195200.035.4599274
TGGGGGG248900.028.3189056
ATGGGGG233400.028.2435975
CATGGGG347300.028.1081584
GTACATG574050.027.2228181
CTGGGGG135350.026.6793865
TACATGG584550.026.4035192
ACATGGG586850.025.6429393
GTATCAA594950.023.214471
GGTATCA447050.022.308121
GGGTTAG44900.022.0016941
TATCACG10250.020.6458852
GTGGTTA75750.020.431321
GAGTACT338950.020.37193912-13
TCAACGC674200.020.2891964
CAACGCA678600.020.121285
ATCAACG681400.020.0005133
Produced by FastQC (version 0.11.5)